Androgen receptor activities of p,p′-DDE, fenvalerate and phoxim detected by androgen receptor reporter gene assay

Xu, Li‐Chun; Sun, Hong; Chen, Jianfeng; Bian, Qian; Lee, Song; Wang, Xinru

doi:10.1016/j.toxlet.2005.06.016

Cited by 55 publications

(25 citation statements)

References 36 publications

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“…Many in vivo studies suggested that pyrethroids may be hormonally active and therefore adversely affect semen parameters (14,18,31). As shown in previous studies, we found that some pyrethroids or their metabolites, including 3-PBA, could bind to estrogen receptors and show some estrogen activities (13), as well as antiandrogenic activities (17,35). Certain chemicals can readily penetrate the blood-testis barrier and directly affect spermatogenesis and functional defects of spermatozoa may be an important factor in male infertility.…”

Section: Discussionsupporting

confidence: 74%

See 1 more Smart Citation

The relation between urinary metabolite of pyrethroid insecticides and semen quality in humans

Xia

Yan

et al. 2008

Fertility and Sterility

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Section: Discussionsupporting

confidence: 74%

“…Their reproductive and endocrine toxicities have been extensively investigated in recent years (12)(13)(14)(15)(16)(17). Animal and limited human studies also suggested possible associations of pyrethroid exposure with semen quality (18)(19)(20)(21).…”

mentioning

confidence: 99%

The relation between urinary metabolite of pyrethroid insecticides and semen quality in humans

Xia

Yan

et al. 2008

Fertility and Sterility

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“…No associations with this compound were observed in the casecontrol studies conducted in Washington State (Biggs et al 2008) and Sweden (Hardell et al 2003), although the latter study, with its small size, was underpowered to detect associations of moderate magnitude. Research suggests that high exposure to p,p´-DDE may increase TGCT risk through the induction of anti androgenic effects, as this compound has been shown to be a competitive antagonist for the androgen receptor (Danzo 1997;Kelce et al 1995;Xu et al 2006). Considerable interest has focused on the possible health effects of PCBs, a class of OC compounds used widely in the manufacturing of electrical equipment and other industrial applications until the institution of bans in the 1970s (banned in Norway in 1980).…”

Section: Discussionmentioning

confidence: 99%

Pre-Diagnostic Serum Concentrations of Organochlorine Compounds and Risk of Testicular Germ Cell Tumors

Purdue¹,

Engel²,

Langseth³

et al. 2009

Epidemiology

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bacKgrOunD: Recent findings suggest that exposure to organochlorine (OC) compounds, chlordanes and p,p´-dichlorodiphenyldichloroethylene (p,p´-DDE) in particular, may increase the risk of developing testicular germ cell tumors (TGCTs). Objective: To further investigate this question, we conducted a nested case-control study of TGCTs within the Norwegian Janus Serum Bank cohort. MethODs: The study was conducted among individuals with serum collected between 1972 and 1978. TGCT cases diagnosed through 1999 (n = 49; 27-62 years of age at diagnosis) were identified through linkage to the Norwegian Cancer Registry. Controls (n =51) were matched to cases on region, blood draw year, and age at blood draw. Measurements of 11 OC insecticide compounds and 34 polychlorinated biphenyl (PCB) congeners were performed using gas chromatography/highreso lu tion mass spectrometry. Case-control comparisons of lipid-adjusted analyte concentrations were performed using the Wilcoxon signed-rank test. Odds ratios (ORs) and 95% confidence intervals (CIs) for tertiles of analyte concentration were calculated using conditional logistic regression. results: TGCT cases had elevated concentrations of p,p´-DDE (tertile 3 vs. tertile 1 OR (OR T3 ) 2.2; 95% CI, 0.7-6.5; p Wilcoxon = 0.07), oxychlordane (OR T3 3.2; 95% CI, 0.6-16.8; p Wilcoxon = 0.05), trans-nonachlor (OR T3 2.6; 95% CI, 0.7-8.9; p Wilcoxon = 0.07), and total chlordanes (OR T3 2.0; 95% CI, 0.6-7.2; p Wilcoxon = 0.048) compared with controls, although no ORs were statistically significant. Seminoma cases had significantly lower concentrations of PCB congeners 44, 49, and 52 and significantly higher concentrations of PCBs 99, 138, 153, 167, 183, and 195. cOnclusiOns: Our study provides additional but qualified evidence supporting an association between exposures to p,p´-DDE and chlordane compounds, and possibly some PCB congeners, and TGCT risk.

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“…To identify the chemicals that might interfere with normal androgen activity through l i g a n d -A R i n t e r a c t i o n , t h e U S -E P A h a s recommended the AR reporter gene assay as a screening method in a Tier 1 screening battery (T1S) to detect AR agonists and antagonists [19], and several experimental models [21][22][23][24][25] have been developed. Due to the lack of hAR in HepG2 cells, we transfected the hAR plasmid and reporter gene plasmids to the cells using the transient transfection method.…”

Section: Discussionmentioning

confidence: 99%

The Antiandrogenic Activity of the Fungicide N-(3, 5-Dichlorophenyl) Succinimide in In Vivo and In Vitro Assays

Zhang

Zheng

et al. 2007

Journal of Reproduction and Development

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Abstract. Antiandrogens can cause reproductive disorders in humans and wild animals. In the present study, we tested whether the fungicide N-(3, 5-dichlorophenyl) succinimide (NDPS) acts as an antiandrogen using in vitro and in vivo assays. A transient transfection system based on luciferase activity was utilized for in vitro analysis of the antiandrogeic activity of NDPS. Hershberger assay was used to analyze the antiandrogenic activity of NDPS in rats. The expressions of the androgenresponsive genes testosterone-repressed prostatic message-2 (TRPM-2) and prostate specific binding protein polypeptide C3 (PBP C3) in the rat ventral prostate were measured using real-time PCR. Our results indicated that NDPS can block 5-dehydrotestosterone (DHT)-induced androgen receptor (AR) activity in transiently transfected HepG2 cells (-5 log M). In the Hershberger assay, the weights of the seminal vesicles and levator ani/bulbocavernosus muscles were significantly decreased (P<0.05) in all NDPS groups, and the weights of the ventral prostate, dorsolateral prostate, and Cowper's glands were significantly decreased (P<0.05) in the 100 and 200 mg/kg NDPS groups. NDPS only decreased (P<0.05) the expression of PBP C3 and had no effect on the level of TRPM-2 (P>0.05). In conclusion, NDPS is a moderate antiandrogen that elicits antiandrogenic effects at least partly by antagonizing AR and increasing the expression of PBP C3.

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Androgen receptor activities of p,p′-DDE, fenvalerate and phoxim detected by androgen receptor reporter gene assay

Cited by 55 publications

References 36 publications

The relation between urinary metabolite of pyrethroid insecticides and semen quality in humans

The relation between urinary metabolite of pyrethroid insecticides and semen quality in humans

Pre-Diagnostic Serum Concentrations of Organochlorine Compounds and Risk of Testicular Germ Cell Tumors

The Antiandrogenic Activity of the Fungicide N-(3, 5-Dichlorophenyl) Succinimide in In Vivo and In Vitro Assays

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