Anatomy and Physiology for Biomaterials Research and Development

Ng, Inn Chuan; Pawijit, Pornteera; Tan, Jordon; Yu, Hanry

doi:10.1016/b978-0-12-801238-3.99876-3

Cited by 14 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was reported in the literature that the combination of chitosan and collagen could provide a more compatible environment for cells compared to pure collagen or pure chitosan. 62 In addition, Engler et al observed that the human mesenchymal stem cells (hMSCs) differentiated to different cell types depending on matrix elasticity. 63,64 Our observations are consistent with the literature findings that seeded undifferentiated MSCs showed a tendency to differentiate into round-shape chondroblasts on the composite scaffolds composed of chitosan, collagen, and cellulose that aimed at mimicking cartilage tissue.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of 3D Printed poly(lactic acid) strut and wet-electrospun cellulose nano fiber reinforced chitosan-collagen hydrogel composite scaffolds for meniscus tissue engineering

et al. 2022

View full text Add to dashboard Cite

The main goal of the study was to produce chitosan-collagen hydrogel composite scaffolds consisting of 3D printed poly(lactic acid) (PLA) strut and nanofibrous cellulose for meniscus cartilage tissue engineering. For this purpose, first PLA strut containing microchannels was incorporated into cellulose nanofibers and then they were embedded into chitosan-collagen matrix to obtain micro- and nano-sized topographical features for better cellular activities as well as mechanical properties. All the hydrogel composite scaffolds produced by using three different concentrations of genipin (0.1, 0.3, and 0.5%) had an interconnected microporous structure with a swelling ratio of about 400% and water content values between 77 and 83% which is similar to native cartilage extracellular matrix. The compressive strength of all the hydrogel composite scaffolds was found to be similar (∼32 kPa) and suitable for cartilage tissue engineering applications. Besides, the hydrogel composite scaffold comprising 0.3% (w/v) genipin had the highest tan δ value (0.044) at a frequency of 1 Hz which is around the walking frequency of a person. According to the in vitro analysis, this hydrogel composite scaffold did not show any cytotoxic effect on the rabbit mesenchymal stem cells and enabled cells to attach, proliferate and also migrate through the inner area of the scaffold. In conclusion, the produced hydrogel composite scaffold holds great promise for meniscus tissue engineering.

show abstract

Section: Resultsmentioning

confidence: 99%

Fabrication of 3D Printed poly(lactic acid) strut and wet-electrospun cellulose nano fiber reinforced chitosan-collagen hydrogel composite scaffolds for meniscus tissue engineering

et al. 2022

View full text Add to dashboard Cite

show abstract

“…To assess cell adherence in dependency of substrate crystallinity, cell spreading was moreover investigated by determining the mean cell area on both substrate types. [ 44 ] With a mean spreading area of 700 ± 259 μm on high‐crystalline and 208 ± 45 μm on low‐crystalline microcarriers, L929 mouse fibroblast cells approximately show a 3.4 times larger mean cell area on particles crystallized in concentrated sulfuric acid. This substantial difference in cell/substrate interaction is visualized as histogram in Figure 7D.…”

Section: Resultsmentioning

confidence: 99%

Porous PEDOT:PSS Particles and their Application as Tunable Cell Culture Substrate

Rauer

Bell

Jain

et al. 2021

Adv Materials Technologies

View full text Add to dashboard Cite

Due to its biocompatibility, electrical conductivity, and tissue‐like elasticity, poly(3,4‐ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) constitutes a highly promising material regarding the fabrication of smart cell culture substrates. However, until now, high‐throughput synthesis of pure PEDOT:PSS geometries was restricted to flat sheets and fibers. In this publication, the first microfluidic process for the synthesis of spherical, highly porous, pure PEDOT:PSS particles of adjustable material properties is presented. The particles are synthesized by the generation of PEDOT:PSS emulsion droplets within a 1‐octanol continuous phase and their subsequent coagulation and partial crystallization in an isopropanol (IPA)/sulfuric acid (SA) bath. The process allows to tailor central particle characteristics such as crystallinity, particle diameter, pore size as well as electrochemical and mechanical properties by simply adjusting the IPA:SA ratio during droplet coagulation. To demonstrate the applicability of PEDOT:PSS particles as potential cell culture substrate, cultivations of L929 mouse fibroblast cells and MRC‐5 human fibroblast cells are conducted. Both cell lines present exponential growth on PEDOT:PSS particles and reach confluency with cell viabilities above 95 vol.% on culture day 9. Single cell analysis could moreover reveal that mechanotransduction and cell infiltration can be controlled by the adjustment of particle crystallinity.

show abstract

“…Cellular interaction plays a large role in maintaining cell viability by mediating communication between cells, thus allowing cells to achieve more coordinated functioning, which in turn helps to promote cell integrity, viability, and proliferation. [ 35 ] However, cell–matrix interactions are also a crucial aspect of cell signaling, as they are a pathway by which signals are transferred from external sources to cells. [ 36 ] Enhanced dynamic cell–cell and cell–matrix contacts were highly dependent on the porous structure of the scaffold, and interconnected pores were found to be crucial to transport and interchange gradients for gases, nutrients, signal factors, and growth factors, thereby achieving the best prospects for the proliferation and tissue‐like assembly of the cultured cells.…”

Section: Resultsmentioning

confidence: 99%

Vapor‐Phase Fabrication of Cell‐Accommodated Scaffolds with Multicomponent Functionalization for Neuronal Applications

Christy

et al. 2021

Adv Materials Inter

View full text Add to dashboard Cite

show abstract

Anatomy and Physiology for Biomaterials Research and Development

Cited by 14 publications

References 0 publications

Fabrication of 3D Printed poly(lactic acid) strut and wet-electrospun cellulose nano fiber reinforced chitosan-collagen hydrogel composite scaffolds for meniscus tissue engineering

Fabrication of 3D Printed poly(lactic acid) strut and wet-electrospun cellulose nano fiber reinforced chitosan-collagen hydrogel composite scaffolds for meniscus tissue engineering

Porous PEDOT:PSS Particles and their Application as Tunable Cell Culture Substrate

Vapor‐Phase Fabrication of Cell‐Accommodated Scaffolds with Multicomponent Functionalization for Neuronal Applications

Contact Info

Product

Resources

About