Anti-bacterial activity of ethanol extract of the stem of coffee parasite (Scurulla ferruginea (Robx. Ex Jack) Danser) and its secondary metabolite isolation

Abstract

The aim of this study is to determine the antibacterial activity of the ethanol extract of the stem of the coffee parasite (Scurulla ferruginea (Robx. Ex Jack) Danser) using disc diffusion and microdilution methods. In this study, secondary metabolites were isolated from coffee parasite stems using Vacuum Liquid Chromatography and Column Chromatography continued by characterized the compounds using GC-MS (Gas chromatography-mass spectroscopy). Based on the identification using GC-MS, it was known that the stem of the coffee parasite contains diisooctyl phthalate. The results of the antibacterial activity test of the disc diffusion method with an extract concentration of 1% against Staphylococcus aureus, Salmonella typhi, Streptcoccus viridans, Escherichia coli, Streptococcus mutans, and Bacillus aureus bacteria were 6.35 mm, 6.7 mm, 8.95 mm, 7 mm, 8.75 mm and 8.2 mm respectively. This result shows that the ability to inhibit bacterial growth of 1% extract is in the medium category. The MIC values for Escherichia coli, Streptococcus mutans, and Bacillus aureus bacteria were 2500 g/mL, while the MIC values for Staphylococcus aureus, Staphylococcus typhi, Streptcoccus viridans were 5000 g/mL. The MBC value of Streptcoccus viridans, Streptococcus mutans, and Bacillus aureus bacteria was greater than 5000 g/mL, only Escherichia coli bacteria can be killed at a concentration of 5000 g/mL and Salmonella typhi at a concentration of 2500 g/mL. The test results showed that the stem extract of Scurulla ferruginea was only as an inhibitor.