Anti-Cell Proliferative Efficacy of Ferulic Acid Against 7, 12-dimethylbenz(a) Anthracene Induced Hamster Buccal Pouch Carcinogenesis

Abstract

The present study was designed to explore the anti-cell proliferative efficacy of ferulic acid by analysing the expression pattern of cell proliferative markers, proliferating cellular nuclear antigen (PCNA) and cyclin D1, in the buccal mucosa of golden Syrian hamsters treated with 7,12-dimethylbenz(a)anthracene (DMBA). Oral squamous cell carcinomas developed in the buccal pouch of hamsters using topical application of 0.5% DMBA three times a week for 14 weeks. Immunohistochemical (PCNA) and RT-PCR (Cyclin D1) analysis revealed over expression of PCNA and cyclin D1 in the buccal mucosa of hamsters treated with DMBA alone (tumor bearing hamsters). Oral administration of ferulic acid at a dose of 40 mg/kg bw to hamsters treated with DMBA not only completely prevented the tumor formation but also down regulated the expression of PCNA and cyclin D1. The results of the present study thus suggests that ferulic acid might have inhibited tumor formation in the buccal mucosa of hamsters treated with DMBA through its anti-cell proliferative potential as evidenced by decreased expression of PCNA and cyclin D1.Keywords: Oral cancer -hamster buccal cavity -DMBA -ferulic acid -cyclin D1 -PCNA.

Coffee provides a natural multitarget pharmacopeia against the hallmarks of cancer

“…p-coumaric acid up-regulates CDKN1A (cyclin-dependent kinase inhibitor 1), CCNA2 (cyclin A2) and CCNB1 (cyclin B1) (Janicke et al 2011). In an in vivo inducible hamster buccal pouch carcinoma model, the oral administration of ferulic acid prevented cancer formation and down-regulated the expression of PCNA (proliferating cell nuclear antigen) and cyclin D1 (Prabhakar et al 2012). After inducing cell cycle arrest in the G0/G1 phase, chlorogenic acid (Fig.…”

“…In an inducible oral tumor mouse model, ferulic acid (Fig. 1), a phenolic compound released during chlorogenic acid catabolism, decreased the expression of PCNA, an essential processivity factor of DNA replication (Prabhakar et al 2012).…”

Ferulic acid prevents liver injury induced by Diosbulbin B and its mechanism

“…(Ligusticum) (8). FA exerts multiple biological activities such as antioxidant, and anti-inflammation (9,10) and it is known for treatment of Alzheimer's disease (11), cardiovascular disease (12), and cancer (13). In addition, FA is reported to have hepato-protective activity (14,15).…”

Modulating Effect of Ferulic Acid on NF-kB, COX-2 and VEGF Expression Pattern During 7, 12-Dimethylbenz(a)anthracene Induced Oral Carcinogenesis

“…Previous studies from our laboratory have also shown the chemopreventive potential of ferulic acid against oral and mammary carcinogenesis [23,24]. Recently we have shown the proapoptotic and anti-cell proliferative efficacy of ferulic acid against DMBA induced hamster buccal pouch carcinogenesis [25,26]. The present study explores the anticell proliferative efficacy of ferulic acid against 7, 12dimethylbenz(a)anthracene inducing hamsters buccal pouch carcinogenesis.…”