Analysis of the Interaction Between Bacillus coagulans and Bacillus thuringiensis S-layers and Calcium Ions by XRD, Light Microscopy, and FTIR

Babolmorad, Ghazal; Emtiazi, Giti; Emamzadeh, Rahman

doi:10.1007/s12010-014-0799-0

Cited by 3 publications

(2 citation statements)

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“…The role of slpA in bacterial adhesion to chicken epithelium has been suggested previously. Here, we showed that slpA promoted ESC growth (Fagan and Fairweather 2014;Babolmorad et al 2014;Sleytr et al 2007). When the slpA gene was deleted, ERK1/2 phosphorylation in exposed ESCs was inhibited, whereas slpA overexpression induced ERK1/2 phosphorylation.…”

Section: Discussionmentioning

confidence: 91%

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Lactobacillus slpA promotes ESC growth through the ERK1/2 pathway

Yan

et al. 2017

Cytotechnology

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Bacterial surface layers (S-layers) are cell envelope structures ubiquitously found in gram-negative and gram-positive bacteria, including Lactobacillus. S-layers play a role in the determination and maintenance of cell shape as virulence factors, mediate cell adhesion, and regulate immature dendritic and T cells. In this study, we sought to understand the involvement of MAPK serine/threonine kinases in alterations in Endometrial epithelial cells (ESC) growth induced by Lactobacillus crispatus (L. crispatus) slpA, an S-layer protein. We applied various concentrations of L. crispatus to cultured ESCs and observed growth and changes in the phosphorylation status of ERK1/2, JNK, and p38. Similar experiments were conducted using L. crispatus lacking and overexpressing slpA. We found that ESC growth was altered by slpA primarily via ERK1/2. Our findings suggest that L. crispatus slpA promotes ESC growth mainly through an ERK1/2-dependent pathway.

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Section: Discussionmentioning

confidence: 91%

“…Most bacterial S-layers are composed of a single protein species, the S-layer protein, which greatly varies in size among different bacterial genera (Perras et al 2015;Fagan and Fairweather 2014;Babolmorad et al 2014). S-layers are cell envelope structures ubiquitously found in gram-positive and gram-negative bacteria as well as in Archaea.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus slpA promotes ESC growth through the ERK1/2 pathway

Yan

et al. 2017

Cytotechnology

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Enhanced PC12 Cells Proliferation with Self-Assembled S-Layer Proteins Scaffolds

Babolmorad

Emtiazi

Ghaedi

et al. 2014

Appl Biochem Biotechnol

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Finding 3D biocompatible and biodegradable scaffold is important in tissue engineering which plays a critical role in transplanting methods. Several biomaterials, such as poly-L-lactide, poly(lactic-co-glycolic acid), poly(L-lactic acid)/poly(lactide-co-glycolide), alginate, collagen gel, and so on, have been applied as scaffold to culture cells in 3D environment. The most significant problem of the synthetic materials is lack of biocompatibility and bioactivity. Herein, self assemble S-layer proteins are used as a scaffold for PC12 cells culturing. For this purpose, S-layer protein was extracted from Bacillus coagulans HN68. Then, extracted S-layer was studied by SDS page and AFM. Using MTS test and Immunochemistry staining methods, the effect of self assembled S-layer scaffold on proliferation of PC12 cells was assayed. This study provides that S-layer could be an appropriate scaffold for PC12 cells culturing. Even though poly-L-ornithine is a common scaffold in PC12 cells culturing, the results show that (PLO)/S-layer is more protective.

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