Analysis on phenotype, catalpol accumulation and methylation of Rehmannia glutinosa

HuiNa, Wang; BingYi, Li; Chen, Feifei; Zhou, Yanqing; Li, Jiaojiao; Xue, Zhenyi; HongYing, Duan

doi:10.30848/pjb2019-2(13)

Cited by 8 publications

(12 citation statements)

References 16 publications

(17 reference statements)

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“…glutinosa belongs to Scrophulariaceae Rehmannia and is rich in sugars, glycosides, alcohols, terpenoids, and other important components; more than 30 kinds of terpenoids have been discovered so far, such as catalpol, motherwort, arachidin, dihydroanthraquinone, glucoside A, D, and so on (Liu et al, 2012). Catalpol is a higher content of terpenoids in R. glutinosa, is one of the criteria evaluating medicinal effects of R. glutinosa (Wang et al, 2019), and has obvious pharmacological effects on osteoporosis, nervous system, the cardiovascular-cerebrovascular system, and the immune system, as well as having the effect of lowering blood glucose and regulating blood lipid (Huang et al, 2010;Shieh et al, 2011), Abbreviations: BCA, bicinchonininc acid; BP, biological process; CAC2, Biotin carboxylase; CC, cell component; CID, collision induced dissociation; CTAB, cetyltriethyl ammonium bromide; DMAPP, dimethylallyl diphosphate; DEPs, differentially expressed proteins; MEP, 2-methyl erythritose-4-phosphate; E stage, elongation stage; FASP, filter aided sample preparation; FPP, farnesyl pyrophosphate; FPPS, farnesyl pyrophosphate synthase; I stage, expansion stage; GGPP, geranylgeranyl pyrophosphate; GGPPS, geranylgeranyl pyrophosphate synthase; GO, Gene ontology; GPP, geranyl pyrophosphate; GPPS, geranyl pyrophosphate synthase; ID, identification number; IPP, isopentenyl pyrophosphate; iTRAQ, isobaric tags for relative and absolute quantitation; KEGG, kyoto encyclopedia of genes and genomes; M stage, maturation stage; MF, molecular function; MS, mass spectrometry; MVA, mevalonate; MW, molecular weight; ORF, open reading frame; PGI1, Glucose-6-phosphate isomerase 1; PI, isoelectric point; PPI, protein-protein interaction; qRT-PCR, quantitative real-time PCR; R. glutinosa, Rehmannia glutinosa; TCA cycle, citrate cycle; UTR, untranslated regions.…”

Section: Introductionmentioning

confidence: 99%

Study of Terpenoid Synthesis and Prenyltransferase in Roots of Rehmannia glutinosa Based on iTRAQ Quantitative Proteomics

Chen

Wei

et al. 2021

Front. Plant Sci.

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Rehmannia glutinosa has important medicinal value; terpenoid is one of the main active components in R. glutinosa. In this study, iTRAQ technique was used to analyze the relative abundance of proteins in roots of R. glutinosa, and 6,752 reliable proteins were quantified. GO enrichment results indicated that most proteins were involved in metabolic process or cellular process, 57.63% proteins had catalytic activity, and 65.80% proteins were enriched in membrane-bounded organelle. In roots of R. glutinosa, there were 38 KEGG enrichments with significance, more DEPs were found in some pathways, especially the proteasome pathway and TCA cycle with 15.0% DEPs between elongation stage and expansion stage of roots. Furthermore, five KEGG pathways of terpenoid synthesis were found. Most prenyltransferases belong to FPP/GGPP synthase family, involved in terpenoid backbone biosynthesis, and all interacted with biotin carboxylase CAC2. Compared with that at the elongation stage, many prenyltransferases exhibited higher expression at the expansion stage or maturation stage of roots. In addition, eight FPP/GGPP synthase encoding genes were cloned from R. glutinosa, namely FPPS, FPPS1, GGPS, GGPS3, GGPS4, GGPS5, GPPS and GPPS2, introns were also found in FPPS, FPPS1, GGPS5 and GGPS2, and FPP/GPP synthases were more conservative in organisms, especially in viridiplantae, in which the co-occurrence of GPPS or GPPS2 was significantly higher in plants. Further analysis found that FPP/GGPP synthases of R. glutinosa were divided into three kinds, GGPS, GPPS and FPPS, and their gene expression was significantly diverse in different varieties, growth periods, or tissues of R. glutinosa. Compared with that of GGPS, the expression of GPPS and FPPS was much higher in R. glutinosa, especially at the expansion stage and maturation stage. Thus, the synthesis of terpenoids in roots of R. glutinosa is intricately regulated and needs to be further studied.

