Anti-inflammatory and Anti-arthritis Activity of Flavonoids Fractions Isolated from Centipeda minima Leaves Extracts in Rats

Abstract

Centipeda minima has been used since centuries as a traditional medicinal plant in treating a number of disease conditions, the bioactive components responsible for its anti-inflammatory and anti-arthritis activity have not been identified. The present study was planned to isolate flavonoids fractions from Centipeda minima leaves extracts, and assessed anti-inflammatory and anti-arthritis activity in rats. The hydroalcoholic and aqueous extracts of Centipeda minima leaves were prepared and evaluated for in vitro antioxidant activity namely DPPH, total polyphenol content, total flavonol content and reducing power assay. The different fractions were isolated from hydroalcoholic extracts by using column chromatography. The Flavonoids fractions were investigated for anti-inflammatory and anti-arthritis activity in carrageenan rat induced paw oedema, cotton pellet-induced granuloma model and adjuvant induced chronic arthritis in rats. The findings of in vitro antioxidant activity confirmed that hydroalcoholic extracts expressed higher antioxidant activity compared to aqueous extract, and hence hydroalcoholic extracts was further selected for the isolation of various fractions. The results of phytochemical study suggest that FCM6, FCM7 and FCM8 exposed the presence of polyphenol and flavonoids. The fraction FCM6, FCM7 and FCM8 (25 mg/kg) exhibits significant antiinflammatory and anti-arthritis activity. The outcomes suggest that the anti-inflammatory and anti-arthritis activity of isolated fraction was due to presence of flavonoids and polyphenols.

A Comprehensive Review of Kshavaka: An Important Medicinal Plant of Ayurveda

“…The chemicals extracted from Centipeda minima containing poly phenols and flavonoids compound and these organic substances impart important role in anti-inflammatory and anti-arthritic activity. 31…”

Qualitative and quantitative analysis of sesquiterpene lactones in Centipeda minima by UPLC–Orbitrap–MS & UPLC-QQQ-MS

“…The use of the whole plant in Chinese folk medicine for treating cold, nasal allergy, diarrhea, malaria and asthma has a long history [2]. Previous pharmacological studies have demonstrated that extracts of Centipeda minima and some isolated compounds from the Centipeda minima possess anti-tumor [3,4], anti-proliferative [5,6], antioxidant and anti-inflammatory [7,8] properties. Phytochemical investigation of this herbal material has led to the identification of various compounds such as sesquiterpene lactones, triterpenes, flavonoids, polyphenol, phenolic and organic acid [8][9][10][11][12].…”

Cytotoxic and Anti-Inflammatory Sesquiterpenes from the Whole Plants of Centipeda minima

“…The plant of Centipeda minima is used commonly as a traditional Chinese medicine for treating colds, nasal allergy, cough, asthma, headaches, nasopharyngeal carcinoma, and swellings. − Earlier phytochemical investigations of Centipeda minima revealed the presence of sesquiterpene lactones, triterpenoids, terpenoid glycosides, phenolic acids, and flavones . Prior studies have demonstrated that Centipeda minima has multiple biological effects, for example, antitumor, ,, antiproliferative, neuroprotective, antiarthritis, , antibacterial, , antioxidant, and anti-inflammatory activities …”