Anthelmintic effect of four extracts obtained from Caesalpinia coriaria foliage against the eggs and larvae of Haemonchus contortus

Abstract

To investigate the in vitro anthelmintic efficacy of dividivi (Caesalpinia coriaria), a traditional medicinal plant used in Central America and the northern part of South America, extracts from the foliage of this plant were subjected to the egg hatching test (EHT) and larval exsheathment inhibition test (LEIT), against Haemonchus contortus. Four different extracts were evaluated: acetone-water (AW), methanol-water (MW), acetone-water-dichloromethane (AWD) and methanol-water-dichloromethane (MWD). The concentrations used for the EHT and LEIT tests ranged from 500 to 4000 µg mL-1 and six repetitions per concentration. The effective concentrations (EC50) were calculated using Probit analysis. The EC50 for EHT were 2947.0, 3347.0, 3959.6 and 4538.7 µg mL-1 for MWD, MW, AW and AWD, respectively. The EC50 for LEIT were 2883.4, 5927.4, 9876.3 and 9955.4 µg mL-1 for AWD, AW, MWD and MW, respectively. The methanol extracts were the most effective in inhibiting the hatching of eggs, while the acetone extracts showed efficacy in inhibiting larval exsheathment. This study explains the importance that C. coriaria has as a medicinal plant in Central and South American countries.

Solid-State Fermentation as a Sustainable Tool for Extracting Phenolic Compounds from Cascalote Pods

“…A total condensed tannins content of 7.72 mg/gdw was reported by Pineda-Peña et al [40]. The values for condensed and total polyphenols released from cascalote pods in the present work are 120-521% and 265-288% higher than the values previously reported [38][39][40].…”

“…Using extraction by maceration with methanol, Sánchez et al [38] reported values of 21.71 mg/gdw for condensed tannins and 32.06 mg/gdw for total tannins. Rojas-Morales et al [39] reported values for condensed and total tannins of 13 and 34 mg/gdw, respectively. A total condensed tannins content of 7.72 mg/gdw was reported by Pineda-Peña et al [40].…”

“…Identified polyphenol molecules in cascalote pods have different biological activities, such as antioxidant effects, hepatoprotective effects [41], anthelmintic effects [39,44], arginase inhibitory activity [45], antimicrobial activity [4,43,46], and gastroprotective effects [40]. These benefits provide a wide range of possible applications in the food, pharmaceutical, and cosmetic industries.…”