Anti-bacterial selenium nanoparticles produced by UV/VIS/NIR pulsed nanosecond laser ablation in liquids

Abstract

The ability to produce nanoparticles free of any surface contamination is very challenging especially for bio-medical applications. Using a pulsed nanosecond Nd-YAG laser, pure selenium nanoparticles have been synthesized by irradiating selenium powder (99.999%) immerged in de-ionized water and ethanol. The wavelength of the laser beam has been varied from the UV to NIR (355, 532 and 1064 nm) and its effect on the particle size distribution has been studied by dynamic light scattering (DLS) and transmission electronic microscopy (TEM), revealing then the production of selenium quantum dots (size < 4 nm) by photofragmentation. It has been found that the crystallinity of the nanoparticles depends on their size. The zeta-potential measurement reveals that the colloidal solutions produced in de-ionized water were stable while the ones synthesized in ethanol agglomerate. The concentration of selenium has been measured using inductively coupled plasma mass spectrometry (ICP-MS). The anti-bacterial effect of selenium nanostructures has been analyzed on E. Coli bacteria. Finally, selenium quantum dots produced by this method can also be useful for quantum dot solar cells.

Inhibition of Candida albicans biofilm by pure selenium nanoparticles synthesized by pulsed laser ablation in liquids

“…2. It has already been noted by Guisbiers et al (28) that the crystallinity of the Se NPs synthesized with a nanosecond laser was size-dependent i.e. the largest nanoparticles were amorphous and the smallest ones where crystalline with a transition at room temperature found to be around ~80 nm.…”

“…Indeed, C. albicans form generally a biofilm that protects themselves from the immune system(52). Therefore, the strategy is to apply the pure Se NPs synthesized by PLAL on the biofilm produced by this fungus as selenium is known for its anti-bacterial properties(6, 17, 28). The inhibition was tested following a well-known phenotypic assay(23, 33).…”

Hybrid nanomaterial: biocolloidals

“…One of the widely employed types of nanomaterials with diameters between 1 and 100 nm are iron oxide NPs (IONPs). Much attention was drawn to their significant features, which make IONPs very suitable for potential technologic applications in materials engineering and biological sciences (Griesser et al, 2002;Harris, 2004;Fudouzi and Sawada, 2006;Gioux et al, 2010;Gabudean et al, 2012;Giorgetti et al, 2012;Frank et al, 2013;Garg et al, 2013;Gatto et al, 2013;Hasany et al, 2013;Ghosh and Collie, 2014;Gonella and Dai, 2014;Hashemizadeh and Huyeh, 2014;Hassan and Singh, 2014;Gui et al, 2015;Guisbiers et al, 2015;Harris et al, 2015;Helmbrecht et al, 2015;Garg, 2016). Since they are relatively nontoxic to human organs, magnetite and maghemite are selected to be used in biomedical applications.…”

“…Recent advances for colloidal particles have enabled the synthesis of highly monodispersed colloidal particles with biomolecules to obtain more functional hybrid nanoscale materials. To utilize the unique properties of nanomaterials, these advanced methods have always been examined for a biological setting (Hayward et al, 2000;Joannopoulos et al, 2011;Im et al, 2011;Hasany et al, 2013;Han et al, 2014;Hashemizadeh and Huyeh, 2014;Hassan and Singh, 2014;Kumar et al, 2014;Gui et al, 2015;Guisbiers et al, 2015;Harris et al, 2015;Rodio et al, 2016).…”

Introducing theLaser PhysicsandLaser Physics Lettershighlights of 2015

“…• Anti-bacterial selenium nanoparticles produced by UV/VIS/NIR pulsed nanosecond laser ablation in liquids-using a pulsed nanosecond Nd-YAG laser, pure selenium nanoparticles have been synthesized by irradiating selenium powder (99.999%) immerged in de-ionized water and ethanol [61].…”