Anti-inflammatory activities of Taxusabietane A isolated from Taxus wallichiana Zucc.

Khan, Inamullah; Nisar, Muhammad; Shah, Muhammad Raza; Shah, Hamid Ullah; Gilani, Syed Naeemuddin; Gul, Farzana; Abdullah, Syed Muhammad; Ismail, Mohammad; Khan, Naeem; Kaleem, Waqar Ahmad; Qayum, Mughal; Khan, Haroon; Obaidullah,; Samiullah, -; Ullah, Majeed

doi:10.1016/j.fitote.2011.06.003

Cited by 52 publications

(40 citation statements)

References 7 publications

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“…They also play a significant role in cancer cell growth, metastasis, invasiveness, cell survival and induction of tumor necrosis factor (TNF) (Khan et al, 2009;. Catalytic or active site of lipoxygenase is composed of 3 important amino acid residues the socalled catalytic triad (His523, His518, Ile875) along with iron atom (Khan et al, 2011). Any compound, which is capable to show strong molecular interaction with the catalytic triad, can be developed as new and therapeutically effective lipoxygenase inhibitor.…”

Section: Resultsmentioning

confidence: 99%

“…Baicalein and tenidap sodium were used as positive controls for lipoxygenase inhibition (Khan et al, 2009;. The IC50 values were calculated as reported earlier (Khan et al, 2011). Viscosine showed significant inhibitory activity against lipoxygenase.…”

Section: Lipoxygenase Inhibition Assaymentioning

confidence: 99%

“…Molecular coordinates were further optimized using MMFF force field. FRED 2.1 (Khan et al, 2011) was used to dock the OMEGA pre-generated multi-conformer library. De-fault FRED protocol was used except for the size of the box defining the binding sites.…”

Section: Molecular Docking Simulationsmentioning

confidence: 99%

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Molecular docking of viscosine as a new lipoxygenase inhibitor isolated from <i>Dodonaea viscosa</i>

Khan

Mohammad

Iqbal

et al. 2012

Bangladesh J Pharmacol

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Section: Resultsmentioning

confidence: 99%

Section: Lipoxygenase Inhibition Assaymentioning

confidence: 99%

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Molecular docking of viscosine as a new lipoxygenase inhibitor isolated from <i>Dodonaea viscosa</i>

Khan

Mohammad

Iqbal

et al. 2012

Bangladesh J Pharmacol

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“…OEDocking 3.0.0 (Santa Fe, NM) [18][19][20][21][22] was used in this study to dock the OMEGA pre-generated multi-conformer library. FRED strategy was used, which exhaustively dock/score all possible positions of each ligand in the binding site.…”

Section: Molecular Docking Simulationsmentioning

confidence: 99%

Protein tyrosine phosphatase 1B inhibitors isolated from Artemisia roxburghiana

Shah

Ishtiaq

Hizbullah

et al. 2015

Journal of Enzyme Inhibition and Medicinal Chemistry

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Artemisia roxburghiana is used in traditional medicine for treating various diseases including diabetes. The present study was designed to evaluate the antidiabetic potential of active constituents by using protein tyrosine phosphatase 1B (PTP1B) as a validated target for management of diabetes. Various compounds were isolated as active principles from the crude methanolic extract of aerial parts of A. roxburghiana. All compounds were screened for PTP1B inhibitory activity. Molecular docking simulations were performed to investigate the mechanism behind PTP1B inhibition of the isolated compound and positive control, ursolic acid. Betulinic acid, betulin and taraxeryl acetate were the active PTP1B principles with IC 50 values 3.49 ± 0.02, 4.17 ± 0.03 and 87.52 ± 0.03 mM, respectively. Molecular docking studies showed significant molecular interactions of the triterpene inhibitors with Gly220, Cys215, Gly218 and Asp48 inside the active site of PTP1B. The antidiabetic activity of A. roxburghiana could be attributed due to PTP1B inhibition by its triterpene constituents, betulin, betulinic acid and taraxeryl acetate. Computational insights of this study revealed that the C-3 and C-17 positions of the compounds needs extensive optimization for the development of new lead compounds.

