Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment

Hui, Wenkai; Wang, Yi; Chen, Xiaoyang; Zayed, Mohamed Zaky; Wu, Guojiang

doi:10.3390/ijms19020432

Cited by 25 publications

(20 citation statements)

References 62 publications

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“…In another study conducted on 4-year-old plants in China, the exogenous application of GA 3 inhibited floral initiation and subsequent flowering [ 58 ]. However, in contrast to these findings, similar studies performed in China have indicated that flowering characteristics such as total flower number, female-to-male flower ratio and floral bud differentiation were improved upon GA 3 treatment on the inflorescence [ 59 , 60 ].…”

Section: Resultsmentioning

confidence: 94%

An integration of phenotypic and transcriptomic data analysis reveals yield-related hub genes in Jatropha curcas inflorescence

et al. 2018

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Jatropha curcas is an oil-rich seed crop with huge potentials for bioenergy production. The inflorescence carries a number of processes that are likely to affect the overall yield potentials; floral development, male-to-female flower ratio, floral abscission and fruit set. In this study, a weighted gene co-expression network analysis which integrates the transcriptome, physical and simple sugar data of J. curcas inflorescence was performed and nine modules were identified by means of hierarchical clustering. Among them, four modules (green4, antiquewhite2, brown2 and lightskyblue4) showed significant correlation to yield factors at p≤0.01. The four modules are categorized into two clusters; cluster 1 of green4 and antiquewhite2 modules correspond to number of flowers/inflorescence, total seed weight/plant, number of seeds/plant, and number of fruits/plant, whereas cluster 2 of brown2 and lightskyblue4 modules correspond to glucose and fructose. Descriptive characterizations of cluster 1 show putative involvement in gibberellin signaling and responses, whereas cluster 2 may have been involved in sugar signaling, signal transductions and regulation of flowerings. Our findings present a list of hub genes for J. curcas yield improvement and reproductive biology enhancement strategies.

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Section: Resultsmentioning

confidence: 94%

An integration of phenotypic and transcriptomic data analysis reveals yield-related hub genes in Jatropha curcas inflorescence

et al. 2018

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“…Taken together, we suggest that a high level of BR could improve the fruit yield, whereas the upregulation of GA and JA might decrease the fruit yield in J. curcas. Recently, we reported that exogenous GA could inhibit the endogenous GA biosynthesis and signaling pathways to improve the female flowers in J. curcas [33]. However, the DEGs associated with JA signaling transduction pathway were up-regulated following GA 3 application to contribute to the pollen development process.…”

Section: Qrt-pcr Validationmentioning

confidence: 98%

“…However, the DEGs associated with JA signaling transduction pathway were up-regulated following GA 3 application to contribute to the pollen development process. In further study, a strategic approach will be to investigate the concentration of endogenous phytohormone after exogenous GA 3 treatment in J. curcas [33].…”

Section: Qrt-pcr Validationmentioning

confidence: 99%

Differential gene expression analyses related to fruit yield of Jatropha curcas L. using RNA-seq

Hui

Yang

et al. 2018

Biotechnology & Biotechnological Equipment

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Jatropha curcas L. seeds possess high oil content. However, the low fruit yield is a bottleneck for its utilisation in biodiesel production. To date, the genes related to fruit yield of J. curcas are still poorly studied. Therefore, this study was conducted to compare the transcriptome profiles of two J. curcas clones with high and low fruit yield. The results showed that 52 381 unigenes were obtained and annotated to 55 orthologous terms. More than 9 653 single nucleotide polymorphisms (SNPs)/Indels and 15 971 SSR loci were identified. In addition, 76 differential expression genes (DEGs) were obtained and mainly down-regulated in a high fruit yield sample. Moreover, the DEGs were significantly enriched in phytohormone signalling pathways, including gibberellin, brassinolide and jasmonic acid. Furthermore, we suggested that some genes might inhibit the initiation of female floral differentiation to decrease the fruit yield in J. curcas. These data will create a reference transcriptome for the genomics database of J. curcas for future studies. We expect that the dataset will serve as a foundation to study the genes function, which help in engineering high-yielding varieties in J. curcas.

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“…Fr1 (Fruit 1) was used as a control sample to estimate the relative expression of the DEGs associated with terpenoid compounds and fatty acid metabolism during the fruit development and maturation process in this study. GraphPad Prism 5 was used to draw the figures [64].…”

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism

et al. 2020

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De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism Abstract Background: Zanthoxylum armatum (Z. armatum) is a highly economically important tree that presents a special numbing taste. However, the underlying regulatory mechanism of the numbing taste remains poorly understood. Thus, the elucidation of the key genes associated with numbing taste biosynthesis pathways is critical for providing genetic information on Z. armatumand the breeding of high-quality germplasms of this species.Results: Here, de novo transcriptome assembly was performed for the five major organs of Z. armatum, including the roots, stems, leaf buds, mature leaves and fruits. A total of 111,318 unigenes were generated with an average length of 1014 bp. Additionally, a large number of SSRs were obtained to improve our understanding of the phylogeny and genetics of Z. armatum. The organ-specific unigenes of the five major samples were screened and annotated via GO and KEGG enrichment analysis. A total of 53 and 34 unigenes that were exclusively upregulated in fruit samples were identified as candidate unigenes for terpenoid biosynthesis or fatty acid biosynthesis, elongation and degradation pathways, respectively. Moreover, 40 days after fertilization (Fr4 stage) could be an important period for the accumulation of terpenoid compounds during the fruit development and maturation of Z. armatum. The Fr4 stage could be a key point at which the first few steps of the fatty acid biosynthesis process are promoted, and the catalysis of subsequent reactions could be significantly induced at 62 days after fertilization (Fr6 stage). Conclusions: The present study realized de novo transcriptome assembly for the five major organs of Z. armatum. To the best of our knowledge, this study provides the first comprehensive analysis revealing the genes underlying the special numbing taste of Z. armatum. The assembled transcriptome profiles expand the available genetic information on this species and will contribute to gene functional studies, which will aid in the engineering of high-quality cultivars of Z. armatum.

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Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment

Cited by 25 publications

References 62 publications

An integration of phenotypic and transcriptomic data analysis reveals yield-related hub genes in Jatropha curcas inflorescence

An integration of phenotypic and transcriptomic data analysis reveals yield-related hub genes in Jatropha curcas inflorescence

Differential gene expression analyses related to fruit yield of Jatropha curcas L. using RNA-seq

De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism

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