Aneuploidy induction in human fibroblasts: comparison with results in Syrian hamster fibroblasts

Tsutsui, Takeki; Suzuki, Nobuko; Maizumi, Heiji; Barrett, J. Carl

doi:10.1016/0165-1218(90)90074-c

Cited by 31 publications

(6 citation statements)

References 39 publications

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“…Aneugenic effects imply chromosome lagging during mitosis which leads to aneuploidy. Our results of aneugenic effects are in accordance with the findings which demonstrate the induction of aneuploidies in human and mouse fibroblasts (Tsutsui et al, 1990). It is conceivable that oestadiol induces aneuploidy through mitotic arrest, rather than inhibition of microtubule assembly in vitro (Wheeler et al, 1987).…”

Section: Resultssupporting

confidence: 92%

Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro

Đelić¹,

Spremo-Potparević²,

Marković³

et al. 2006

Acta vet. (Beogr.)

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Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level

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Section: Resultssupporting

confidence: 92%

Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro

Đelić¹,

Spremo-Potparević²,

Marković³

et al. 2006

Acta vet. (Beogr.)

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show abstract

“…The percentage of heteroploid cells with a tetraploid or a near-tetraploid chromosome number was not increased significantly following treatment with BP-A even at 200 µM. High levels of polyploidy induction have been demonstrated in SHE cells treated with DES or E 2 at doses that cause growth arrest (Tsutsui et al, 1983(Tsutsui et al, , 1987(Tsutsui et al, , 1990. Aneuploidy induction in the near-diploid range also has been observed in SHE cells treated with DES or E 2 (Tsutsui et al, 1983(Tsutsui et al, , 1987.…”

Section: Figure 1 -Structures Of Bisphenol-a (A) and Diethylstilbestrmentioning

confidence: 84%

Bisphenol-A induces cellular transformation, aneuploidy and DNA adduct formation in cultured Syrian hamster embryo cells

et al. 1998

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“…Both of these types of DNA lesions are capable of inducing mutations. Some earlier studies showed that DES and E 2 can increase mutations leading to ouabain resistance (137). Experiments demonstrate that DES quinone increases homologous recombination in Escherichia coli (138).…”

Section: Direct Evidence Of Gene Mutations Induced By Estrogensmentioning

confidence: 99%

Chapter 4: Estrogens as Endogenous Genotoxic Agents--DNA Adducts and Mutations

Cavalieri¹,

Frenkel²,

Liehr³

et al. 2000

JNCI Monographs

522

412

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Aneuploidy induction in human fibroblasts: comparison with results in Syrian hamster fibroblasts

Cited by 31 publications

References 39 publications

Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro

Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro

Bisphenol-A induces cellular transformation, aneuploidy and DNA adduct formation in cultured Syrian hamster embryo cells

Chapter 4: Estrogens as Endogenous Genotoxic Agents--DNA Adducts and Mutations

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