Anti-adhesion activity of thyme (Thymus vulgarisL.) extract, thyme post-distillation waste, and olive (Olea europeaL.) leaf extract againstCampylobacter jejunion polystyrene and intestine epithelial cells

Pogačar, Maja Šikić; Klančnik, Anja; Bučar, Franz; Langerholc, Tomaž; Možina, Sonja Smole

doi:10.1002/jsfa.7391

Cited by 35 publications

(51 citation statements)

References 32 publications

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“…(van Alphen et al, 2012) had similar findings and observed that pre-exposure of C. jejuni to low concentrations of carvacrol modulated motility and invasion of INT-407 intestinal epithelial cells without affecting intracellular ATP levels or epithelial function. Reduced motility and attachment efficiency in C. jejuni on exposure to clove oil (Kovács, 2014), thyme (Pogacar et al, 2016), herbal extracts (Bensch et al, 2011), and campynexins (Johnson et al, 2015) have also been observed.…”

Section: Discussionmentioning

confidence: 98%

Trans-Cinnamaldehyde, Carvacrol, and Eugenol Reduce Campylobacter jejuni Colonization Factors and Expression of Virulence Genes in Vitro

et al. 2017

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Campylobacter jejuni is a major foodborne pathogen that causes severe gastroenteritis in humans characterized by fever, diarrhea, and abdominal cramps. In the human gut, Campylobacter adheres and invades the intestinal epithelium followed by cytolethal distending toxin mediated cell death, and enteritis. Reducing the attachment and invasion of Campylobacter to intestinal epithelium and expression of its virulence factors such as motility and cytolethal distending toxin (CDT) production could potentially reduce infection in humans. This study investigated the efficacy of sub-inhibitory concentrations (SICs, concentration not inhibiting bacterial growth) of three GRAS (generally recognized as safe) status phytochemicals namely trans-cinnamaldehyde (TC; 0.005, 0.01%), carvacrol (CR; 0.001, 0.002%), and eugenol (EG; 0.005, 0.01%) in reducing the attachment, invasion, and translocation of C. jejuni on human intestinal epithelial cells (Caco-2). Additionally, the effect of these phytochemicals on Campylobacter motility and CDT production was studied using standard bioassays and gene expression analysis. All experiments had duplicate samples and were replicated three times on three strains (wild type S-8, NCTC 11168, 81–176) of C. jejuni. Data were analyzed using ANOVA with GraphPad ver. 6. Differences between the means were considered significantly different at P < 0.05. The majority of phytochemical treatments reduced C. jejuni adhesion, invasion, and translocation of Caco-2 cells (P < 0.05). In addition, the phytochemicals reduced pathogen motility and production of CDT in S-8 and NCTC 11168 (P < 0.05). Real-time quantitative PCR revealed that phytochemicals reduced the transcription of select C. jejuni genes critical for infection in humans (P < 0.05). Results suggest that TC, CR, and EG could potentially be used to control C. jejuni infection in humans.

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Section: Discussionmentioning

confidence: 98%

Trans-Cinnamaldehyde, Carvacrol, and Eugenol Reduce Campylobacter jejuni Colonization Factors and Expression of Virulence Genes in Vitro

et al. 2017

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“…Antimicrobial activities are expressed as the MICs, in grams of FDSS per litre of medium, to assess the efficacies of the initial waste material (FDSS) for bioactive extract preparation. The antimicrobial efficacies of FDSS at concentrations equal to the MICs determined in the broth microdilution tests were also analysed using the macrodilution method, and thus determined as the percentage of bacterial growth inhibition after 4, 9 and 24 h …”

Section: Methodsmentioning

confidence: 99%

“…To promote the commercial value for these bioactive plant materials, it is important to define the potential use of these grape skin and seed phenolics, e.g. in terms of: (i) natural preservatives in the food and feed industry to avoid synthetic preservatives; (ii) alternative agents with potential for application to novel infection‐prevention strategies; (iii) nutrition or digestion aids, as they are absorbed and metabolized through normal pathways in humans to a large extent; and (iv) use in cosmetics, where plant extracts can cleanse the skin and promote quicker wound healing …”

Section: Introductionmentioning

confidence: 99%

Polyphenol, antioxidant and antimicrobial potential of six different white and red wine grape processing leftovers

et al. 2016

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FDSS extracts from winery by-products show interesting phenolic profiles that include flavonols, catechins, anthocyanins and hydroxycinnamic acids, with yields influenced by grapevine cultivar and extraction conditions. The antioxidant, antimicrobial and anti-adhesive activities of 50% aqueous ethanol 'Pinot Noir' FDSS extract reveals potential applications in food, pharmaceutical and cosmetic industries for these bioactive residues. © 2016 Society of Chemical Industry.

