“…Mitchell, 1993;c Essington, 2004;d Appelo and Postma, 1993 Analytical Procedure: Given the low concentrations (sub-μg/L to low-μg/L) to be detected, the analytical step had to be as sensitive and as selective as possible. Several recent studies have focused on identifying an analytical procedure for the analysis and detection of steroid hormones in various environmental ecosystems (Baronti et al, 2000;Cathum and Sabik, 2001;Lόpez de Alda and Barcelό, 2001;Petrović et al, 2001;Snyder et al, 2003). However, a unique methodology has not yet been defined for such practices; therefore, an analytical procedure was developed for these studies.…”