Anti-biofilm activity of plant derived extracts against infectious pathogen-Pseudomonas aeruginosa PAO1

Badrealam, Khan Farheen; Farraj, Dunia A. Al; Mah-e-Fatima, Syeda; Yameen, Muhammad Arfat; Elshikh, Mohamed S.; Alkufeidy, Roua M.; Mustafa, Abd El-Zaher M.A.; Bhasme, Pramod; Alshammari, Maryam K.; Alkubaisi, Noorah A.; Abbasi, Arshad Mehmood; Naqvi, Tatheer Alam

doi:10.1016/j.jiph.2020.07.007

Cited by 53 publications

(28 citation statements)

References 55 publications

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“…Avisible reductions in the biofilm formation were observed. Many researchers have reported the inhibitory effects of different plants on biofilm formation by different bacteria [43][44][45][46][47]. The study of the expression levels of the genes related to the efflux activity in S. enterica isolates.…”

Section: Discussionmentioning

confidence: 99%

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Antidiarrheal and Antibacterial Activities of Monterey Cypress Phytochemicals: In Vivo and In Vitro Approach

et al. 2022

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Monterey cypress (Cupressus macrocarpa) is a decorative plant; however, it possesses various pharmacological activities. Therefore, we explored the phytochemical profile of C. macrocarpa root methanol extract (CRME) for the first time. Moreover, we investigated its antidiarrheal (in vivo), antibacterial, and antibiofilm (in vitro) activities against Salmonella enterica clinical isolates. The LC-ESI-MS/MS analysis of CRME detected the presence of 39 compounds, besides isolation of 2,3,2″,3″-tetrahydro-4′-O-methyl amentoflavone, amentoflavone, and dihydrokaempferol-3-O-α-l-rhamnoside for the first time. Dihydrokaempferol-3-O-α-l-rhamnoside presented the highest antimicrobial activity and the range of values of MICs against S. enterica isolates was from 64 to 256 µg/mL. The antidiarrheal activity of CRME was investigated by induction of diarrhea using castor oil, and exhibited a significant reduction in diarrhea and defecation frequency at all doses, enteropooling (at 400 mg/kg), and gastrointestinal motility (at 200, 400 mg/kg) in mice. The antidiarrheal index of CRME increased in a dose-dependent manner. The effect of CRME on various membrane characters of S. enterica was studied after typing the isolates by ERIC-PCR. Its impact on efflux and its antibiofilm activity were inspected. The biofilm morphology was observed using light and scanning electron microscopes. The effect on efflux activity and biofilm formation was further elucidated using qRT-PCR. A significant increase in inner and outer membrane permeability and a significant decrease in integrity and depolarization (using flow cytometry) were detected with variable percentages. Furthermore, a significant reduction in efflux and biofilm formation was observed. Therefore, CRME could be a promising source for treatment of gastrointestinal tract diseases.

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Section: Discussionmentioning

confidence: 99%

“…This experiment was adopted according to a previously reported protocol [45]. Different groups (n = 6) of mice were abstained from food (but not water) for 18 h. Diarrhea was induced in mice by the oral receipt of 0.5 mL of castor oil, one hour after dosing (as previously mentioned.…”

Section: Diarrhea Induced With Castor Oil Oral Administration In Micementioning

confidence: 99%

Antidiarrheal and Antibacterial Activities of Monterey Cypress Phytochemicals: In Vivo and In Vitro Approach

et al. 2022

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“…The B. gymnorhiza and its associates have been reported to possess various biological properties of therapeutic importance such as antimicrobial, antioxidant, neuroprotective, and so on ( Wu et al, 2015 ; Barik et al, 2013 ). Anti-QS and anti-biofilm compounds such as synthetic AHL analogs ( Moore et al, 2015 ; Shin et al, 2019 ), emodin, patulin from Plectosphaerella cucumerina extract ( Zhou et al, 2017 ), Berginia ciliata ( Soukarieh et al, 2018 ), Clematis viticella ( Alam et al, 2020 ), Origanum vulgare ( Camele et al, 2019 ), and parthenolide ( Lu et al, 2019 ) from natural sources have been reported recently. However, B. gymnorhiza has not been evaluated or reported yet as a source of anti-biofilm and anti–quorum sensing activity against P. aeruginosa.…”

Section: Discussionmentioning

confidence: 99%

A Cyclic Disulfide Diastereomer From Bioactive Fraction of Bruguiera gymnorhiza Shows Anti–Pseudomonas aeruginosa Activity