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Section: Introductionmentioning

confidence: 99%

Study of Terpenoid Synthesis and Prenyltransferase in Roots of Rehmannia glutinosa Based on iTRAQ Quantitative Proteomics

Chen

Wei

et al. 2021

Front. Plant Sci.

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“…Genomic DNA is extracted from roots or leaves of wheat seedlings by the cetyltriethyl ammonium bromide (CTAB) method [ 40 ]. The yield and purity of genomic DNA are determined at 260 nm with micro-spectrophotometry, and the integrity of genomic DNA is detected by 0.8% agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Response of Wheat DREB Transcription Factor to Osmotic Stress Based on DNA Methylation

Zhu

Yuan

Song

et al. 2021

IJMS

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Dehydration-responsive element-binding protein (DREB) plays an important role in response to osmotic stress. In this study, DREB2, DREB6 and Wdreb2 are isolated from wheat AK58, yet they belong to different types of DREB transcription factors. Under osmotic stress, the transcript expression of DREB2, DREB6 and Wdreb2 has tissue specificity and is generally higher in leaves, but their expression trends are different along with the increase of osmotic stress. Furthermore, some elements related to stresses are found in their promoters, promoters of DREB2 and Wdreb2 are slightly methylated, but DREB6’s promoter is moderately methylated. Compared with the control, the level of promoter methylation in Wdreb2 is significantly lower under osmotic stress and is also lower at CG site in DREB2, yet is significantly higher at CHG and CHH sites in DREB2, which is also found at a CHG site in DREB6. The status of promoter methylation in DREB2, DREB6 and Wdreb2 also undergoes significant changes under osmotic stress; further analysis showed that promoter methylation of Wdreb2 is negatively correlated with their expression. Therefore, the results of this research suggest the different functions of DREB2, DREB6 and Wdreb2 in response to osmotic stress and demonstrate the effects of promoter methylation on the expression regulation of Wdreb2.

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“…Genomic DNA was extracted from roots or leaves of wheat seedlings by cetyltriethyl ammonium bromide (CTAB) method [40]. The yield and purity of genomic DNA were determined at 260 nm with microspectrophotometry, the integrity of genomic DNA was detected by 0.8% agarose gel electrophoresis.…”

Section: Cultivation and Treatment Of Wheat Seedlingsmentioning

confidence: 99%

Response of wheat DREB transcription factor to drought stress based on DNA methylation

Zhu

Jia

Wei

et al. 2021

Preprint

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Background: The growth and development of wheat are seriously influenced by drought, dehydration responsive element binding protein (DREB) plays an important role in the response of plant to drought stress, but epigenetic regulation for gene expression of DREB transcription factor is less studied, especially the regulatory role of DNA methylation has not been reported.Results: In this research, DREB2, DREB6 and Wdreb2 were cloned from wheat AK58, and one 712-bp intron was identified in DREB6. Although AP2/EREBP domains of DREB2, DREB6 and Wdreb2 showed 73.25% identity, they belong to different types of DREB transcription factor. Under drought stress, different transcript expression patterns of DREB2, DREB6 and Wdreb2 were observed, and their expression had tissue specificity, was obviously higher in leaves. Promoters of DREB2, DREB6 and Wdreb2 were further studied, some elements related to stresses were found, and the promoters of DREB2 and Wdreb2 were slightly methylated, but DREB6 promoter was moderately methylated. Compared with the control, the level of promoter methylation in DREB2 and DREB6 decreased after 2 h stress treatment, and then increased, which was opposite in Wdreb2 promoter, the status of promoter methylation in DREB2, DREB6 and Wdreb2 also had significant changes under drought stress. Further analysis showed that promoter methylation of DREB6 and Wdreb2 was negatively correlated with their expression, especially in Wdreb2. Conclusions: Our data suggest the different functions of DREB2, DREB6 and Wdreb2 in response to drought stress, and demonstrate the strong effects of promoter methylation on the regulation of Wdreb2 and DREB6 gene expression.

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Analysis on phenotype, catalpol accumulation and methylation of Rehmannia glutinosa

Cited by 8 publications

References 16 publications

Study of Terpenoid Synthesis and Prenyltransferase in Roots of Rehmannia glutinosa Based on iTRAQ Quantitative Proteomics

Study of Terpenoid Synthesis and Prenyltransferase in Roots of Rehmannia glutinosa Based on iTRAQ Quantitative Proteomics

Response of Wheat DREB Transcription Factor to Osmotic Stress Based on DNA Methylation

Response of wheat DREB transcription factor to drought stress based on DNA methylation

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