Androgen receptors in rat testis

Hansson, V.; McLean, W. S.; Smith, Albert A.; Tindall, Donald J.; Weddington, S. C.; Nayfeh, Shihadeh N.; French, Frank S.; Ritzén, E. M.

doi:10.1016/0039-128x(74)90169-x

Cited by 35 publications

(12 citation statements)

References 20 publications

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“…The second method of polyacrylamide gel electrophoresis (Hansson et al, 1974) showed no oestrogen-binding components in porcine follicular fluid on Days 17 or 21 comparable to intracellular receptor proteins. The method was functional because androgen receptors were detected in the castrated rat prostatic cytosol, Text- fig.…”

Section: Resultsmentioning

confidence: 89%

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Steroid-binding proteins in follicular fluid and peripheral plasma from pigs, cows and sheep

Cook¹,

Hunter²,

Kelly³

1977

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No unusual steroid-binding proteins that might react with the oocyte or its investments could be detected in follicular fluid. Corticosteroid-binding globulin occurred in follicular fluid from pigs, sheep and cows, and sex hormone-binding globulin occurred in follicular fluid from sheep and cows. The bulk of the steroid in follicular fluid is bound to albumin with low affinity, indicating that steroid molecules can readily be released, and oestrogen can react with the oocyte and granulosa cells in a manner analogous to that demonstrated for target cells bathed with interstitial fluid. Pigs lack a sex-hormone binding globulin in blood plasma and, hence, in follicular fluid. Because no proteins exist in follicular fluid that would compete with antibodies to bind steroids, direct radioimmunoassay of follicular steroids appears to be a valid technique.

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Section: Resultsmentioning

confidence: 89%

“…A small number of porcine follicular fluid samples was examined for the presence of oestrogen receptor-like proteins using 3-25% polyacrylamide gels as described by Hansson et al (1974).…”

Section: Polyacrylamide-gel Electrophoresis (For Receptors)mentioning

confidence: 99%

Steroid-binding proteins in follicular fluid and peripheral plasma from pigs, cows and sheep

Cook¹,

Hunter²,

Kelly³

1977

Reproduction

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“…Binding to the receptor is believed to be an essential initial step in androgen action on the target cell. It has now been shown that seminiferous tubules from the rat testis contain a receptor protein similar to the androgen receptors in other target tissues (Hansson et al, 1973c(Hansson et al, , 1974bMcLean et al, 1974;Smith et al, 1975).…”

Section: Androgen Receptorsmentioning

confidence: 99%

“…In subsequent studies, the testicular androgen receptor has been shown to have other characteristics similar to those of the other target organs (Table 1). It sediments as a 4S component in 0-4 M-KC1 and as a 7-10S component at low salt concentrations (Hansson et al, 1973c(Hansson et al, , 1974bMcLean et al, 1974). The receptors have Stokes' radii greater than 80 A and molecular weights greater than 200,000, as determined by gel filtration on Sephadex G-200 and sucrose-gradient centrifugation (McLean et al, 1975;Hansson et al, 1974b Text- fig.…”

Section: Androgen Receptorsmentioning

confidence: 99%

“…(1) Semini¬ ferous tubules contain intracellular receptors for androgens similar to the receptors in accepted androgen target organs (Hansson et al, 1973c(Hansson et al, , 1974bMcLean et al, 1974;Smith et al, 1975), indicating that the tubules also contain androgen-responsive target cells. (2) FSH has been shown to stimulate the production of an androgen-binding protein (ABP) (Ritzén et al, 1972a;Vernon et al, 1972Vernon et al, , 1973Vernon et al, , 1974Hansson et al, 1973dHansson et al, , 1974c), which is probably secreted by the Sertoli cells (Hagenäs et al, 1974;Vernon et al, 1974).…”

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Regulation of Seminiferous Tubular Function by FSH and Androgen

Hansson¹,

Weddington²,

McLean³

et al. 1975

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Seminiferous tubules contain a cytoplasmic androgen receptor similar to the receptors in the epididymis and ventral prostate. The presence of a cytoplasmic receptor indicates that androgens maintain spermatogenesis by a direct action on certain types of cells within the seminiferous tubule. The Sertoli cell appears to be one of the cell types containing androgen receptors and the receptor might also be present in spermatogonia, primary spermatocytes, or peritubular cells. The Sertoli cell is stimulated by FSH to produce an androgen-binding protein which may serve to increase the accumulation of androgen in the seminiferous epithelium and make it available for binding by intracellular androgen receptors. This may be a way in which FSH enhances the action of androgen on spermatogenesis. Androgens act on the Sertoli cell to increase its response to FSH. This action of androgens on the Sertoli cell results in increased production of androgen-binding protein and may enhance the production of other substances which exert trophic effects on spermatogenesis.

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