Analyzing life-history traits and lipid storage using CARS microscopy for assessing effects of copper on the fitness of Caenorhabditis elegans

Fueser, Hendrik; Majdi, Nabil; Haegerbaeumer, Arne; Pilger, Christian; Hachmeister, Henning; Greife, Paul; Huser, Thomas; Traunspurger, Walter

doi:10.1016/j.ecoenv.2018.03.037

Cited by 11 publications

(6 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although Cu is essential, in excess or deficiency it has detrimental effects on brood size and life span and causes an increase in generation time and impairment of development (manifested as reduced body size and growth) [4,16,23,34,43,44]. Since Cu affects reproduction and life-span, it consequently influences population growth.…”

Section: Resultsmentioning

confidence: 99%

The effect of metal mixture composition on toxicity to C. elegans at individual and population levels

et al. 2019

View full text Add to dashboard Cite

The toxicity of zinc (Zn), copper (Cu), and cadmium (Cd) to the nematode Caenorhabditis elegans was characterised under single metal and mixture scenarios at different organisational levels. The effects on population size and body length were investigated at two concentrations corresponding to the 24 h LC5 and LC20 levels. Metal toxicity was dependent on metal concentration, exposure time and mixture composition. Populations exposed to LC20 levels of Cd, ZnCu, CuCd and ZnCuCd plummeted, while for all LC5 concentrations, population size continued to increase, albeit that single metals were less harmful than mixtures. Combinations of the LC20 concentration of Cd with a range of Zn concentrations showed concentration dependent mitigating effects on population size and antagonistic effects on mortality. By combining effects at different organisational levels, more insight into metal toxicity was obtained. Metal effects were more evident on population size than on body length or mortality, suggesting that population size could be considered as a sensitive endpoint. Furthermore, our observations of ZnCd mixture effects at the individual and population levels are consistent with literature data on the dose-dependent expression of the cdf -2 gene, which is involved in mediation of Zn and Cd toxicity.

show abstract

Section: Resultsmentioning

confidence: 99%

The effect of metal mixture composition on toxicity to C. elegans at individual and population levels

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The same difficulties were due to the scarcity of studies related to the "locomotion and substrate relation" trait which has been investigated almost exclusively in nematodes, rotifers, some crustaceans and gastrotrichs e.g., [55,56]. Majdi et al [21] suggested using laboratory populations with mesocosms to study certain traits that otherwise would not be directly observable in the field e.g., [57,58].The results obtained also indicate that the traits receiving the least interest are respiration and thermal preference. These traits, on the other hand, require an indepth study, especially considering the future climate change scenarios that will dramatically affect the coastal areas of lakes [59,60].…”

Section: Discussionmentioning

confidence: 99%

An Overview of Studies on Meiofaunal Traits of the Littoral Zone of Lakes

Cifoni

Boggero

Galassi

et al. 2021

Water

View full text Add to dashboard Cite

We carried out an overview of the studies on the traits of the meiofauna of the littoral zone of lakes to investigate the question relating to the Raunkiaeran shortfall (lack of knowledge on biological traits). For this purpose, we selected a series of keywords associated with response and effect traits (feeding habits, locomotion and substrate relation, body size, shape and mass, life history, reproductive strategy, respiration and thermal tolerance) and we counted the relative frequency of occurrence in a set of scientific papers retrieved from Web of Science. The results showed that, except for the traits related to diet and feeding habits, the Raunkiaeran shortfall is very pronounced for all meiofaunal taxa of the littoral zone of lakes, especially for those related to soft-bodied organisms. The reason behind this deficiency concerns many aspects ranging from the high taxonomic expertise required to the intrinsic difficulties of observing organisms of such a small size. The relationship with temperature has not been sufficiently explored and formalized in any of the examined traits; this research aspect needs to be rapidly addressed since the prospects of climate change impacts on lake littorals are expected to be particularly severe.

show abstract

“…In microscopic organisms, coherent anti-Stokes Raman spectroscopy can be used to non-invasively observe lipid droplets in tissues (e.g. Fueser et al, 2018).…”

Section: Body Conditionmentioning

confidence: 99%

There's no harm in having too much: A comprehensive toolbox of methods in trophic ecology

et al. 2018

Self Cite

View full text Add to dashboard Cite

Trophic ecology is the study of feeding interactions and food acquisition by organisms. It includes the causes and consequences of those behaviours at all levels of biological organisation. As a field of research, it crosses many disciplinary boundaries and provides knowledge that is pertinent to many other areas of ecology. Here we list and categorise the methods available to trophic ecologists whose toolbox has broadened considerably in recent years. They encompass empirical and numerical approaches with focus ranging from molecules to ecosystems. We further examine the relationship of each method to features such as the scale of observation (from microbes to largest organisms) and organisational level (from individuals to ecosystems) as well as the ecological question the method is capable of answering (from detecting predator-prey relationships to studying implications and consequences at different scales). Our survey reveals a very wide range of methodologies, each more-or-less appropriate for a particular line of research. It also identifies deficits, for example, trophic interactions at microscopic scales, for which empirical methods have hardly been used, as well as trophic models that have failed to consider fluxes at the ecosystem scale. Furthermore, we note that the combination of methodologies remains under-exploited despite great opportunities to solve complex ecological questions and to foster the emergence of new insights and hypotheses regarding organism, population and/or ecosystem properties.

show abstract

Analyzing life-history traits and lipid storage using CARS microscopy for assessing effects of copper on the fitness of Caenorhabditis elegans

Cited by 11 publications

References 44 publications

The effect of metal mixture composition on toxicity to C. elegans at individual and population levels

The effect of metal mixture composition on toxicity to C. elegans at individual and population levels

An Overview of Studies on Meiofaunal Traits of the Littoral Zone of Lakes

There's no harm in having too much: A comprehensive toolbox of methods in trophic ecology

Contact Info

Product

Resources

About