Anti-inflammatory and antioxidant potential capacities of AD-MSCs and BM-MSCs in suppressing pancreatic β-cells auto-immunity and apoptosis in rats with T1DM induced model

El-Sawah, Shady G.; Althobaiti, Fayez; Rashwan, Hanan M.; Aldhahrani, Adil; Abdel-Dayem, Marwa A.; Fayad, Eman; Amen, Rehab M.; Shabana, El Shaimaa; El-Hallous, Ehab I.

doi:10.32604/biocell.2022.017853

Cited by 3 publications

(3 citation statements)

References 50 publications

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“…ADMSC isolation and primary culture were conducted from both young and old C57BL/6 mice as described previously [ 9 , 43 ]. Briefly, both young and old mice were sacrificed, and the adipose tissues on the inguinal were collected for ASMSC isolation.…”

Section: Methodsmentioning

confidence: 99%

“…Adipose tissue-derived mesenchymal stem cells (ADMSCs) demonstrate tissue repair potentials through differentiate into target cells in the injured area, secretion of various cytokines/growth factors to neighboring cells as well as immunomodulatory effects to reduce the inflammatory response in damaged tissues. Comparing with other MSCs, ADMSCs are relatively easy to obtain and demonstrate a higher yield and stable quality, thus are more commonly used to treat various disorders, including tendinopathy [ 8 , 9 ]. Even MSCs are regarded as immunogenic and provide differentiation potential, considerable heterogeneity and a limited amount of cells infiltrating to target organs in MSC allogeneic transplantation [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a “One-Stone-Two-Birds” manner

et al. 2023

J Nanobiotechnol

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Background Tendinopathy is the leading sports-related injury and will cause severe weakness and tenderness. Effective therapy for tendinopathy remains limited, and extracellular vesicles (EVs) derived from adipose tissue-derived mesenchymal stem cells (ADMSCs) have demonstrated great potential in tendinopathy treatment; however, the influence of aging status on EV treatment has not been previously described. Results In this study, it was found that ADMSCs derived from old mice (ADMSCold) demonstrated remarkable cellular senescence and impaired NAD+ metabolism compared with ADMSCs derived from young mice (ADMSCyoung). Lower NAMPT contents were detected in both ADMSCold and its secreted EVs (ADMSCold-EVs). Advanced animal experiments demonstrated that ADMSCyoung-EVs, but not ADMSCold-EVs, alleviated the pathological structural, functional and biomechanical properties in tendinopathy mice. Mechanistic analyses demonstrated that ADMSCyoung-EVs improved cell viability and relieved cellular senescence of tenocytes through the NAMPT/SIRT1/PPARγ/PGC-1α pathway. ADMSCyoung-EVs, but not ADMSCold-EVs, promoted phagocytosis and M2 polarization in macrophages through the NAMPT/SIRT1/Nf-κb p65/NLRP3 pathway. The macrophage/tenocyte crosstalk in tendinopathy was influenced by ADMSCyoung-EV treatment and thus it demonstrated "One-Stone-Two-Birds" effects in tendinopathy treatment. Conclusions This study demonstrates an effective novel therapy for tendinopathy and uncovers the influence of donor age on curative effects by clarifying the detailed biological mechanism. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a “One-Stone-Two-Birds” manner

et al. 2023

J Nanobiotechnol

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“…For example, MSCs injection significantly decreased the levels of increased ROS, XO, and MDA levels. Further, the mRNA expression of various antioxidants was increased such as GSH, GSH-Px-1, GSH-Px-3 and GSH-Px-4, HO-1, CAT, SOD-1, and SOD-3, and TAC in the pancreas, liver, kidney, and heart tissues of STZ-diabetic rats compared to diabetic untreated rats (Abdel Fattah et al, 2020;Aminzadeh et al, 2020;El-Sawah et al, 2021a, 2021b.…”

Section: Oxidative Stress and Antioxidantsmentioning

confidence: 99%

The potency of , -diphenyl-1,4-phenylenediamine and adipose-derived stem cell co-administration in alleviating hepatorenal dysfunction complications associated with type 1 diabetes mellitus in rats

M. ABD EL-LATEEF,

H. QAHL,

FAYAD

et al. 2023

Biocell

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Background: The increasing occurrence of diabetes mellitus (DM) noted worldwide has considerably elicited concern in the recent past. DM is associated with elevated vascular complications, morbidity, mortality, and poor quality of life. In this context, mesenchymal stem cells (MSCs) have shown significant therapeutic potentialities in managing and curing type 1 DM owing to their self-renewable, immunosuppressive, and differentiation capacities. We investigated the potential action of N, N′-diphenyl-1,4-phenylenediamine (DPPD), a well-known synthetic antioxidant to enhance the therapeutic ability of the adipose-derived stem cells (AD-MSCs) in alleviating kidney and liver complications in diabetic rats. Methods: Over the four weeks of experiments, albino male rats (n = 36) were split into six test groups: control, DPPD (250 mg/kg, i.p.), STZ-diabetic (D), D+DPPD, D+AD-MSCs (1 × 10 6 cell/rat, i.v.), and D+AD-MSCs +DPPD treated groups. Results: Significant declines in the renal and hepatic oxidative stress markers (MDA, ROS, and AGEs) were observed coupled with a significant elevation in many antioxidant marker levels (GSH, SOD, CAT, GPx, HO-1, and TAC) in the diabetic rats treated with either DPPD or AD-MSCs or their co-administered injection compared to the diabetic untreated rats. This was suggested to be the leading cause of amelioration of the kidney functions (as measured by urea, uric acid, and creatine levels) and liver functions (as evidenced by the levels of AST, ALT, ALP, bilirubin, total proteins, albumin, and globulins). Conclusion: DPPD and AD-MSCs co-administration showed superior results in terms of the enhancement of the relative hepato-renal function, indicating the beneficial role of DPPD supplementation in increasing the therapeutic potential of AD-MSCs.

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Rutin and quercetagetin enhance the regeneration potential of young and aging bone marrow-derived mesenchymal stem cells in the rat infarcted myocardium

et al. 2022

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Anti-inflammatory and antioxidant potential capacities of AD-MSCs and BM-MSCs in suppressing pancreatic β-cells auto-immunity and apoptosis in rats with T1DM induced model

Cited by 3 publications

References 50 publications

NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a “One-Stone-Two-Birds” manner

NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a “One-Stone-Two-Birds” manner

The potency of , -diphenyl-1,4-phenylenediamine and adipose-derived stem cell co-administration in alleviating hepatorenal dysfunction complications associated with type 1 diabetes mellitus in rats

Rutin and quercetagetin enhance the regeneration potential of young and aging bone marrow-derived mesenchymal stem cells in the rat infarcted myocardium

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