Analysis of steroids in bulk pharmaceuticals by liquid chromatography with light-scattering detection

Asmus, Paul A.; Landis, J. B.

doi:10.1016/s0021-9673(00)96174-0

Cited by 57 publications

(16 citation statements)

References 15 publications

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“…Mitchell et al used an Alltech 3300 ELSD to study twelve compounds analyzed in the HILIC and reversed-phase modes determining the HILIC model typically produced greater sensitivity than the reversed-phase mode [23]. Several reports have shown the increased response for ELSD as the amount on acetonitrile is increased in the mobile phase for mexiletine, triglycerides, hydrocortisone, amikacin and 5-fluorocytosine [15][16][17][18][19].…”

Section: Mobile Phase Characteristicsmentioning

confidence: 99%

Aerosol-based detectors for liquid chromatography

Magnusson

Risley

Koropchak

2015

Journal of Chromatography A

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Section: Mobile Phase Characteristicsmentioning

confidence: 99%

Aerosol-based detectors for liquid chromatography

Magnusson

Risley

Koropchak

2015

Journal of Chromatography A

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“…The theoretical Eq. (1) allows the ELSD to give equivalent responses for related structure substances [18], but also whatever the classes of compounds studied [19]. The comparison of linear regression slopes of tobramycin and colistin sulphate (Table 3) leads to the evaluation of the detection sensitivity for these two active substances.…”

Section: Linearitymentioning

confidence: 99%

Simultaneous quantitation of tobramycin and colistin sulphate by HPLC with evaporative light scattering detection

Clarot

Storme-Paris

Chaminade

et al. 2009

Journal of Pharmaceutical and Biomedical Analysis

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“…For example, evaporative light scattering detectors (ELSD) have been used to detect nonvolatile compounds such as lipids [3], carbohydrates [4], and pharmaceutical compounds [5,6], which exhibit weak optical absorbance in the UV-Visible region. However, ELSD is considered a semi-universal detector because the sensitivity depends strongly on the volatility of the analytes and mobile phases [7].…”

Section: Universal Detection In Hplcmentioning

confidence: 99%

Suspended Microchannel Resonators for Ultralow Volume Universal Detection

et al. 2008

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Universal detectors that maintain high sensitivity as the detection volume is reduced to the sub-nanoliter scale can enhance the utility of miniaturized total analysis systems (ji-TAS). Here the unique scaling properties of the suspended microchannel resonator (SMR) are exploited to show universal detection in a 10 pL analysis volume with a density detection limit of -1 [gg/cm 3 (10 Hz bandwidth) and a linear dynamic range of six decades. Analytes with low UV extinction coefficients such as polyethylene glycol (PEG) 8 KDa, glucose, and glycine are measured with molar detection limits of 0.66 RM, 13.5 gM, and 31.6 JIM, respectively. To demonstrate the potential for real-time monitoring, gel filtration chromatography was used to separate different molecular weights of PEG as the SMR acquired a chromatogram by measuring the eluate density. This work suggests that the SMR could offer a simple and sensitive universal detector for various separation systems from liquid chromatography to capillary electrophoresis. Moreover, since the SMR is itself a microfluidic channel, it can be directly integrated into g-TAS without compromising overall performance. AcknowledgementsFirst of all, I wish to thank Professor Scott Manalis for the opportunity to do my thesis research in his laboratory. Scott has given me invaluable guidance that fosters creativity, teamwork, enthusiasm, and handson research. I also want to thank all group members who contributed to this work: Thomas Burg originally developed the SMR and related setups which I used for my research inline with HPLC system. He also gave me countless advices regarding experiment. Will Grover was an indispensable resource for everything from the knowledge involving HPLC to experimental details. Michel Godin was a good consultant who gave me an insight to research in general. Scott Knudsen helped me dealing with problems involving chemistry and biology with great passion. Yao-Chung (Wesley) Weng gave me a great help in dealing with electronics such as oscillating circuits. Furthermore, the passion of Rumi Chunara, diligence of Andrea Bryan, optimism of Marcio von Muhlen, and discretion of Phillp Dextras has been constant sources of motivation for me. I also thank Ken Babcock and Alison Skelley for valuable discussions.

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Analysis of steroids in bulk pharmaceuticals by liquid chromatography with light-scattering detection

Cited by 57 publications

References 15 publications

Aerosol-based detectors for liquid chromatography

Aerosol-based detectors for liquid chromatography

Simultaneous quantitation of tobramycin and colistin sulphate by HPLC with evaporative light scattering detection

Suspended Microchannel Resonators for Ultralow Volume Universal Detection

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