Analysis of the midbrain–hindbrain boundary cell fate using a boundary cell‐specific Cre‐mouse strain

Kala, Kaia; Jukkola, Tomi; Pata, Illar; Partanen, Juha

doi:10.1002/dvg.20344

Cited by 10 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Establishment of a specific boundary cell population in the midbrain-r1 border neuroepithelium is likely to contribute to this developmental compartmentalization (Kala et al, 2008;Trokovic et al, 2005). However, floor-plate cells might circumvent these rules, because, in addition to r1, we observed abundant cells labeled by the hindbrain indicator Gbx2…”

Section: Migration Of R1-derived Vmb Gabanmentioning

confidence: 86%

“…The following antibodies were used in this study: goat anti-GFP (Abcam ab6673, 1:500), anti-Otx2 (R&D Systems BAF1979, 1:200), mouse anti-BrdU (GE Healthcare RPN20AB, 1:400), anti-Gata3 (Santa Cruz SC-268, 1:200), anti-Pou4f1 (Santa Cruz sc-8429, 1:400), anti-Nkx2.2 (1:400, DSHB 74.5A5), anti-Nkx6.1 (1:400, DSHB F55A10) and anti-TH (Millipore MAB318, 1:300); rabbit anti--gal (MP Biomedicals 55976, 1:1000), anti-5-HT (Immunostar 20080, 1:5000), anti-Gata2 (Santa Cruz SC-9008 1:200), anti-GFP (Abcam ab290, 1:600), anti-Lmx1a (a gift from Michael German, University of California at San Francisco, San Francisco, CA, 1:750), anti-RFP (Rockland 600-401-379, 1:500) and anti-TH (Millipore AB152, 1:500). X-gal staining was performed as described previously (Kala et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region

et al. 2012

Self Cite

View full text Add to dashboard Cite

SUMMARYGABAergic neurons in the ventral mesodiencephalic region are highly important for the function of dopaminergic pathways that regulate multiple aspects of behavior. However, development of these neurons is poorly understood. We recently showed that molecular regulation of differentiation of the GABAergic neurons associated with the dopaminergic nuclei in the ventral midbrain (VTA and SNpr) is distinct from the rest of midbrain, but the reason for this difference remained elusive. Here, we have analyzed the developmental origin of the VTA and SNpr GABAergic neurons by genetic fate mapping. We demonstrate that the majority of these GABAergic neurons originate outside the midbrain, from rhombomere 1, and move into the ventral midbrain only as postmitotic neuronal precursors. We further show that Gata2, Gata3 and Tal1 define a subpopulation of GABAergic precursors in ventral rhombomere 1. A failure in GABAergic neuron differentiation in this region correlates with loss of VTA and SNpr GABAergic neurons in Tal1 mutant mice. In contrast to midbrain, GABAergic neurons of the anterior SNpr in the diencephalon are not derived from the rhombomere 1. These results suggest unique migratory pathways for the precursors of important GABAergic neuron subpopulations, and provide the basis for understanding diversity within midbrain GABAergic neurons.KEY WORDS: GABAergic neuron, Ventral tegmental area (VTA), Substantia nigra pars reticulata (SNpr), Dopaminergic neuron, Gata2, Tal1, Mouse

show abstract

Section: Migration Of R1-derived Vmb Gabanmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Immunohistochemistry (IHC) was performed as described (Kala et al, 2008) with the following antibodies: rabbit anti-Arx [from Kunio Kitamura, 1:500 (Kitamura et al, 2002)], mouse anti-BrdU (GE Healthcare RPN20AB, 1:400), rabbit anti-cyclin D1 (LabVision, Neomarkers RM-9104-SO, 1:400), rabbit anti-Gata2 (Santa Cruz sc-9008, 1:250), mouse anti-Gata3 (Santa Cruz sc-268, 1:200), goat anti-GFP (Abcam ab6673, 1:500), rabbit anti-GFP (Abcam ab290, 1:600), guinea pig anti-Heslike (Helt, 1:500; from R. Kageyama, Kyoto University, Japan), mouse antiHuC/D (Molecular Probes A21271, 1:800), mouse anti-Mash1 (Ascl1, BD Biosciences 556604, 1:200), mouse anti-Nkx2-2 [Developmental Studies Hybridoma Bank (DSHB) 74.5A5, 1:250], rabbit anti-NPY (Immunostar 22940, 1:800), rabbit anti-p57…”

