Anesthetic Considerations for Patients with Bleeding Disorders

Beirne, O. Ross

doi:10.1002/9781119053231.ch14

Cited by 1 publication

(1 citation statement)

References 24 publications

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“…EuP-82 can induce platelet aggregation but not hinder the human blood coagulation system. Therefore, the enzyme may be applicable to wound-healing material, which needs clot stability in the first process [48]. In addition, the enzyme has no thrombin-like activity and is safe for living cells.…”

Section: Figmentioning

confidence: 99%

Characterization of the binding of a glycosylated serine protease from Euphorbia cf. lactea latex to human fibrinogen

Siritapetawee

Talabnin

Vanichtanankul

et al. 2017

Biotech and App Biochem

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In this study, the binding of a glycosylated serine protease (EuP-82) with human fibrinogen was investigated by isothermal titration calorimetry (ITC). ITC analysis indicated that the binding of EuP-82 to fibrinogen in the conditions with or without the activator (Ca ) was an exothermic reaction (dominant negative enthalpy), which tended to be driven by hydrogen bonding and van der Waals interactions. In contrast, the binding of fibrinogen-EuP-82 in the condition with the inhibitor (Zn ) was an unfavorable endothermic reaction. EuP-82 could not inhibit the platelet activity in citrated whole blood via the ADP-receptor pathways (mainly, P2Y1 and P2Y12), but it could enhance the platelet aggregation. The ITC together with whole blood platelet aggregation suggested that EuP-82 provided multiple fibrinogen-binding sites that were not related to the arginine-glycine-aspartate (RGD) and the dodecapeptide sequences of fibrinogen. In addition, EuP-82 had neither thrombin-like activity nor anticoagulant activity. The SR-FTIR spectra revealed that EuP-82 was a glycoprotein. Deglycosylation of EuP-82 did not affect its proteolytic activity. Moreover, EuP-82 did not exhibit any toxicity to the living cells (NIH-3T3). This study supports that EuP-82 may be useful for wound-healing material through stabilizing the clot via the platelet induction for the first process.

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Section: Figmentioning

confidence: 99%