Analyzing the action of evolutionarily conserved modules on HMW-GS 1Ax1 promoter activity

Duan, Luning; Han, Song; Wang, Ke; Pin, Jiang; Gu, Yunsong; Chen, Lin; Mu, Junyi; Ye, Xingguo; Li, Yaxuan; Yan, Yueming; Li, Xiaohui

doi:10.1007/s11103-019-00943-6

Cited by 5 publications

(12 citation statements)

References 62 publications

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“…The accumulation of glutenins and gliadins is mainly controlled at the transcriptional level through a network of transcription factors. The promoter region of HMW-GS, LMW-GS, and α/β-gliadin genes contain several cis-elements, as the GCN4-like motif (GLM) and prolamin box (P-box), which are targeted by basic leucine zipper (bZIP) and DNA binding with one finger (DOF) transcription factors (TFs) [23,[69][70][71][72][73]. Recent studies have provided substantial insights into the presence and function of conserved cis-regulatory modules (CCRM) in the promoters of HMW-GS genes [23,71,74].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Celiac Disease-Related Epitopes and Gluten Fractions, and Identification of Associated Loci in Durum Wheat

et al. 2020

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While durum wheat is a major food source in Mediterranean countries, storage (i.e., gluten) proteins are however responsible for celiac disease (CD), a serious autoimmune disease that occurs in genetically predisposed subjects. Different gluten epitopes—defined as “immunogenic” (IP) and “toxic” (TP) peptides—are involved in the pathology and their content in wheat grain depends on environmental and genetic factors. Detection of IP and TP is not trivial, and no work has been conducted so far to identify the genomic regions associated with their accumulation in wheat. In the present study, a genome-wide association study was performed on a durum wheat collection to identify marker–trait associations (MTAs) between 5730 high quality SNPs and the accumulation of CD-related peptides and gluten protein composition measured in two consecutive cropping seasons (2015/2016 and 2016/2017). High-molecular-weight glutenin subunits (HMW-GS) were more stable between the two years, and differences in total gluten proteins were mainly due to low-molecular-weight glutenin subunits (LMW-GS) and accumulation of gliadins. In the first instance, association tests were conducted on yellow pigment content (YP), a highly inheritable trait with a well-known genetic basis, and several significant MTAs were found corresponding to loci already known for being related to YP. These findings showed that MTAs found for the rest of the measured traits were reliable. In total, 28 significant MTAs were found for gluten composition, while 14 were found to be associated with IP and TP. Noteworthy, neither significant (−log10p > 4.7) nor suggestive (−log10p > 3.3) MTAs for the accumulation of CD-triggering epitopes were found on Gli-A1/Glu-A3 and Gli-B1/Glu-B3 loci, thus suggesting regulatory rather than structural gene effect. A PBF transcription factor on chromosome 5B, known to be involved in the regulation of the expression of CD-related peptides, was identified among the positional candidate genes in the LD-decay range around significant SNPs. Results obtained in the present study provide useful insights and resources for the long-term objective of selecting low-toxic durum wheat varieties while maintaining satisfactory gluten quality.

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Section: Discussionmentioning

confidence: 99%

Characterization of Celiac Disease-Related Epitopes and Gluten Fractions, and Identification of Associated Loci in Durum Wheat

et al. 2020

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“…Two-week-old seedlings, 10-DPA plant tissues, and hand-cut grain sections from various development stages were stained using the β-Galactosidase Reporter Gene Staining Kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing). Semithin sections were prepared following a previously optimized method . Hand-cut grain sections and semithin sections with three biological replicates from each sample were observed on an SV16 fluorescence microscope (Carl Zeiss AG, Jena, Germany) and a multifunctional fluorescence microscope (Zeiss Axio Imager M2, Oberkochen, Germany), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Grains harvested at 15 DPA from transgenic lines were selected to generate fixed sections. Ultrathin section preparation and immunocolloidal gold staining were conducted following a previously described method . Transmission electron microscopy (Hitachi H-7650, Hitachi High-Technologies Corporation, Tokyo, Japan) was used to photograph the slides from three biological replicates.…”

