Analysis of Tissue Uniformity in Transgenic Apple Plants

Flachowsky, Henryk; Riedel, Marko; Reim, Stefanie; Hanke, Magda‐Viola

doi:10.17660/actahortic.2007.738.33

Cited by 4 publications

(2 citation statements)

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“…On the other hand, few markerless transformants appeared to be chimeric in apple (Malnoy et al , 2010). Regeneration of chimeras following selection has also been reported in other fruit trees (Domínguez et al , 2004; Flachowsky et al , 2007; Faize et al , 2010). Therefore, the production of chimeras is not a specific effect of nonselective conditions.…”

Section: Discussionmentioning

confidence: 52%

“…This approach, as has been reported in apple (Malnoy et al , 2010), requires no gene excision system but does require a high rate of regeneration. Our study is unique for stone fruit species and for demonstrating the results of chimera analyses after 3 years of growth in the greenhouse versus analyses of in vitro shoots (Domínguez et al , 2004; Flachowsky et al , 2007; Faize et al , 2010; Malnoy et al , 2010). The system was used to transfer a potentially agronomically important trait (PPV resistance) under the control of a native Prunus promoter ( Cab ) into the plum genome.…”

Section: Discussionmentioning

confidence: 99%

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A high-throughput transformation system allows the regeneration of marker-free plum plants (Prunus domestica)

Petri

Hily

Vann

et al. 2011

Annals of Applied Biology

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A high-throughput transformation system previously developed in our laboratory was used for the regeneration of transgenic plum plants without the use of antibiotic selection. The system was first tested with two experimental constructs, pGA482GGi and pCAMBIAgfp94(35S) that contain selective marker and reporter genes. Transformation was monitored by GUS detection, and estimated transformation efficiencies were 5.7% and 17.7% for pGA482GGi and pCAMBIAgfp94(35S), respectively. Subsequently, an intron-hairpin-RNA (ihpRNA) construct, carrying the Plum Pox Virus coat protein (ppv-cp) gene, without selectable or reporter marker genes was designed. Five transgenic lines were regenerated as confirmed by DNA blot analysis. We believe that this is the first report on the production of marker-free plants transformed with a potential agronomically important trait in a Prunus species.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

A high-throughput transformation system allows the regeneration of marker-free plum plants (Prunus domestica)

Petri

Hily

Vann

et al. 2011

Annals of Applied Biology

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Agrobacterium-mediated transformation of apricot (Prunus armeniaca L.) leaf explants

et al. 2008

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A protocol for Agrobacterium-mediated stable transformation for scored, whole leaf explants of the apricot (Prunus armeniaca) cultivar Helena was developed. Regenerated shoots were selected using a two-step increased concentrations of paromomycin sulphate. Different factors affecting survival of transformed buds, including possible toxicity of green fluorescent protein (GFP) and time of exposure to high cytokine concentration in the regeneration medium, were examined. Transformation efficiency, based on PCR analysis of individual putative transformed shoots from independent lines was 5.6%, when optimal conditions for bud survival were provided. Southern blot analysis on four randomly chosen PCR-positive shoots confirmed the presence of the nptII transgene. This is the first time that stable transformation of an apricot cultivar is reported and constitutes also one of the few reports on the transformation of Prunus cultivars.

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An antibiotic-based selection strategy to regenerate transformed plants from apricot leaves with high efficiency

et al. 2008

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Analysis of Tissue Uniformity in Transgenic Apple Plants

Cited by 4 publications

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A high-throughput transformation system allows the regeneration of marker-free plum plants (Prunus domestica)

A high-throughput transformation system allows the regeneration of marker-free plum plants (Prunus domestica)

Agrobacterium-mediated transformation of apricot (Prunus armeniaca L.) leaf explants

An antibiotic-based selection strategy to regenerate transformed plants from apricot leaves with high efficiency

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