Anther wall layers control pollen sugar nutrition inLilium

Clément, Christophe; Audran, Jean‐Claude

doi:10.1007/bf01280246

Cited by 101 publications

(91 citation statements)

References 21 publications

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“…The expression pattern indicates that the transport sugar sucrose initially is cleaved by Nin88 synthesized in the tapetal cell layer, whereas after degradation of the tapetum, the corresponding sucrose-cleaving activity is provided by Nin88 that is synthesized by the developing pollen. Thus, the apoplastic carbohydrate supply pathway for the pollen is controlled both by the surrounding tissues of the anther and by the developing pollen in a sequential manner (21). This study strongly supports the importance of apoplastic cleavage of sucrose by extracellular invertase for supplying carbohydrates to sink tissues and for normal development of plants, which has been suggested by an increasing number of studies in recent years (42)(43)(44)(45).…”

Section: Discussionsupporting

confidence: 65%

“…They are nutrients used to sustain growth as well as signals to influence development in vivo and in vitro (20)(21)(22). Accordingly, different male sterile lines were shown to be characterized by perturbed carbohydrate metabolism (6,(20)(21)(22)(23). Assimilates are produced in photosynthetically active source tissues and transported to photosynthetically less active or inactive sink tissues.…”

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confidence: 99%

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Induction of male sterility in plants by metabolic engineering of the carbohydrate supply

Goetz

Godt

Guivarc’h

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

299

250

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Extracellular invertase mediates phloem unloading via an apoplastic pathway. The gene encoding isoenzyme Nin88 from tobacco was cloned and shown to be characterized by a specific spatial and temporal expression pattern. Tissue-specific antisense repression of Nin88 under control of the corresponding promoter in tobacco results in a block during early stages of pollen development, thus, causing male sterility. This result demonstrates a critical role of extracellular invertase in pollen development and strongly supports the essential function of extracellular sucrose cleavage for supplying carbohydrates to sink tissues via the apoplast. The specific interference with phloem unloading, the sugar status, and metabolic signaling during pollen formation will be a potentially valuable approach to induce male sterility in various crop species for hybrid seed production.

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Section: Discussionsupporting

confidence: 65%

mentioning

confidence: 99%

Induction of male sterility in plants by metabolic engineering of the carbohydrate supply

Goetz

Godt

Guivarc’h

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

299

250

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“…2C) classifying NtSUT3 as a 'late pollen gene' [27] similar to pmtl. NtSUT3 is expressed when pollen grains mature in the thecal fluid of the loculus and take up sugar from this fluid [30]. No data on the exact nature of sugars taken up during pollen maturation are available except for the cloning of hexose transporters in petunia and Arabidopsis [19,20].…”

Section: Discussionmentioning

confidence: 99%

Identification of a pollen‐specific sucrose transporter‐like proteinNtSUT3 from tobacco

Lemoine

Bürkle²,

Barker³

et al. 1999

FEBS Letters

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Pollen cells are symplasmieally isolated during maturation and germination. Pollen therefore needs to take up nutrients via membrane carriers. Physiological measurements on pollen indicate sucrose transport in the pollen tube. A cDNA encoding a pollen-specific sucrose transporter-like protein NtSUT3 was isolated from a tobacco pollen cDNA library. NtSUT3 expression is detected only in pollen and is restricted to late pollen development, pollen germination and pollen tube growth. Altogether these data indicate that pollen is supplied not only with glucose, but also with sucrose through a specific sucrose transporter. The respective contribution of each transport pathway may change during pollen tube growth.

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“…The accumulation and mobilization of sugars, lipids and proteins are common during sporangia development and were demonstrated by microscopy, histochemistry, and biochemical, physiological and molecular assays (Pacini & Viegi 1995;Oliver et al 2005;Lora et al 2012;Konyar et al 2013). Regarding to the sugars, their importance was demonstrated for maturation of the epidermis, endothecium and tapetum (Clément & Audran 1995;Clément et al 1998;Castro & Clément 2007). In this subject, was also demonstrated that the middle layers also act in the access of sugars to the loculus (Clément & Audran 1995).…”