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“…Himalayan medicinal plants form important constituents of alternative medicinal systems such as Amchi, Ayurveda, Han Chinese, Unani, and other traditional medicine systems that are prevalent in this region. Existing literature on T. wallichiana shows its analgesic, antipyretic, anti-inflammatory, immunomodulatory, antiallergic, anticonvulsant, anticociceptive, antiosteoporotic, antibacterial, antifungal, antiplatelet, and antispasmodic activities, and vasorelaxing effect [2,[4][5][6][7]. In India, its leaves and bark are used in Unani drug "Zarnab" prescribed as a sedative, aphrodisiac and as cure for bronchitis, asthma, epilepsy, snake bite, and scorpion stings [3].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Antioxidant Potential and Hepatoprotective Activity of Taxus Wallichiana

Bhat

Ganie

Dar

et al. 2018

Asian J Pharm Clin Res

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Objectives: Taxus wallichiana, the member of family Taxaceae is used for the cure of various ailments by a large ethnic population native to the forests in Kashmir valley. The aim of this study is to evaluate antioxidant potential and hepatoprotective action of T. wallichiana as claimed by the ethnic population segment.Methods: Antioxidant potential of different T. wallichiana extracts was evaluated by estimating the total phenolics, 1,1-diphenyl -2-picrylhydrazyl (DPPH), reducing power, microsomal lipid peroxidation (LPO), and hydroxyl radical scavenging activity through standard protocols. Hepatoprotective activity of T. wallichiana was determined by evaluation of liver marker enzyme (aspartate transaminase, alanine transaminase, and alkaline phosphatase) levels using standard protocols and by histological analysis of rat liver tissues of different animal groups against CCl 4 induced liver damage.Results: Phenolic content expressed as gallic acid equivalents (GAE/g) was found maximum in case of ethanolic extract (153.34 mg GAE/g), followed by extracts of methanol (149.24 mg GAE/g), hexane (134 mg GAE/g), ethyl acetate (128.9 mg GAE/g), chloroform (121.37 mg GAE/g), and aqueous (113.00 mg GAE/g). At concentration of 700 µg/ml, DPPH radical scavenging activity of methanol extract was 88.29% with IC 50 (212.00 µg/ml), aqueous (82.47%) with IC 50 (258.29 µg/ml), and ethyl acetate (79.57%) with IC 50 (301.80%µg/ml). The reducing power of the extracts increased in a concentration dependent manner. At concentration of 700 µg/ml, 79%, 68%, and 56% inhibition was observed with extracts of methanol, aqueous, and ethyl acetate on microsomal LPO with IC 50 values of 126.09, 168.83, and151.96 µg/ml, respectively. Superoxide radical scavenging activity of T. wallichiana extracts increased in a dose-dependent manner with IC 50 values 170.30 µg/ml (methanol), 257.00 µg/ml (aqueous), and 297.55 µg/ml ethyl acetate extract. T. wallichiana extracts exhibited antioxidant effects on calf thymus DNA damage. The results further depict the hepatoprotective action of T. wallichiana extract at doses of 100 and 300 mg/kg and were comparable with that of standard treatment comprising 50 mg/kg-day of Vitamin C, a known hepatoprotective agent. The data were substantiated with histopathological studies of rat liver sections. Conclusion:These results comprehensively depict that T. wallichiana extracts are endowed with the free radical sequestering potential and be employed as potential antioxidant and hepatoprotective arsenal against many oxidative stress linked diseases.

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Anti-inflammatory activities of Taxusabietane A isolated from Taxus wallichiana Zucc.

Cited by 52 publications

References 7 publications

Molecular docking of viscosine as a new lipoxygenase inhibitor isolated from <i>Dodonaea viscosa</i>

Molecular docking of viscosine as a new lipoxygenase inhibitor isolated from <i>Dodonaea viscosa</i>

Protein tyrosine phosphatase 1B inhibitors isolated from Artemisia roxburghiana

In Vitro Antioxidant Potential and Hepatoprotective Activity of Taxus Wallichiana

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