Analysis of the cannabinoid content of strains available in the New Jersey Medicinal Marijuana Program

Coogan, Thomas A.

doi:10.1186/s42238-019-0011-z

Cited by 6 publications

(7 citation statements)

References 12 publications

(13 reference statements)

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“…Aiming at increasing bioactive substances with medicinal properties in Cannabis, agricultural geneticists and breeders have detected and selected several cannabis varieties or strains that produce high amounts of cannabinoids such as cannabidiol (CBD) [ 13 , 14 , 15 , 16 ], cannabigerol (CBG) [ 15 , 16 , 17 , 18 ], and Δ 9 -tetrahydrocannabinol (Δ 9 -THC) [ 19 , 20 , 21 ]. This phenotypic selection would lead to the formation of varieties rich in specific phytocannabinoids [ 22 ], which should present phytochemical profile stability.…”

Section: Introductionmentioning

confidence: 99%

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

Ioannidis¹,

Tomprou²,

Mitsis³

et al. 2022

Plants

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Simple sequence repeat (SSR) markers were used to evaluate the genetic stability of the acclimatized micropropagated and regenerated plants of a high cannabidiol (H-CBD) and a high cannabigerol (H-CBG) variety of Cannabis sativa L. Shoot regeneration and proliferation were achieved by culturing calli in Murashige and Skoog basal medium (MS) supplemented with several concentrations of 6-benzyladenine (BA) or thidiazuron (TDZ). Calli derived mostly from stem explants, rather than leaves, cultured on MS supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D) or combination of kinetin (KIN) with 1-Naphthaleneacetic acid (NAA) or 2,4-D. Rooting of the regenerated plantlets accomplished on half-strength MS medium supplemented with indole-3-butyric acid (IBA). Previous studies performed have developed an efficient in vitro micropropagation protocol for mass production. Both in vitro methodologies can be employed in genetic breeding via molecular techniques. The genetic stability of micropropagated and regenerated plants was accomplished using twelve SSR primer pairs that produced reproducible and clear bands, ranging from 90 to 330 bp in size, and resulted in amplification of one or two alleles, corresponding to homozygous or heterozygous individuals. The SSR amplification products were monomorphic across all the micropropagated and regenerated plants and comparable to mother plants. The monomorphic banding pattern confirmed the genetic homogeneity of the in vitro cultured acclimatized and mother plants as no somaclonal variation was detected in clones for these specific SSRs. Our results evidently suggest that the developed culture protocols for in vitro multiplication is appropriate and applicable for clonal mass propagation of the C. sativa varieties and demonstrate the reliability of this in vitro propagation system.

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Section: Introductionmentioning

confidence: 99%

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

Ioannidis¹,

Tomprou²,

Mitsis³

et al. 2022

Plants

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show abstract

“…Testing for the cannabinoids, Δ 9 -tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA), and their decarboxylated counterparts, Δ 9 -tetrahydrocannabinol (Δ 9 -THC) and cannabidiol (CBD), are critical in evaluating hemp-based products being brought to market [7]. In the US, hempbased products must remain under certain concentrations of THCA and THC to remain labeled as hemp versus marijuana, a key determination in many areas given the legality of hemp versus marijuana in many municipalities [8].…”

Section: Introductionmentioning

confidence: 99%

“…In the US, hempbased products must remain under certain concentrations of THCA and THC to remain labeled as hemp versus marijuana, a key determination in many areas given the legality of hemp versus marijuana in many municipalities [8]. Additionally, the CBDA and CBD concentrations within products have been related to their analgesic potency, thus accurate measurement and reporting of these values are vital to proper labeling and pricing of these products to protect both the supplier and consumer [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