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“…Therefore, a successful reduction in biolm formation can be a potential strategy for the management of disease progression and elimination of a pathogen. 4,6 In this context, we have assessed the antibiolm property of PJE, 34 baicalein, 35 quercetin 36,37 and chrysin 38 against P. aeruginosa. Flavone compounds were reported to have antibacterial and antibiolm activity against bacteria.…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm activity of Parkia javanica against Pseudomonas aeruginosa: a study with fruit extract

Das

Sandhu

et al. 2017

RSC Adv.

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Parkia javanica is a well-known ethno-botanical plant of the north-east region of India. Ethnic communities of the region use several parts, including fruits, of this plant for the treatment of various ailments like diarrhoea, dysentery, cholera, food poisoning etc. In addition fruits are consumed by the local tribes of the north-east as food supplements. With this background we have performed chemical characterisation, and investigated the antimicrobial and antibiofilm potentiality of ethyl acetate fraction of Parkia javanica fruit extract (PJE) against model biofilm-causing microorganism Pseudomonas aeruginosa. PJE was initially prepared from fruit extract, and assayed by IR and UV spectroscopy and HPLC to confirm the presence of compounds. HPLC and NMR analysis reveals that PJE contains flavone compounds baicalein, quercetin and chrysin. PJE, baicalein, quercetin and chrysin were then tested for antimicrobial and antibiofilm activity against P. aeruginosa. PJE showed very significant antimicrobial activity against P. aeruginosa wherein the minimum inhibitory concentration was found at 180 mg mL À1. Interestingly, the antibiofilm study illustrates that minimum concentration of PJE (30 mg mL À1 ) exhibited maximum activity whereas maximum concentration of PJE (90 mg mL À1 ) exhibited minimum antibiofilm activity. It was also observed that compounds baicalein, quercetin and chrysin separately show lower to moderate antibiofilm activity in comparison to PJE. Molecular docking study indicates that baicalein, quercetin and chrysin have good binding affinity with bacterial quorum sensing and motility associated proteins. Furthermore, we have also observed that in comparison with higher concentration, lower concentration of PJE exhibited better attenuation in swarming motility, secretion of proteases and virulence factors like pyoverdin and pyocyanin. AFM study reveals that aggregates in PJE are smaller in size at low concentrations than at higher concentrations. Observations in the present study suggest that PJE as a whole shows higher antibiofilm activity at low concentration whereas individual compounds have comparatively lower antibiofilm activity. This validates the phytomedicinal significance of Parkia javanica against bacterial biofilms.

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Anti-adhesion activity of thyme (Thymus vulgarisL.) extract, thyme post-distillation waste, and olive (Olea europeaL.) leaf extract againstCampylobacter jejunion polystyrene and intestine epithelial cells

Abstract: Our findings suggest that TE, the agro-food waste material TE-R, and the by-product OE represent sources of bioactive phytochemicals that are effective at low concentrations and can be used as therapeutic agents to prevent bacterial adhesion. © 2015 Society of Chemical Industry.

Cited by 35 publications

References 32 publications

Trans-Cinnamaldehyde, Carvacrol, and Eugenol Reduce Campylobacter jejuni Colonization Factors and Expression of Virulence Genes in Vitro

Trans-Cinnamaldehyde, Carvacrol, and Eugenol Reduce Campylobacter jejuni Colonization Factors and Expression of Virulence Genes in Vitro

Polyphenol, antioxidant and antimicrobial potential of six different white and red wine grape processing leftovers

Antibiofilm activity of Parkia javanica against Pseudomonas aeruginosa: a study with fruit extract

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