2022

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Pseudomonas aeruginosa is an opportunistic pathogen that commonly causes hospital-acquired infection and is of great concern in immunocompromised patients. The quorum sensing (QS) mechanism of P. aeruginosa is well studied and known to be responsible for pathogenicity and virulence. The QS inhibitor derived from the natural product can be an important therapeutic agent for pathogen control. The present study reports the role of Bruguiera gymnorhiza purified fraction (BG138) in inhibiting virulence factor production, biofilm formation, quorum sensing molecules, and expression of QS-related genes of P. aeruginosa. Structural characterization of BG138 by high resolution mass spectrometry, Fourier transform infrared spectroscopy, 1D (1H and 13C NMR) and 2D NMR reveals that the fraction is a mixture of already known cyclic disulfide diastereomer, namely, brugierol and isobrugierol. The minimum inhibitory concentration (MIC) of BG138 against P. aeruginosa was 32 μg/ml. Biofilm formation was significantly reduced at sub-MIC concentrations of BG138. Scanning electron microscopy analysis reports the concentration-dependent biofilm inhibition and morphological changes of P. aeruginosa. Flow cytometry–based cell viability assay showed that P. aeruginosa cells exhibit increased propidium iodide uptake on treatment with 32 and 64 μg/ml of BG138. At sub-MIC concentrations, BG138 exhibited significant inhibition of virulence factors and reduced swimming and swarming motility of P. aeruginosa. Furthermore, the effect of BG138 on the expression of QS-related genes was investigated by qRT-PCR. Taken together, our study reports the isolation and structural characterization of bioactive fraction BG138 from B. gymnorhiza and its anti-biofilm, anti-virulence, anti-quorum sensing, and cell-damaging activities against P. aeruginosa.

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“…One of the factors responsible for antibiotic resistance is biofilm formation. In an extracellular matrix, a complex microbial group will be encased which defends itself from the harsh environment and these are known as biofilms (Alam et al, 2020). Since bacterial biofilms guard themselves against antimicrobial agents, they increase their resistance toward antibiotics and environmental stress and pose severe issues in food industries (Hakimi Alni et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antimicrobial and antibiofilm properties of chitosan edible coating with plant extracts against Salmonella and E. coli isolated from chicken

Johnson

Thakur

Etikala

et al. 2022

Food Processing Preservation

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This study aims to investigate the effect of chitosan edible coating with medicinal leaf extracts, their antibiofilm and antimicrobial activity against the selected strains of Salmonella spp. and E. coli isolated from the chicken sample. The presence of bioactive compounds in leaf extracts was confirmed by antioxidant assay, polyphenolic content, UV–VIS, FTIR, and GC–MS analysis. Chitosan with M. piperita and P. amboinicus (ECMO) had a greater zone of inhibition against the multidrug‐resistant strains (Salmonella spp., 22.10 ± 1.15; E. coli, 24.50 ± 0.500) and biofilm inhibition percentage was ≤96% in both Salmonella spp. and E. coli. In shelf‐life analysis, ECMO significantly lowered the growth of pathogens during 15 days of storage period. The results suggest that ECMO was identified as the impeding agent for biofilms and foodborne pathogens. To our knowledge, this is the first study demonstrating the antibiofilm and antibacterial properties of chitosan with M. piperita and P. amboinicus. Novelty impact statement The combination of chitosan with M. piperita and P. amboinicus is the first study on chicken that significantly controlled pathogens during storage period at 4°C. Antibiofilm property was exhibited by chitosan with M. piperita and P. amboinicus against Salmonella spp. and E. coli. Chitosan with M. piperita and P. amboinicus, perchance a new natural preservative for perishable foods.

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Anti-biofilm activity of plant derived extracts against infectious pathogen-Pseudomonas aeruginosa PAO1

Cited by 53 publications

References 55 publications

Antidiarrheal and Antibacterial Activities of Monterey Cypress Phytochemicals: In Vivo and In Vitro Approach

Antidiarrheal and Antibacterial Activities of Monterey Cypress Phytochemicals: In Vivo and In Vitro Approach

A Cyclic Disulfide Diastereomer From Bioactive Fraction of Bruguiera gymnorhiza Shows Anti–Pseudomonas aeruginosa Activity

Evaluation of antimicrobial and antibiofilm properties of chitosan edible coating with plant extracts against Salmonella and E. coli isolated from chicken

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