A proposed method of sample preparation and homogenization of hemp for the molecular analysis of cannabinoids

et al. 2021

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The use of Cannabis sativa, or Hemp, in commercial, recreational, and pharmacological applications is on the rise in the United States and worldwide. Many of these applications have guidelines associated with them dependent on the concentration of cannabinoid molecules that keep the products classified as hemp versus marijuana or that allow the producer to comment on the purity and potency of their product. Herein, we propose a method for homogenization of hemp that results in small particle sizes, uniform samples, and does not alter the cannabinoid concentrations during processing, allowing for optimal and reproducible potency testing. Using a novel “active grinding media” we homogenized commercially available hemp to analyze approximately 100 mg samples of homogenate via sieve analysis and high-performance liquid chromatography to assess the resulting size and potency of the sample when using this methodology. When processing hemp samples with our proposed methodology, we have demonstrated the ability to produce 60.2% of all particles < 1.25 mm with increased cannabinoid recovery compared to homogenates with larger average particle sizes. Maintaining sample temperatures below 35 °C during processing, we showed that our method does not thermally induce decarboxylation reactions that would result in major cannabinoid profile changes. We have developed a method for hemp processing via homogenization that does not alter the cannabinoid profile during processing, while consistently producing small particle sizes in a uniformly processed sample. This method allows for optimal and reproducible hemp processing when evaluating hemp and hemp-based products being brought to commercial markets.

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“…Testing for the cannabinoids, Δ 9 -tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA), and their decarboxylated counterparts, Δ 9tetrahydrocannabinol (Δ 9 -THC) and cannabidiol (CBD), are critical in evaluating hemp-based products being brought to market [7]. In the US, hemp-based products must remain under certain concentrations of THCA and THC to remain labeled as hemp versus marijuana, a key determination in many areas given the legality of hemp versus marijuana in many municipalities [8].…”

Section: Introductionmentioning

confidence: 99%

“…In the US, hemp-based products must remain under certain concentrations of THCA and THC to remain labeled as hemp versus marijuana, a key determination in many areas given the legality of hemp versus marijuana in many municipalities [8]. Additionally, the CBDA and CBD concentrations within products have been related to their analgesic potency, thus accurate measurement and reporting of these values are vital to proper labeling and pricing of these products to protect both the supplier and consumer [6,7,8].…”

Section: Introductionmentioning

confidence: 99%

A Proposed Method of Sample Preparation and Homogenization of Hemp for the Molecular Analysis of Cannabinoids

Morehouse

Ryan

Proctor

et al. 2020

Preprint

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BackgroundThe use of Cannabis sativa, or hemp, in commercial, recreational, and pharmacological applications is on the rise in the United States and worldwide. Many of these applications have guidelines associated with them dependent on the concentration of cannabinoid molecules that keep the products classified as hemp versus marijuana or that allow the producer to comment on the purity and potency of their product. Herein, we propose a method for homogenization of hemp that results in small particle sizes, uniform samples, and does not alter the cannabinoid concentrations during processing, allowing for optimal and reproducible potency testing. MethodsUsing a novel “active grinding media” we homogenized commercially available hemp to analyze approximately 100mg samples of homogenate via sieve analysis and high-performance liquid chromatography to assess the resulting size and potency of the sample when using this methodology. ResultsWhen processing hemp samples with our proposed methodology, we have demonstrated the ability to produce 60.2% of all particles <1.25 mm with increased cannabinoid recovery compared to homogenates with larger average particle sizes. Maintaining sample temperatures below 35° C during processing, we showed that our method does not thermally induce decarboxylation reactions that would result in major cannabinoid profile changes.ConclusionWe have developed a method for hemp processing via homogenization that does not alter the cannabinoid profile during processing, while consistently producing small particle sizes in a uniformly processed sample. This method allows for optimal and reproducible hemp processing when evaluating hemp and hemp-based products being brought to commercial markets.

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Analysis of the cannabinoid content of strains available in the New Jersey Medicinal Marijuana Program

Cited by 6 publications

References 12 publications

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

A proposed method of sample preparation and homogenization of hemp for the molecular analysis of cannabinoids

A Proposed Method of Sample Preparation and Homogenization of Hemp for the Molecular Analysis of Cannabinoids

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