Analysis of the correlation between sperm DNA integrity and conventional semen parameters in infertile men

Aydos, Oya Sena; Yükselten, Yunus; Kaplan, Fuat; Sunguroğlu, Asuman; Aydos, Kaan

doi:10.5152/tud.2015.98475

Cited by 28 publications

(20 citation statements)

References 34 publications

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“…2 B) can damage normal regulation of sperm functions such as sperm capacitation, acrosome reaction, and sperm-oocyte fusion 18,19 . It has been reported that ROS can attack the DNA in sperm nucleus causing base modification, DNA damage, and chromatin cross-linking 20,21 , similarly found in our result (Fig. 4 B).…”

Section: Fig 4: No Dna Damage In Testes Of Control Male Mice (A) Andsupporting

confidence: 91%

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2017

IJPSR

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Arsenic has become a major problem in arsenic contaminated areas today. Its consequences on human health have been increasing with an alarming rate. Apart from toxicological effects caused by arsenic accumulation in the body, it causes several disorders including male infertility. Arsenic has been implicated to expedite the generation of ROS and hence lipid per-oxidation leading to DNA damage, apoptosis, and malfunction of several proteins and enzymes. Reduced number of sperm cells and testosterone level is evident in myriad of literatures. Swiss albino mice were grouped as control and arsenic treated. Weight of testes of mice after arsenic treatment was found to be lower than that of control group with pvalue <0.0001. Similarly, sperm count and testosterone levels were also lower in arsenic administered group as compared to control group with significance < 0.0001. Unlikely, MDA level in arsenic treated mice was higher than control mice (pvalue<0.0001). Moreover, histological sections unravels significant issues related to arsenic toxicity and DNA damage can easily be traced in testes of arsenic treated mice. In conclusion, arsenic, being a major toxic agent, has been observed to be a causative agent of male infertility. In addition to Low sperm count, testosterone level and high level of oxidative stress, degeneration of spermatozoa and disorientation in arrangement of luminal epithelium in histological sections and DNA damage in testicular tissue help us put forth that arsenic accumulation is a potent causative agent for male infertility and it should be taken seriously.

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Section: Fig 4: No Dna Damage In Testes Of Control Male Mice (A) Andsupporting

confidence: 91%

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2017

IJPSR

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“…34 The inverse correlated of Sperm DNA fragmentation with the total motility and normal morphology of spermatozoa, could be the reason of subfertility in the patient groups. [36][37][38] Significantly positive correlation recorded between seminal OS, LPO and DNA fragmentation in our study suggests that the observed LPO and DNA damage may have been due to increased ROS production. The high susceptibility of spermatozoa to OS-induced DNA damage can result not only in poor fertilization rates but also lead to poor embryo quality which can cause birth defects and even childhood diseases.…”

Section: Correlation Between Seminal Redox Status and Sperm 4-hne Dnmentioning

confidence: 84%

Effect of seminal redox status on lipid peroxidation, apoptosis and DNA fragmentation in spermatozoa of infertile Saudi males

Fatima

Alwaznah

Aljuraiban

et al. 2020

SMJ

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To assess the effect of seminal redox status on lipid peroxidation)LPO(, apoptosis and integrity of sperm DNA in infertile males. Methods: In this case-control study, the total antioxidant status)TAS(and reactive oxygen species)ROS(levels were analyzed within the seminal plasma of fertile normozoospermic, n=40 and infertile)asthenozoospermic, n=30; oligoasthenoteratozoospermic, n=30(males. Additionally, the level of 4-hydroxynonenal)4-HNE(, DNA fragmentation, and caspase-3 activity were estimated in the spermatozoa. Original Article Results: Significantly)p<0.001(increased seminal ROS level with decreased TAS scores was observed in the infertile groups compared to normozoospermics. The infertile males showed marked elevated)p<0.001(levels of 4-HNE, DNA fragmentation and caspase-3 activity compared to normozoospermics, which was positively correlated to increased seminal ROS levels and negatively to the TAS score in the studied groups. Seminal ROS level was significantly inverse correlated to the semen parameters. Additionally, a strong negative correlation between DNA fragmentation, LPO, caspase-3activity and seminal parameters were observed. Conclusion: Seminal oxidative stress is a potential risk factor for LPO, DNA damage, and apoptosis in spermatozoa, which can affect semen quality and male fertility. Thus, in addition to conventional seminological parameters, measurement of seminal oxidative stress and sperm DNA integrity may also be employed to investigate the functional integrity of spermatozoa at the molecular level.

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“…It was interesting to observe significantly higher %DFI in male factor infertility, indicating that sperm DNA integrity was compromised in these cases. Earlier studies have also reported significantly higher sperm DNA damage in male factor infertility compared to normal men (Høst et al 2000, Saleh et al 2002, Aydos et al 2015, highlighting the significance of sperm DNA integrity as an important factor in male fertility. Another interesting finding was that the concentration of free thiols, an indicator of less highly condensed chromatin, was significantly higher in the unknown factor infertility group compared to the male factor infertility group.…”

Section: Discussionmentioning

confidence: 82%

Relationship of DNA integrity to HRG C633T SNP and ART outcome in infertile couples

et al. 2017

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The status of sperm DNA fragmentation, protamine deficiency, free thiols and disulphide bonds in colloid-selected samples and its relationship to ART outcome or C633T SNP is not known. The objective of this study was to determine these relationships in spermatozoa from men with male factor or unknown factor infertility ( = 118) undergoing fertilisation (IVF) or intracytoplasmic sperm injection (ICSI). Sperm DNA integrity was analysed by flow cytometry using three fluorescent probes (acridine orange, monobromobimane and chromomycin A3). Principal component analysis (PCA) was used to identify the parameters that most influenced fertility. The relationships of sperm DNA integrity with seminal parameters, C633T SNP and ART outcome were established using ANOVA and -test. Sperm concentration and yield after preparation accounted for 27% of the total variance; sperm DNA integrity (%DFI and disulphide bonds) accounted for 16% of the variance in men from infertile couples. Sperm %DFI was significantly higher ( < 0.05) in older men than in younger men. A significant difference ( < 0.01) was observed in %DFI between smokers and non-smokers. Sperm %DFI was significantly higher ( < 0.01) in male factor infertility compared to either female factor or unknown factor infertility while free thiols were significantly higher ( < 0.01) in unknown infertility factor. No significant difference was observed between IVF success/failure in any of the seminal parameters studied. There was a tendency for protamine deficiency to be higher and disulphide concentration to be lower in men with 633T. Such assessments may provide additional useful information about the prognosis for ART outcome, although more research is needed before clinical guidelines can be provided.

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Analysis of the correlation between sperm DNA integrity and conventional semen parameters in infertile men

Cited by 28 publications

References 34 publications

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Effect of seminal redox status on lipid peroxidation, apoptosis and DNA fragmentation in spermatozoa of infertile Saudi males

Relationship of DNA integrity to HRG C633T SNP and ART outcome in infertile couples

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