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Cited by 15 publications
(10 citation statements)
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“…SnMIC10 is a secretory protein targeted to the micronemes, which probably plays a role in host cell invasion. Importantly, its expression shows tight developmental regulation (Hoane et al, 2003). Fig.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…SnMIC10 is a secretory protein targeted to the micronemes, which probably plays a role in host cell invasion. Importantly, its expression shows tight developmental regulation (Hoane et al, 2003). Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Cultures were fixed at various times post-infection for 10 minutes using 4% paraformaldehyde in PBS, followed by permeabilization with 0.25% (w/v) Triton X-100 in PBS. The following primary antibodies were used at the indicated dilutions: affinity purified polyclonal rabbit antiserum against T. gondii acyl carrier protein [ACP; 1:400; kindly provided by G. I. McFadden, University of Melbourne (Waller et al, 1998)], monoclonal antibody 12G10 raised against α-tubulin (1:25; kindly provided by J. Frankel, University of Iowa ], monoclonal mouse and polyclonal rabbit anti-centrin [1:400; kindly provided by J. L. Salisbury, Mayo Clinic (Paoletti et al, 1996)], rat polyclonal anti-SnMic10 (Hoane et al, 2003) and rabbit polyclonal anti-IMC3 (Gubbels et al, 2004). All antibodies were diluted in PBS plus 1% BSA and detected using Alexa Fluor 488-or Alexa Fluor 546-conjugated goat anti-mouse, anti-rabbit or anti-rat antibodies (Molecular Probes).…”
Section: Fluorescence Microscopymentioning
confidence: 99%
“…A putative SnMIC10 sequence exhibited approximately 30% identical to the TgMIC10 and NcMIC10 orthologues of T. gondii and N. caninum , respectively, and examination of the native protein in merozoites using antiserum raised against recombinant SnMIC10 revealed characteristics consistent with it being a microneme protein of S. neurona (Hoane et al, 2003). A dithiol-dependent nucleoside triphosphate hydrolase (SnNTP1) was similarly identified based on sequence similarity to the two TgNTPase isoforms of T. gondii (Zhang et al, 2006).…”
Section: In Vitro Cultivation Cell and Molecular Biologymentioning
confidence: 99%
“…Although localization to the apical end of the merozoite was unexpected for SnNTP1, it was found to be part of the secreted fraction of S. neurona , consistent with it being a dense granule protein. Interestingly, both SnMIC10 and SnNTP1 are not expressed for much of S. neurona intracellular development, with these two proteins seen only in late schizonts containing newly-forming daughter merozoites (Hoane et al, 2003; Zhang et al, 2006). While this was expected for SnMIC10 since microneme proteins participate in host cell invasion and should not be needed during endopolygeny, it was unanticipated for SnNTP1 since the TgNTPases are important for intracellular growth of T. gondii (Asai et al, 2002; Nakaar et al, 1999).…”
Section: In Vitro Cultivation Cell and Molecular Biologymentioning
confidence: 99%
“…Briefly, for IFAs of intracellular parasites, infected confluent HFF monolayers were fixed for 20 min in 4% paraformaldehyde in phosphate‐buffered saline (PBS) or with cold methanol for 6 min, permeabilised with 0.2% Triton X‐100, blocked with 10% fetal calf serum in PBS, and then incubated with primary antibodies: anti‐HA (Roche) 1:100, anti‐GFP (Abcam) 1:2,000, anti‐Myc 1:100 (Santa Cruz Biotechnology), anti‐SAG1 1:1,000 (Couvreur, Sadak, Fortier, & Dubremetz, ), anti‐MIC3 1:500 (Achbarou, Mercereau‐Puijalon, Autheman, et al, ), anti‐proMIC3 1:200 (Cerede, Dubremetz, Bout, & Lebrun, ), anti‐ROP1 1:1,000 (Leriche & Dubremetz, ), anti‐ROP7 1:1,000 (kindly provided by Dr Peter Bradley), anti‐AMA1 1:1,000 (Lamarque et al, ), anti‐GRA3 1:500 (Achbarou, Mercereau‐Puijalon, Sadak, et al, ), anti‐CPL 1:500 (Larson et al, ), anti‐proM2AP 1:400 (kindly provided by Dr Vern Carruthers), anti‐proROP4 1:100 (kindly provided by Dr Gary Ward), anti‐MIC2 1:400 (Achbarou, Mercereau‐Puijalon, Autheman, et al, ), anti‐RON2 1:500 (Besteiro, Michelin, Poncet, Dubremetz, & Lebrun, ), anti‐GAP45 1:2,000 (Frenal et al, ), anti‐GAMA 1:500 (Huynh & Carruthers, ), anti‐MIC5 1:500 (Brydges et al, ), anti‐MIC6 1:1,000 (Reiss et al, ), anti‐MIC10 1:500 (Hoane et al, ), anti‐PLP1 1:500 (Kafsack et al, ), anti‐MIC1 1:500 (Reiss et al, ), anti‐MIC4 1:1,000 (Reiss et al, ), anti‐SUB1 1:500 (Miller, Binder, Blackman, Carruthers, & Kim, ), and anti‐ARO 1:1,000 (Mueller et al, ), followed by goat anti‐rabbit or goat anti‐mouse or goat anti‐rat immunoglobulin G conjugated to Alexa Fluor 488 or Alexa Fluor 594 (Molecular Probes, Invitrogen). Coverslips were mounted onto microscope slides using Immumount (Calbiochem).…”
Section: Methodsmentioning
confidence: 99%