Section: Methodsmentioning

confidence: 99%

Identifying cis-Acting Elements Associated with the High Activity and Endosperm Specificity of the Promoters of Genes Encoding Low-Molecular-Weight Glutenin Subunits in Common Wheat (Triticum aestivum)

Qu,

Wang,

et al. 2023

J. Agric. Food Chem.

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Low-molecular-weight glutenin subunits (LMW-GSs) associated with bread-baking quality and flour nutrient quality accumulate in endosperms of common wheat and related species. However, the mechanism underlying the expression regulation of genes encoding LMW-GSs has not been fully elucidated. In this study, we identified LMW-D2 and LMW-D7, which are highly and weakly expressed, respectively, via the analysis of RNA-sequencing data of Chinese Spring wheat and wheat transgenic lines transformed with 5′ deletion promoter fragments and GUS fusion constructs. The 605-bp fragment upstream of the LMW-D2 start codon could drive high levels of GUS expression in the endosperm. The truncated endosperm box located at the −300 site resulted in the loss of LMW-D2 promoter activity, and a single-nucleotide polymorphism on the GCN4 motif was closely related to the expression of LMW-GSs. TCT and TGACG motifs, as well as the others located on the 5′ distal end, might also be involved in the transcription regulation of LMW-GSs. In transgenic lines, fusion proteins of LMW-GS and GUS were deposited into protein bodies. Our findings provide new insights into the mechanism underlying the transcription regulation of LMW-GSs and will contribute to the development of wheat endosperm as a bioreactor for the production of nutraceuticals, antibodies, vaccines, and medicinal proteins.

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Section: Gene Expressionmentioning

confidence: 99%

“…The mechanism that regulates HMW-GS expression remains largely unclear [47]. Some conserved cis-acting elements related to the expression of storage proteins have been identified in HMW-GS gene promoters [47][48][49][50]. To date, storage protein activator (SPA), a member of the basic leucine zipper (bZIP) family, has been identified to play a key role in the regulation of wheat grain storage protein synthesis [51][52][53][54][55].…”

Section: Gene Expressionmentioning

confidence: 99%

High-Molecular-Weight Glutenin Subunits: Genetics, Structures, and Relation to End Use Qualities

Yang

2020

IJMS

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High-molecular-weight glutenin subunits (HMW-GSs) are storage proteins present in the starchy endosperm cells of wheat grain. Encoding the synthesis of HMW-GS, the Glu-1 loci located on the long arms of group 1 chromosomes of the hexaploid wheat (1A, 1B, and 1D) present multiple allelism. In hexaploid wheat cultivars, almost all of them express 3 to 5 HMW-GSs and the 1Ay gene is always silent. Though HMW-GSs are the minor components in gluten, they are crucial for dough properties, and certain HMW-GSs make more positive contributions than others. The HMW-GS acts as a “chain extender” and provides a disulfide-bonded backbone in gluten network. Hydrogen bonds mediated by glutamine side chains are also crucial for stabilizing the gluten structure. In most cases, HMW-GSs with additional or less cysteines are related to the formation of relatively more or less interchain disulfide bonds and HMW-GSs also affect the gluten secondary structures, which in turn impact the end use qualities of dough.

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Analyzing the action of evolutionarily conserved modules on HMW-GS 1Ax1 promoter activity

Cited by 5 publications

References 62 publications

Characterization of Celiac Disease-Related Epitopes and Gluten Fractions, and Identification of Associated Loci in Durum Wheat

Characterization of Celiac Disease-Related Epitopes and Gluten Fractions, and Identification of Associated Loci in Durum Wheat

Identifying cis-Acting Elements Associated with the High Activity and Endosperm Specificity of the Promoters of Genes Encoding Low-Molecular-Weight Glutenin Subunits in Common Wheat (Triticum aestivum)

High-Molecular-Weight Glutenin Subunits: Genetics, Structures, and Relation to End Use Qualities

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