Analysis of the recent antipsychotic aripiprazole in human plasma by capillary electrophoresis and high-performance liquid chromatography with diode array detection

Musenga, Alessandro; Saracino, Maria Addolorata; Spinelli, Domenico; Rizzato, Egon; Boncompagni, Giancarlo; Kenndler, Ernst; Raggi, Maria Augusta

doi:10.1016/j.aca.2008.02.046

Cited by 43 publications

(35 citation statements)

References 20 publications

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“…The liquidliquid extraction procedure employed in the present work gave consistent and reproducible recoveries for ARP at all QC levels. The present method has the shortest chromatographic run time compared to all other methods developed for ARP in human plasma [13][14][15][18][19][20][21]. Similarly, the plasma volume required for processing is much lower compared to these methods and identical with one previous report [15].…”

Section: Discussionsupporting

confidence: 51%

“…Previous reports have used either liquid-liquid extraction (LLE) [13,15,18,20,21] or solid phase extraction (SPE) [14,19] for quantitative extraction of ARP from human plasma. However, protein precipitation was tried initially (due to high protein binding of the drug) using methanol, ethanol and acetonitrile as precipitating agents, but the response was insufficient with some ion suppression.…”

Section: Methods Developmentmentioning

confidence: 99%

“…Several analytical methods are reported to quantify ARP in different biological matrices, either alone [11][12][13][14][15][16], together with its active metabolite [17][18][19][20][21], or in combination with other antipsychotic and antidepressant drugs [22][23][24][25]. Therapeutic monitoring of ARP in serum samples by highperformance liquid chromatography (HPLC) with column-switching and spectrophotometric detection has been proposed by Kirschbaum and coworkers [11].…”

Section: Introductionmentioning

confidence: 99%

“…An improved LC-MS/MS method to determine serum levels of ARP and its metabolite in patients has been proposed by Caloro and co-workers [17]. All other methods [13][14][15][18][19][20][21] describe estimation of ARP in human plasma by gas chromatography-mass spectrometry (GC-MS) [19], capillary electrophoresis [14], HPLC [14,20], or LC-MS/MS [13,15,18,21] techniques. The salient features of these methods have been compiled in Table I.…”

Section: Introductionmentioning

confidence: 99%

“…The salient features of these methods have been compiled in Table I. Majority of these methods have a sensitivity which is ≥2 ng mL −1 [13-15, 19, 20] with very long chromatographic run time (5-17 min) [13][14][15][18][19][20][21]. Kubo et al [18] have developed a highly sensitive method for ARP and its active metabolite with a linear calibration range of 0.1-100 ng mL −1 .…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a rapid and sensitive LC-MS/MS method for the determination of aripiprazole in human plasma: Application to a bioequivalence study

Patel¹,

Sharma²,

Patel³

et al. 2014

Acta Chromatographica

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Summary.A rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay has been proposed for the determination of aripiprazole in human plasma. The analyte and propranolol as internal standard (IS) were extracted from 200 µL of human plasma via liquid-liquid extraction using methyl tert-butyl ether under alkaline conditions. The best chromatographic separation was achieved on an Aquasil C18 (100 × 2.1 mm, 5 µm) column using methanol-deionized water containing 2 mM ammonium trifluoroacetate and 0.02% formic acid (65:35, v/v) as the mobile phase under isocratic conditions. Detection of analyte and IS was done by tandem mass spectrometry, operating in positive ion and multiple reaction monitoring (MRM) acquisition mode. The method was fully validated for its selectivity, interference check, sensitivity, carryover check, linearity, precision and accuracy, reinjection reproducibility, recovery, matrix effect, ion suppression/enhancement, stability, ruggedness, and dilution integrity. The assay was linear over the concentration range of 0.10-100 ng mL −1 for aripiprazole. The intra-batch and inter-batch precision (%CV) was ≤4.8%, while the mean extraction recovery was >96% for aripiprazole across quality control levels. The method was successfully applied to a bioequivalence study of 10 mg aripiprazole orally disintegrating tablet formulation in 27 healthy Indian subjects under fasting and fed condition. The reproducibility in the measurement of study data was demonstrated by reanalysis of 260 incurred samples.

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Section: Discussionsupporting

confidence: 51%

Section: Methods Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development and validation of a rapid and sensitive LC-MS/MS method for the determination of aripiprazole in human plasma: Application to a bioequivalence study

Patel¹,

Sharma²,

Patel³

et al. 2014

Acta Chromatographica

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Abacavir

2010

Analytical Methods for Therapeutic Drug Monitoring and Toxicology

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Determination of aripiprazole in rat plasma and brain using ultra‐performance liquid chromatography/electrospray ionization tandem mass spectrometry

Feng

Terry

Bartlett

2012

Biomedical Chromatography

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Aripiprazole is an important antipsychotic drug. A simple, sensitive and rapid ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed and validated for the simultaneous quantification of this compound in rat plasma and brain homogenate. The analyte was extracted from rat plasma and brain homogenate using a weak cation exchange mixed-mode resin-based solid phase extraction. The compound was separated on an Agilent Eclipse Plus C(18) (2.1 × 50 mm, 1.8 µm) column using a mobile phase of (A) 0.1% formic acid aqueous and (B) acetonitrile with gradient elution. The analyte was detected in positive ion mode using multiple reaction monitoring. The method was validated and the specificity, linearity, limit of quantitation (LOQ), precision, accuracy, recoveries and stability were determined. The LOQ was 0.5 ng/mL for aripiprazole in plasma and 1.5 ng/g in brain tissue. The MS response was linear over the concentration range 0.5-100 ng/mL for aripiprazole in plasma and 1.5-300 ng/g in brain tissue. The precision and accuracy for intra-day and inter-day were better than 14%. The relative and absolute recoveries were above 72% and the matrix effects were low. This validated method was successfully used to quantify the rat plasma and brain tissue concentrations of the analyte following chronic treatment with aripiprazole.

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Analysis of the recent antipsychotic aripiprazole in human plasma by capillary electrophoresis and high-performance liquid chromatography with diode array detection

Cited by 43 publications

References 20 publications

Development and validation of a rapid and sensitive LC-MS/MS method for the determination of aripiprazole in human plasma: Application to a bioequivalence study

Development and validation of a rapid and sensitive LC-MS/MS method for the determination of aripiprazole in human plasma: Application to a bioequivalence study

Abacavir

Determination of aripiprazole in rat plasma and brain using ultra‐performance liquid chromatography/electrospray ionization tandem mass spectrometry

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