Anti-hemolytic, Anti-lipid Peroxidation and Antioxidant Properties of Three Plants Locally Used to Treat Metabolic Disorders: Allium sativum, Persea americana and Citrus sinensis

Abstract

Citrus sinensis, Persea americana and Allium sativum are good sources of large number of various bioactive substances including antioxidants. Antioxidants play an essential role in the prevention and treatment of type 2 diabetes and other metabolic diseases. This study was conducted to evaluate and compare the anti-lipid peroxidation, anti-hemolytic and antioxidant capacity of aqueous extracts of the three plants in vitro. Polyphenolic and flavonoids content of aqueous extracts (EA) of plants were determined. The anti-lipid peroxidation was evaluated on homogenates of rat liver. Anti-haemolytic effects were assessed using erythrocyte system model induced free radicals from human red blood cells. Total antioxidant capacity and antiradical or scavenging capacity were evaluated on biological free radical systems (OH, NO . ) and synthetic radicals (DPPH and ABTS + ). Results showed that, different extracts possess variable amounts of polyphenolic compounds. They exhibited significant anti-hemolytic and anti-lipid peroxidation activities. Aqueous extracts of Citrus sinensis are powerful scavenger of OH . (IC 50 =1.05x10 -3 mg/ml ) and NO . (IC 50 = 1.29x10 -3 mg/ml) free radicals while Persea americana seed extract possesses the highest anti-lipid peroxidation capacity (59.72%) and the best scavenging capacity against DPPH and ABTS. Red blood cells were highly protected (at 97.87%, p<0.05) by Allium sativum extract at 1mg/mL and Citrus sinensis (87.7%, p<0.05). Therefore, anti-hemolytic, anti-lipid peroxidation effects of the aqueous extracts of the three plants can justify their use and efficacy in alternative treatment of metabolic diseases.

Total Polyphenol and Flavonoid Content and Antioxidant Capacity of Some Varieties of Persea americana Peels Consumed in Cameroon

“…Free radicals include hydroxyl radicals, peroxyl radicals, superoxide radicals, hydrogen peroxide, singlet oxygen, and various lipid peroxides [ 35 ]. Considering the variety of oxidation processes and reactions that have been reported [ 10 , 36 ], a single method cannot be enough to provide a clear idea of the antioxidant potential of a compound. However, a single test cannot judge the antioxidant activity of an extract [ 37 ].…”

“…Considering the variety of oxidation processes and reactions that have been reported [10,36], a single method cannot be enough to provide a clear idea of the antioxidant potential of a compound. However, a single test cannot judge the antioxidant activity of an extract [37].…”

“…When the pulp is used, the seeds and peels are discarded as wastes, constituting environmental challenges. The aqueous extracts of avocado seeds showed good antioxidant properties useful against lipid peroxidation [ 10 ]. Some studies were carried out on the phenolic content and the antioxidant capacity of peels of three varieties of avocado, namely, Hass, Fuerte, and Shepard [ 7 , 9 ], and they demonstrated that avocado peels could be consumed as functional ingredients.…”

“…Several studies reported that much of the total antioxidant activity is related to the phenolic content of the food products [6][7][8]. Fruits, vegetables, and many other plants have been reported to be rich in phenolic compounds [6,9,10].…”

Phytochemical Screening and Antioxidant Potential of Aqueous Extracts of Millettia laurenti, Lophira alata and Milicia excelsa, Commonly Used in the Cameroonian Pharmacopoeia

“…Considering the variety of oxidation processes and reactions that have been reported [29,30], a single method cannot be enough to provide a clear idea of the antioxidant potential of a compound. In the present study, four different methods were used to evaluate the antioxidant activity of the plant AEs: Two based on the antiradical scavenging activity (DPPH* and NO*) and two others based on the reducing potential (TAC and FRAP).…”