Section: Brdu Labellingmentioning

confidence: 99%

Transcriptional regulatory mechanisms underlying the GABAergic neuron fate in different diencephalic prosomeres

et al. 2012

Self Cite

View full text Add to dashboard Cite

SUMMARYDiverse mechanisms regulate development of GABAergic neurons in different regions of the central nervous system. We have addressed the roles of a proneural gene, Ascl1, and a postmitotic selector gene, Gata2, in the differentiation of GABAergic neuron subpopulations in three diencephalic prosomeres: prethalamus (P3), thalamus (P2) and pretectum (P1). Although the different proliferative progenitor populations of GABAergic neurons commonly express Ascl1, they have distinct requirements for it in promotion of cell-cycle exit and GABAergic neuron identity. Subsequently, Gata2 is activated as postmitotic GABAergic precursors are born. In P1, Gata2 regulates the neurotransmitter identity by promoting GABAergic and inhibiting glutamatergic neuron differentiation. Interestingly, Gata2 defines instead the subtype of GABAergic neurons in the rostral thalamus (pTh-R), which is a subpopulation of P2. Without Gata2, the GABAergic precursors born in the pTh-R fail to activate subtype-specific markers, but start to express genes typical of GABAergic precursors in the neighbouring P3 domain. Thus, our results demonstrate diverse mechanisms regulating differentiation of GABAergic neuron subpopulations and suggest a role for Gata2 as a selector gene of both GABAergic neuron neurotransmitter and prosomere subtype identities in the developing diencephalon. Our results demonstrate for the first time that neuronal identities between distinct prosomeres can still be transformed in postmitotic neuronal precursors.

show abstract

“…In addition, probes for chicken Gata3 (Lillevali et al, 2007), Ngn2 (Matter-Sadzinski et al, 2001), Slc17a6 and Gad1 (Cheng et al, 2004) were used. IHC was performed as described (Kala et al, 2008). The following antibodies were used: guinea pig anti-Heslike (Helt, 1:500; gift from R. Kageyama, Institute for Virus Research, Kyoto University, Japan), goat anti-HA probe (Santa Cruz sc-805-G, 1:500) and anti-Olig2 (Neuromics GT15132, 1:200), mouse anti-BrdU (GE Healthcare RPN20AB, 1:400), anti-HuC/D (Molecular Probes A21271, 1:500), anti-Lim1/2 [Lhx1, Developmental Studies Hybridoma Bank (DSHB) 4F2, 1:10], anti-Mash1 (Ascl1, BD Biosciences 556604, 1:200), anti-Nkx2-2 (DSHB 74.5A5, 1:250), anti-Nkx6-1 (DSHB F55A10, 1:500) and anti-Pax6 (DSHB PAX6, 1:100), rabbit anti-caspase 3 active (R&D Systems AF835, 1:500), antiGata2 (Santa Cruz sc-9008, 1:250), anti-5-HT (Immunostar 20080, 1:5000), anti-p57 (NeoMarkers RB-1637-P0, 1:500), anti-phospho-histone H3 (Upstate 06-570, 1:500), anti-Sox2 (Millipore AB5603, 1:500) and anti-TH (Millipore AB152, 1:500).…”

Section: In Situ Mrna Hybridization and Immunohistochemistrymentioning

confidence: 99%

Gata2is a tissue-specific post-mitotic selector gene for midbrain GABAergic neurons

et al. 2009

Self Cite

View full text Add to dashboard Cite

Midbrain GABAergic neurons control several aspects of behavior, but regulation of their development and diversity is poorly understood. Here, we further refine the midbrain regions active in GABAergic neurogenesis and show their correlation with the expression of the transcription factor Gata2. Using tissue-specific inactivation and ectopic expression, we show that Gata2 regulates GABAergic neuron development in the mouse midbrain, but not in rhombomere 1, where it is needed in the serotonergic lineage. Without Gata2, all the precursors in the embryonic midbrain fail to activate GABAergic neuron-specific gene expression and instead switch to a glutamatergic phenotype. Surprisingly, this fate switch is also observed throughout the neonatal midbrain, except for the GABAergic neurons located in the ventral dopaminergic nuclei, suggesting a distinct developmental pathway for these neurons. These studies identify Gata2 as an essential post-mitotic selector gene of the GABAergic neurotransmitter identity and demonstrate developmental heterogeneity of GABAergic neurons in the midbrain.

show abstract

Analysis of the midbrain–hindbrain boundary cell fate using a boundary cell‐specific Cre‐mouse strain

Cited by 10 publications

References 19 publications

Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region

Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region

Transcriptional regulatory mechanisms underlying the GABAergic neuron fate in different diencephalic prosomeres

Gata2is a tissue-specific post-mitotic selector gene for midbrain GABAergic neurons

Contact Info

Product

Resources

About