Anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold in large animal model

Fan, Hongbin; Liu, Haifeng; Toh, S.L.; Goh, James Cho‐Hong

doi:10.1016/j.biomaterials.2009.05.048

Cited by 246 publications

(211 citation statements)

References 33 publications

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“…Mechanical loading of aligned silk scaffolds loaded with BMSCs resulted in increased production of collagen type I, collagen type III and tenascin C, when compared to not aligned and not mechanically stimulated counterparts [380]. Further large animal model studies have demonstrated that silk / BMSCs composites induced functional ACL replacement, with silk degrading in a similar rate as the neotissue was formed [381]. However, complete replacement based on silk did not yield a functional therapy [382], which, in addition to the concerns of allergic reaction, have restricted its use in regenerative medicine [373].…”

Section: Bottom-up Approached For Tendon Repair Based On Natural In Omentioning

confidence: 98%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

179

188

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Section: Bottom-up Approached For Tendon Repair Based On Natural In Omentioning

confidence: 98%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

179

188

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“…Animal models have been long used to study ACL injuries, treatments, and associated complications [6,7,17,19,20,22,26,44,45]. Animal models provide researchers with the ability to perform invasive procedures and to obtain samples at specific times that are not possible in human trials.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, translational work through animal models typically is required to comply with regulatory requirements before proceeding to human trials. Large animal models including pigs, sheep, goats, dogs, and rabbits have been used as surrogates to study the effects of surgical intervention after ACL injury [7,17,19,20,22,26,44]. Among those, the porcine model has been shown to be the closest to the human based on the size and anatomy of the knee [48], functional dependence on the ACL [10], gait biomechanics [60], and similarity of hematology and wound healing characteristics [15,38,40].…”

Section: Introductionmentioning

confidence: 99%

Validation of Porcine Knee as a Sex-specific Model to Study Human Anterior Cruciate Ligament Disorders

Kiapour

Shalvoy

Murray

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Animal models have long been considered an important modality for studying ACL injuries. However, to our knowledge, the value of these preclinical models to study sex-related phenomena associated with ACL injury and recovery has not been evaluated. Questions/purposesWe asked whether (1) prominent anatomic and (2) biomechanical factors differ between female and male porcine knees, particularly those known to increase the risk of ACL injury. Methods Eighteen intact minipig knees (nine males, nine females) underwent MRI to determine the femoral bicondylar width, intercondylar notch size (width, area and index), medial and lateral tibial slope, ACL size (length, cross-sectional area, and volume), and medial compartment tibiofemoral cartilage thickness. AP knee laxity at 30°, 60°, and 90°flexion and ACL tensile structural properties were measured using custom-designed loading fixtures in a universal tensile testing apparatus. Comparisons between males and females were performed for all anatomic and biomechanical measures. The findings then were compared with published data from human knees. Results Female pigs had smaller bicondylar widths (2.9 mm, ratio = 0.93, effect size = À1.5) and intercondylar notches (width: 2.0 mm, ratio = 0.79, effect size = À2.8; area: 30.8 mm 2 , ratio = 0.76, effect size = 2.1; index: 0.4, ratio = 0.84, effect size = À2.0), steeper lateral tibial slope (4.3°, ratio = 1.13, effect size = 1.1), smaller ACL (length: 2.7 mm, ratio = 0.91, effect size = 1.1; area: 6.8 mm 2 , ratio = 0.74, effect size = À1.5; volume: 266.2 mm 3 , ratio = 0.68, effect size = À1.5), thinner medial femoral cartilage (0.4 mm, ratio = 0.8, effect size = À1.1), lower ACL yield load (275 N, ratio = 0.81, effect size = À1.1), and greater AP knee laxity at 30°( 0.7 mm, ratio = 1.32, effect size = 1.1) and 90°(0.5 mm, ratio = 1.24, effect size = 1

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“…In the past decade, promising results have been achieved using mesenchymal stem cells (MSCs) in tendon/ligament engineering. [16][17][18][19][20][21][22][23] However, the potential for MSCs to also differentiate into osteoblastic cells leading to ectopic bone formation raises concerns for their application in tendon repair. Likewise, bone marrow-derived MSCs that have been used in various studies [24][25][26][27][28] also pose a potential risk of ectopic bone formation.…”

Section: Introductionmentioning

confidence: 99%

“…In several in vivo studies, silk fibroin-based engineered ligaments have been proven their ability to restore the function of injured ligament. 16,17,26 Another option is the decellularised tendon/ ligament construct. Although the mechanical and biological properties are a better match to native tissue than any other currently available scaffolds, donor cells may remain in the allograft, even with strict sterilization and cleaning, and thus they can potentially cause inflammatory responses.…”

Section: Introductionmentioning

confidence: 99%

Bioreactor Design for Tendon/Ligament Engineering

Wang

Gardiner

Lin

et al. 2013

Tissue Engineering Part B: Reviews

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Tendon and ligament injury is a worldwide health problem, but the treatment options remain limited. Tendon and ligament engineering might provide an alternative tissue source for the surgical replacement of injured tendon. A bioreactor provides a controllable environment enabling the systematic study of specific biological, biochemical, and biomechanical requirements to design and manufacture engineered tendon/ligament tissue. Furthermore, the tendon/ligament bioreactor system can provide a suitable culture environment, which mimics the dynamics of the in vivo environment for tendon/ligament maturation. For clinical settings, bioreactors also have the advantages of less-contamination risk, high reproducibility of cell propagation by minimizing manual operation, and a consistent end product. In this review, we identify the key components, design preferences, and criteria that are required for the development of an ideal bioreactor for engineering tendons and ligaments.

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Anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold in large animal model

Cited by 246 publications

References 33 publications

The past, present and future in scaffold-based tendon treatments

The past, present and future in scaffold-based tendon treatments

Validation of Porcine Knee as a Sex-specific Model to Study Human Anterior Cruciate Ligament Disorders

Bioreactor Design for Tendon/Ligament Engineering

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