mentioning

confidence: 97%

“…Regarding to the sugars, their importance was demonstrated for maturation of the epidermis, endothecium and tapetum (Clément & Audran 1995;Clément et al 1998;Castro & Clément 2007). In this subject, was also demonstrated that the middle layers also act in the access of sugars to the loculus (Clément & Audran 1995).Besides to supply the metabolic demand, sugars will compose the fibrous thickenings of the endothecium, the primexine and intine, and in some cases, also the exine (Walter & Doyle 1975;Horner & Pearson 1978). When preparing for the developmental events of pollen grains, including the pollination period, sugars can be accumulated as starch, callose, pectins, insoluble polysaccharides, disaccharides or monosaccharides (Pacini 1996;Aouali et al 2001;Clément & Pacini 2001;Pacini et al 2006).…”

mentioning

confidence: 99%

Starch distribution in anthers, microspores and pollen grains in Aechmea recurvata (Klotzsch.) L.B.Sm., Dyckia racinae L.B.Sm. and Tillandsia aeranthos (Loisel.) L.B.Sm. (Bromeliaceae)

et al. 2015

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This paper presents a comparative description of the starch distribution in the anthers, microspores and pollen grains of Aechmea recurvata, Dyckia racinae and Tillandsia aeranthos. Flowers at different stages of development were processed according to plant microtechniques for observation by light microscope. Ten stages of embryological development were used as references for the comparative analysis of starch distribution and dynamics. The structural data showed a greater starch accumulation in the parietal layers and connective of D. racinae. It was observed that in the species studied, starch began to accumulate in microspore mother cell stage. The pollen grains in D. racinae and in T. aeranthos present two amylo genesis-amylolysis cycles, while A. recurvata presents only one. One amylogenesis-amylolysis cycle occurs in the parietal layers and/or connective tissue in all three species. The pollen grains in the three species are dispersed without starch and are characterized as the starchless type. Starch dynamics presents a close relation to the development of sporangia, microspores and pollen grains. It is believed that differences in the starch distribution and accumulation are related to the abiotic factors where the species are found. IntroductionThe effectiveness of the reproductive process in angiosperms depends on the viability of microspores, megaspores, pollen grains and embryo sac, where the dynamics and use of primary metabolites are essential in this process (Bhandari 1984;Delph et al. 1997;Shivanna 2003;Pacini et al. 2006;Konyar et al. 2013). The accumulation and mobilization of sugars, lipids and proteins are common during sporangia development and were demonstrated by microscopy, histochemistry, and biochemical, physiological and molecular assays (Pacini & Viegi 1995;Oliver et al. 2005;Lora et al. 2012;Konyar et al. 2013). Regarding to the sugars, their importance was demonstrated for maturation of the epidermis, endothecium and tapetum (Clément & Audran 1995;Clément et al. 1998;Castro & Clément 2007). In this subject, was also demonstrated that the middle layers also act in the access of sugars to the loculus (Clément & Audran 1995).Besides to supply the metabolic demand, sugars will compose the fibrous thickenings of the endothecium, the primexine and intine, and in some cases, also the exine (Walter & Doyle 1975;Horner & Pearson 1978). When preparing for the developmental events of pollen grains, including the pollination period, sugars can be accumulated as starch, callose, pectins, insoluble polysaccharides, disaccharides or monosaccharides (Pacini 1996;Aouali et al. 2001;Clément & Pacini 2001;Pacini et al. 2006). The starch acts on the metabolism or has a structural function (Pacini 1996). In metabolism, starch can be emphasized as an intermediate stage in the formation of oils in starchlesstype pollen grains (Miki-Hirosige & Nakamura 1983;Baker & Baker 1979). It may be possible for it to convert into sucrose for breathing and osmotic regulation (MikiHirosige & Nakamura 1983), o...

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Anther wall layers control pollen sugar nutrition inLilium

Cited by 101 publications

References 21 publications

Induction of male sterility in plants by metabolic engineering of the carbohydrate supply

Induction of male sterility in plants by metabolic engineering of the carbohydrate supply

Identification of a pollen‐specific sucrose transporter‐like proteinNtSUT3 from tobacco

Starch distribution in anthers, microspores and pollen grains in Aechmea recurvata (Klotzsch.) L.B.Sm., Dyckia racinae L.B.Sm. and Tillandsia aeranthos (Loisel.) L.B.Sm. (Bromeliaceae)

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