Anethum graveolens (dill) – A medicinal herb induces apoptosis and cell cycle arrest in HepG2 cell line

Mohammed, Furkhan Ahmed; Elkady, Ayman I.; Syed, Fareeduddin Quadri; Mirza, Muqtadir Baig; Hakeem, Khalid Rehman; Alkarim, Saleh

doi:10.1016/j.jep.2018.03.008

Cited by 33 publications

(16 citation statements)

References 31 publications

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“…Cell cytotoxicity was performed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay 34 . As the confluence of the cells in the flask cell reached 80%, the medium was discarded, and the cells were washed with 1× phosphate buffer saline (PBS) three times.…”

Section: Methodsmentioning

confidence: 99%

Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions

Najm

Azfaralariff

Dyari

et al. 2021

Sci Rep

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Previous study has shown the antimicrobial activities of mucus protein extracted from Anabastestudineus. In this study, we are interested in characterizing the anticancer activity of the A.testudineus antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacterial activity testing to confirm the fish's AMPs production. The cytotoxic activity of each fraction was also identified. Fraction 2 (F2), which shows toxicity against MCF7 and MDA-MB-231 were sent for peptide sequencing to identify the bioactive peptide. The two peptides were then synthetically produced and subjected to cytotoxic assay to prove their efficacy against cancer cell lines. The IC50 for AtMP1 against MCF7 and MDA-MB-231 were 8.25 ± 0.14 μg/ml and 9.35 ± 0.25 μg/ml respectively, while for AtMP2 it is 5.89 ± 0.14 μg/ml and 6.97 ± 0.24 μg/ml respectively. AtMP1 and AtMP2 treatment for 48 h induced breast cancer cell cycle arrest and apoptosis by upregulating the p53, which lead to upregulate pro-apoptotic BAX gene and downregulate the anti-apoptotic BCL-2 gene, consequently, trigger the activation of the caspase-3. This interaction was supported by docking analysis (QuickDBD, HPEPDOCK, and ZDOCK) and immunoprecipitation. This study provided new prospects in the development of highly effective and selective cancer therapeutics based on antimicrobial peptides.

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Section: Methodsmentioning

confidence: 99%

Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions

Najm

Azfaralariff

Dyari

et al. 2021

Sci Rep

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“…Hydrochloric extracts of dill fruit were used as a traditional medicine for treating gastrointestinal disorders. Seeds used for the treatment of jaundice in liver, spleen, and rheumatism disorders and other inflammatory gout diseases [42]. Apium graveolens L.…”

Section: Discussionmentioning

confidence: 99%

Biologically Active Substance Content in Edible Plants of Zakarpattia and Their Elemental Composition Model

Meleshko

Rukavchuk

Buhyna

et al. 2020

Biol Trace Elem Res

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Consumption of edible plants satisfies a significant part of human body needs in macro-and micronutrients while biologically active substances contain strong antioxidant properties and reduce the risk of a number of diseases. Balanced nutrition and design of personalized diets and treatment rely on the data on the content of macro-and micronutrients and biologically active substances. We determined polyphenol and anthocyanin content in 22 species of local edible plants using modified spectrophotometric method with Folin-Ciocalteu reagent as well as chemical elements' content in a mixture of edible plants from 13 regions using standard procedures. We performed correlational analysis of the obtained data and analysis of the main components in OriginLab, developed regional models of chemical elements' content for a mixture of edible plants, and conducted cluster analysis using common tools in Python. The results of biologically active substances' study demonstrated that the highest content of polyphenolic compounds and anthocyanins was found in grape meal of Vitis vinifera L. The study of chemical elements' content demonstrated that edible plants from lowland areas are the best and revealed clear dependences of the elements on each other and geographical conditions. The analysis of the principal components confirmed this finding. Based on the obtained data, a number of regional models of chemical elements' content in a mixture of edible plants were built, tested, and evaluated. Obtained results are the basis for designing various diets, filling composite databases of the region's food, and creating the newest biologics-pharmabiotics.

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“…The plant is used with calcium; calcium oxalate crystals can accumulate in the kidney and gallbladder (43). Anethum graveolens L. / Dere Otu Suppressed the proliferation (44). No side effects reported.…”

Section: Rheum Palmatum L / Işgınmentioning

confidence: 94%

A Survey Study on Medicinal Plants and Mushrooms Use in Treatment of Cancer by Patients at The Istanbul University Institute of Oncology

Tuncay¹,

Ekici²,

Teke³

et al. 2020

JARHS

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Objective: Medicinal herbs are commonly used as complementary or prophylaxis in common illness, as well as in diseases such as cancer, where the disease is severe. In order to determine the use of plants and mushrooms among cancer patients, a survey was conducted with 100 cancer patients who are receiving treatment at polyclinics at the Istanbul University Institute of Oncology. Material and Methods: The questionnaires were conducted face to face with the patients and the results were evaluated in computer. Results: According to the survey results, only 24 of 100 patients were found to use the plants and mushrooms for treatment, to reduce the side effects of the treatment or to feel better. As a result, it was determined that a total of 17 different plants and 1 mushroom were used. The mechanism of action, side effects, and warnings of these plants and mushrooms on cancer treatment have been investigated. With this study, it was determined how the patients obtained the plants and mushrooms, how the age, gender or socioeconomic status affect the plants and mushrooms used and what kind of feedback the patients give about their experience. Conclusion: The fact that the plants and mushrooms are not supplied by pharmacies, the application of herbal treatments with the advice of people under the title "expert" and "reliable" cause many problems. The survey study yielded promising results about the use of plants and mushrooms by cancer patients in Turkey. Similar studies are expected to be conducted in more clinics.

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Anethum graveolens (dill) – A medicinal herb induces apoptosis and cell cycle arrest in HepG2 cell line

Cited by 33 publications

References 31 publications

Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions

Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions

Biologically Active Substance Content in Edible Plants of Zakarpattia and Their Elemental Composition Model

A Survey Study on Medicinal Plants and Mushrooms Use in Treatment of Cancer by Patients at The Istanbul University Institute of Oncology

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