Analysis of the genome of fish lymphocystis disease virus isolated directly from epidermal tumours of pleuronectes

Identification of the gene encoding the major capsid protein of fish lymphocystis disease virus

“…The genome of FLCDV has been characterized by molecular cloning and physical mapping Schnitzler et al, 1987). The structure of the genome of FLCDV was found to be circularly permuted and terminally redundant (Darai et al, 1983, a genomic feature shared with frog virus 3 (FV3), Chilo iridescent virus (CIV) and iridescent virus (IV) type 9. The other genomic property common to FLCDV and FV3 is the high level (22 %) of methylation in cytosine residues (Willis & Granoff, 1980;Darai et al, 1983; The nucleotide sequence data reported here have been submitted to GenBank and assigned the accession number L16683.…”

“…Several enzymatic activities were found to be associated with the purified virions of FLCDV, e.g. nucleoside triphosphate phosphohydrolase, thymidine kinase and protein kinase (Fltigel et al, 1982;Darai et al, 1983). The genetic information located between map coordinates 0.675 and 0"691 of the FLCDV genome have been shown to be able to convert thymidine kinasenegative 3T3 cells by biochemical transformation (Scholz et al, 1988).…”

Characterization of the Genome of Insect Iridescent Virus Type 6 by Physical Mapping

“…Structural analysis and morphogenesis studies have demonstrated many common features among the members of the family Iridoviridae. One of these properties is the circular permutation and terminal redundancy of the viral genome which was found for three members of the family, frog virus 3 (FV3) (Goorha & Murti, 1982), fish lymphocystis disease virus (FLDV) (Darai et al, 1983) and Chilo iridescent virus (CIV) (Delius et al, 1984). CIV was isolated by Fukaya & Nasu (1966) from Chilo suppressalis.…”

DNA viruses associated with diseases of marine and anadromous fish

“…This tubular material appeared to have a periodicity suggesting a helical conformation and may be a nucleocapsid. Analyses of the LDV genome has recently been reported (Darai et al, 1983). The genome is a doublestranded, linear DNA molecule with a molecular weight of 93 ___ 44 × 106.…”