Angiogenic response induced by acellular brain scaffolds grafted onto the chick embryo chorioallantoic membrane

Ribatti, Doménico; Conconi, Maria Teresa; Baiguera, Silvia; Corsi, Patrizia; Parnigotto, Pier Paolo; Nussdorfer, Gastone G.

doi:10.1016/s0006-8993(03)03225-6

Cited by 61 publications

(49 citation statements)

References 21 publications

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“…It is conceivable that extraction techniques may not remove all the factors that impact on angiogenesis. In fact, as demonstrated by immunohistochemical staining, the angiogenic activity may also be dependent on the presence of FGF-2 and TGF-␤, as already demonstrated in other experimental conditions (Taipale et al, 1996;Ribatti et al, 2003). Moreover, the main components of the extracellular matrix, such as the fibrous component, consisting of collagen and elastic fibers and the interfibrillary matrix, which contains mainly proteoglycans, solute, and water, may be involved in the angiogenic response.…”

Section: Discussionsupporting

confidence: 52%

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Angiogenic response induced by acellular aortic matrix in vivo

et al. 2004

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In this study, we investigated the angiogenic response induced by acellular aortic matrices implanted in vivo onto the chick embryo chorioallantoic membrane (CAM), a useful model for such investigation. Results showed that acellular matrices were able to induce a strong angiogenic response comparable to that of fibroblast growth factor 2 (FGF-2), a wellknown angiogenic cytokine. The angiogenic response was further increased when exogenous FGF-2 or transforming growth factor beta 1 (TGF-␤1) were added to the matrices and inhibited by the addition of an anti-FGF-2 or anti-TGF-␤1 antibodies. The response may be considered dependent on a direct angiogenic effect exerted by the matrices and in part also by the presence of FGF-2 and TGF-␤1 in the acellular matrices.

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Section: Discussionsupporting

confidence: 52%

“…In this study, we investigated the angiogenic response induced by acellular aortic matrices implanted in vivo onto the chick embryo chorioallantoic membrane (CAM), a useful model for studying angiogenesis and antiangiogenesis (Ribatti et al, 2001) and the effects of other acellular scaffolds, such as those obtained form brain (Ribatti et al, 2003).…”

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confidence: 99%

Angiogenic response induced by acellular aortic matrix in vivo

et al. 2004

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“…Some reports have shown that nerves made myelin-free by extraction have very low immunogenicity, which supports regeneration and leads to functional recovery. 6,14 Ribatti D et al 20 successfully prepared acellular brain scaffolds using chemical extraction and found that acellular brain scaffolds induce a strong angiogenic response.…”

Section: Discussionmentioning

confidence: 99%

Preparation of the acellular scaffold of the spinal cord and the study of biocompatibility

et al. 2010

Spinal Cord

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Methods: The morphology of the acellular segments was revealed by scanning electron microscopy, immunohistochemistry, and hematoxylin and eosin stain. Biocompatibility was studied by immunohistochemistry. Results: Results show that in spinal cord scaffolds, cells, myelin sheath and axon of nerve fibers were eliminated, and three-dimensional supports of extracellular matrix were reserved. The component analytical results of the acellular spinal cord indicate that they contain laminin, fibronectin and collagen, which can facilitate and induce the regeneration of injured nerves, and enhance the adhesion and proliferation of cells. The acellular spinal cord has a three-dimensional structure and excellent biocompatibility. Conclusion: Our data indicate that acellular spinal cord has certain biological properties and it may be a potential alternative scaffold for spinal cord tissue engineering.

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“…Herein, we demonstrated that the bioengineered matrix results to have native biological and biomechanical characteristics. Moreover, starting from previous studies which reported that acellular matrices maintained the expression of angiogenic proteins (such as bFGF and TGF-b) and exerted strong angiogenic responses [5,7,15,16] the expression of such proteins and the in vitro and in vivo angiogenic response induced by human bioengineered trachea were investigated.…”

Section: Introductionmentioning

confidence: 99%