Annexin5 Is Essential for Pollen Development in Arabidopsis

Zhu, Jingen; Yuan, Shunjie; Guo, Wenfei; Qian, Dong; Wu, Xianghua; Jia, Honglei; Gui, Mengyuan; Liu, Wenzhe; An, Lizhe; Xiang, Yun

doi:10.1093/mp/sst171

Cited by 30 publications

(40 citation statements)

References 10 publications

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“…Using RT-PCR and fluorescence intensity analyses, representative overexpression lines undergoing similar levels of transcription of Ann5 were chosen for further analysis (Figures 6B to D). Both Ann5G26EG28E and Ann5G257EG259E were evenly distributed in the cytosol of the pollen tube, which was similar to that of Ann5-GFP or GFP alone (Figure 6E) [42].…”

Section: Resultssupporting

confidence: 56%

“…Ann5 is abundant in mature pollen grains and elongating pollen tubes; however, it was difficult to investigate the function of Ann5 during pollen germination and pollen tube growth using the RNAi lines, as Ann5 suppression resulted in the abortion of pollen grains before the mature pollen stage [42]. Thus, to further understand the in vivo function of Ann5 , Ann5 ectopic overexpression lines were generated by expressing Lat52-Ann5-GFP or Ann5Pro-Ann5 constructs in a wild-type background.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous data demonstrated that Ann5 is predominantly expressed in pollen after the bicellular stage and pollen tube formation. In addition, downregulation of the function of Ann5 in transgenic RNAi lines led to severely sterile pollen grains at the bicellular stage of pollen development [42]. However, the mechanisms underlying the function of Ann5 in pollen remain largely unclear.…”

Section: Introductionmentioning

confidence: 99%

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Annexin5 Plays a Vital Role in Arabidopsis Pollen Development via Ca2+-Dependent Membrane Trafficking

Zhu

Yuan

et al. 2014

PLoS ONE

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The regulation of pollen development and pollen tube growth is a complicated biological process that is crucial for sexual reproduction in flowering plants. Annexins are widely distributed from protists to higher eukaryotes and play multiple roles in numerous cellular events by acting as a putative “linker” between Ca2+ signaling, the actin cytoskeleton and the membrane, which are required for pollen development and pollen tube growth. Our recent report suggested that downregulation of the function of Arabidopsis annexin 5 (Ann5) in transgenic Ann5-RNAi lines caused severely sterile pollen grains. However, little is known about the underlying mechanisms of the function of Ann5 in pollen. This study demonstrated that Ann5 associates with phospholipid membrane and this association is stimulated by Ca2+ in vitro. Brefeldin A (BFA) interferes with endomembrane trafficking and inhibits pollen germination and pollen tube growth. Both pollen germination and pollen tube growth of Ann5-overexpressing plants showed increased resistance to BFA treatment, and this effect was regulated by calcium. Overexpression of Ann5 promoted Ca2+-dependent cytoplasmic streaming in pollen tubes in vivo in response to BFA. Lactrunculin (LatB) significantly prohibited pollen germination and tube growth by binding with high affinity to monomeric actin and preferentially targeting dynamic actin filament arrays and preventing actin polymerization. Overexpression of Ann5 did not affect pollen germination or pollen tube growth in response to LatB compared with wild-type, although Ann5 interacts with actin filaments in a manner similar to some animal annexins. In addition, the sterile pollen phenotype could be only partially rescued by Ann5 mutants at Ca2+-binding sites when compared to the complete recovery by wild-type Ann5. These data demonstrated that Ann5 is involved in pollen development, germination and pollen tube growth through the promotion of endomembrane trafficking modulated by calcium. Our results provide reliable molecular mechanisms that underlie the function of Ann5 in pollen.

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Section: Resultssupporting

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Annexin5 Plays a Vital Role in Arabidopsis Pollen Development via Ca2+-Dependent Membrane Trafficking

Zhu

Yuan

et al. 2014

PLoS ONE

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“…Utilizing a chimera strategy by introducing the pore of plant AtGLR1.1 and AtGLR1.4 into the animal GluR1 channel demonstrated low Ca 2+ permeability and low rectification with nonselective cation pores (Tapken and Hollmann, 2008). Other Ca 2+ -permeable channels are putatively active in pollen on the basis of transcriptomics/proteomics, namely the annexins (Lee et al, 2004;Zhu et al, 2014) and the mechanosensitive channel AtOSCA1 .…”

Section: Ion Fluxes and Gradients At The Tip: An Electrifying Affair?mentioning

confidence: 99%

Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes

et al. 2016

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“…The cml24 mutant displayed delayed pollen germination and inhibited pollen tube elongation, resulting in decreased seed set [69]. Other Ca 2+ binding proteins, like calreticulin [71] and annexin [72,73], participated in pollen development as well. Surprisingly, all these proteins were significantly up-regulated in MT plants ( Table 4), suggesting that Ca 2+ homeostasis was disturbed during MT pollen development.…”

Section: Deps Related To Ca 2+ Bindingmentioning

confidence: 99%

iTRAQ-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (Gossypium hirsutum L.)

Pang

Wei

et al. 2015

Journal of Proteomics

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Annexin5 Is Essential for Pollen Development in Arabidopsis

Cited by 30 publications

References 10 publications

Annexin5 Plays a Vital Role in Arabidopsis Pollen Development via Ca2+-Dependent Membrane Trafficking

Annexin5 Plays a Vital Role in Arabidopsis Pollen Development via Ca2+-Dependent Membrane Trafficking

Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes

iTRAQ-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (Gossypium hirsutum L.)

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