Analysis of the murine sperm surface with monoclonal antibodies

Crichton, David; Cohen, Brian

doi:10.1530/jrf.0.0680497

Cited by 12 publications

(5 citation statements)

References 23 publications

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“…The 16000-Mr antigen was synthesized mainly in the caput epididymis, whereas the other three antigens appeared to be produced exclusively in the cauda epididymis. Crichton & Cohen (1983) described the production of a monoclonal antibody against a 69000-Mr antigen which was located on the acrosomal cap of mouse spermatozoa, and this is possibly the same antigen as the 66000-Mr species described here. Schmell et al (1982a,b) described a monoclonal antibody that reacted with a 68000-Mr protein on the tail of mouse spermatozoa, and Gaunt (1982) produced an antibody against a 28000-Mr cell-surface autoantigen of spermatogenesis.…”

Section: Discussionmentioning

confidence: 63%

Detection and localization of antigens on the surface of mouse spermatozoa. Antigen synthesis in the epididymis

Murphy¹,

Carroll²

1987

Biochemical Journal

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Spermatozoa achieve functional maturity during their transit through the epididymis and this maturation process is accompanied by changes in the composition and proteins of their surface. The addition of secretory products from the epididymis to the plasma membranes of the spermatozoa is considered to be a prerequisite for the acquisition by the spermatozoa of the capacities for forward motility and ovum recognition. An antibody was purified from an antiserum raised in the rabbit against fluid from the cauda epididymis of the mouse. This antibody, in combination with fluorescein isothiocyanate-conjugated goat anti-rabbit antibody, was used to demonstrate a progressive increase in the synthesis and secretion of antigens along the length of the epididymis. Immunoaffinity chromatography of [35S]methionine-labelled proteins, synthesized by segments of the epididymis maintained in vitro, showed that the predominant protein synthesized by the cauda, but not by the caput, epididymis, migrated on electrophoresis with an apparent Mr of 26,000. This same protein was the major antigen found on the plasma membrane of cauda spermatozoa that had been radioactively labelled with the non-penetrating probe isethionyl [1-14C]acetimidate.

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Section: Discussionmentioning

confidence: 63%

Detection and localization of antigens on the surface of mouse spermatozoa. Antigen synthesis in the epididymis

Murphy¹,

Carroll²

1987

Biochemical Journal

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“…A possible interpretation of these results is that the antigen is modified or partially lost during maturation. A 69K antigen on the anterior acrosome of mouse sperm was also found by radioimmunoassay to be present on testicular cells (Crichton and Cohen, 1983). Only 42% of epididymal sperm were positive by IIF.…”

Section: Monoclonal Antibodies Have Been Used To Study Sperm Surface mentioning

confidence: 78%

“…This latter antigen, referred to as examine the species specificity of antigenic sperm maturation antigen 4 (SMA 41, apdeterminants (Feuchter et al, 1981; Schmell pears on the sperm tail during maturation et al., 1982b;Gaunt, 1982;Primakoff et al, (Feuchter et al, 1981). Other studies on SMA 1983; Crichton and Cohen, 1983;Lee et al, 4 demonstrated that sperm retained in the 1984a,b; Koyama et al, 1984) and to follow ductuli efferentes following ligation did not the fate of sperm components after fertiliza-acquire the antigen, suggesting that its aption (Gaunt, 1983). Other studies have used pearance is not due to changes intrinsic to McAbs to detect autoantigens (Lee et al, the sperm but that the epididymal environ1984a) or to test the putative roles of sperm ment is required (Vernon et al, 1982).…”

Section: Monoclonal Antibodies Have Been Used To Study Sperm Surface mentioning

confidence: 97%

Immunodissection of sperm surface modifications during epididymal maturation

et al. 1985

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Mammalian spermatozoa undergo changes in morphology, composition, and function during transit through the epididymis. These changes correlate with acquisition by sperm of the ability to fertilize ova. It has been found that sperm from the cauda epididymidis, but not those from the caput epididymidis, are able to bind to the zona pellucida. This would imply a modification in sperm surface characteristics. Biochemical and immunological studies have demonstrated changes in sperm surface composition during epididymal maturation. These changes involve addition of epididymal secretory products to the sperm surface, loss or alteration of existing sperm surface molecules, and possibly the unmasking of preexisting molecules or epitopes. Several laboratories have studied the epididymal secretory proteins in the rat, but a consensus has not been reached on the identification, characterization, source, and sperm surface association of these proteins. Monoclonal antibodies are beginning to be used to characterize sperm surface components and sperm maturation antigens. They are proving to be valuable tools for the dissection of epididymal maturation when used in conjunction with biochemical and physiological approaches.

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“…That antigen has a molecular weight of 28,000 and does not correspond with any of the major antigens described in this study. Additional antigens present both on spermatozoa and in the testis of various mammalian species have been characterized, primarily on 1D gels [Teuscher et al, 1982;Crichton and Cohen, 1983;Naz et al, 1984a,b]. Although some of these antigens have molecular weights similar to major antigens detected in this study, other parameters such as PI and stage of appearance have not been determined, thereby limiting effective comparison.…”

Section: Discussionmentioning

confidence: 90%

Immunochemical identification of multiple cell surface antigens appearing during specific stages of mouse spermatogenesis

O’Brien

Millette

1986

Gamete Research

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Cell surface antigens that appear in a defined temporal sequence during mouse spermatogenesis were previously detected serologically, but not identified biochemically, with four heterologous antibodies prepared against purified populations of pachytene spermatocytes (AP), round spermatids (ARS), vas deferens spermatozoa (AVDS), and mixed seminiferous cells (ASC) [Millette and Bellvé, J Cell Biol 74:86–97, 1977]. These antigens have now been identified immunochemically on nitrocellulose blots from SDS polyacrylamide gels. Three antisera (AP, ARS, and ASC) recognize a similar subset of determinants on one‐dimensional immunoblots of germ cells and plasma membranes prepared from a mixed population of late spermatogenic cells. Comparisons of minor bands to reveal differences among these antisera. AVDS exhibits the least complex binding pattern. The results indicate that at least ten surface constituents appear during the pachytene stage of meiosis, coincident with a period of maximal RNA and protein synthesis [Monesi, Exp Cell Res 39:197–224, 1965]. Furthermore, two‐dimensional immunoblot comparisons of plasma membranes isolated from pachytene spermatocytes and round spermatids reveal differences between surface determinants detectable at these two spermatogenic stages. For example, ASC recognizes two newly described proteins that are restricted to pachytene spermatocytes (˜ Mr 57,000, pI 6.45) and to round spermatids (˜ Mr 39,500, pI 4.85), respectively.

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Analysis of the murine sperm surface with monoclonal antibodies

Cited by 12 publications

References 23 publications

Detection and localization of antigens on the surface of mouse spermatozoa. Antigen synthesis in the epididymis

Detection and localization of antigens on the surface of mouse spermatozoa. Antigen synthesis in the epididymis

Immunodissection of sperm surface modifications during epididymal maturation

Immunochemical identification of multiple cell surface antigens appearing during specific stages of mouse spermatogenesis

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