Analysis of spermidine and spermine in rat liver. Effect of hypophysectomy on polyamine concentrations

Hall, Douglas E.; Symonds, Gabriel; Symonds, David; Brosnan, Margaret E.

doi:10.1139/o78-045

Cited by 9 publications

(6 citation statements)

References 3 publications

(3 reference statements)

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“…For analysis of polyamine concentrations, a portion of homogenate was deproteinized by addition of solid sulphosalicylic acid (40mg/ml of homogenate), protein was removed by centrifugation (20000g for 10min at 4°C), and the clear supernatant was applied to a Beckman amino acid analyser, as described previously (Hall et al, 1978).…”

Section: Assay Of Enzymesmentioning

confidence: 99%

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Polyamine metabolism in liver of young rats

Brosnan¹,

Symonds²,

Hall³

et al. 1978

Biochemical Journal

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Rat liver undergoes a phase of rapid growth during weaning. We followed the changes in polyamine metabolism occurring during this period of natural growth, and compared them with changes in DNA and RNA accumulation. There was a 2.5-fold increase in the number of cells per liver between suckling (18--19 days old) and weaning (30--32 days old) rats. Ornithine decarboxylase activity increased from the low value in 18-day-old rat pups and remained significantly higher (approx. 5--10-fold) than that in adult rats from day 21 to day 34. Putrescine-dependent S-adenosylmethionine decarboxylase activity was slightly but significantly increased during most of this period. Spermidine and RNA concentrations fluctuated in concert, whereas spermine content per cell doubled during the period from day 23 to day 30.

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Section: Assay Of Enzymesmentioning

confidence: 99%

“…3. Putrescine concentration and ratio of activity of ornithine decarboxylase to S-adenosylmethionine decarboxylase in rat liver during and after weaning Putrescine (a) was measured on a Beckman model 121 amino acid analyser (Hall et al, 1978). Each point is the mean ± S.E.M.…”

Section: Putrescine-dependent S-adenosylmethionine Decarboxylase Activitymentioning

confidence: 99%

Polyamine metabolism in liver of young rats

Brosnan¹,

Symonds²,

Hall³

et al. 1978

Biochemical Journal

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“…In particular, the reported effects of spermidine include promoting protein synthesis, anti-inflammation, antioxidation, and promoting hair growth. [34][35][36][37] In terms of elevated SAM levels, the significance of OM-X ® administration is expected to be not only activation of detoxification but also promotion of protein synthesis and anti-aging effects of spermidine.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic analysis of SMP30/GNL knockout mice treated with fermented vegetable and fruit extract (OM-X®)

Wakame

Takahata²,

Miyake³

et al. 2020

FFHD

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Background: A dietary supplement developed in Japan, OM-X®, is the result of extended fermentation of dozens of edible vegetables, fruits, seaweeds, mushrooms, and includes 12 strains of Lactic acid bacteria (LAB) and Bifidobacterium. The supplements powerful antioxidant, anti-inflammatory properties, and regulatory effect on metabolizing liver enzymes have been reported.Senescence marker protein-30 (SMP30)/Gluconolactonase (GNL) Knockout (KO) mice, which lack the ability to biosynthesize Vitamin C (VC) in the body, were used in this study. The mice exhibited decreased antioxidant capacity in the blood, decreased liver function, and had poor hair growth due to the VC deficiency. The aim of the study was to examine the effects of oral administration of OM-X® on VC deficiency from the viewpoint of comprehensive analysis of liver metabolism.Methods: SMP30/GNL KO and C57BL/6 (WT) mice were used in this study. The KO mice were divided into the following three groups: VC non-administration (VC minus), VC (0.15 W/V %) administration (VC plus), and no VC plus 0.6% OM-X® administration (OM-X®) groups (n = 6 per group). Mice were kept under experimental conditions until they were euthanized at week 8 (age: 16 weeks old). Body weight and plasma biochemistry levels were measured.. Metabolomic analyses of harvested livers were performed.Results: In the VC minus group, a significant decrease in body weight and significant changes in plasma biochemistry parameters compared with the WT control or VC plus groups was observed. In the OM-X® group, significant suppression or a suppressive trend was observed compared with the VC minus group. Liver metabolomic analysis showed that total adenylate levels decreased in the VC minus group, whereas OM-X® administration significantly elevated total adenylate levels. In addition, the ratios of Glutathione (GSH)/ Glutathione disulfide (GSSG) and S-adenosylmethionine (SAM)/ S-adenosyl-L-homocysteine (SAH) and total glutathione levels were all significantly higher in the OM-X® group than in the VC minus group. The glycerol 3-phosphate/ Dihydroxyacetone phosphate (DHAP) and putrescine/spermidine ratios were elevated in the VC minus group, whereas it was significantly lower in the OM-X® group.Conclusion: The results showed that in SMP30/GNL KO mice, some organ damage may have occurred during VC deficiency, as indicated by weight loss, hepatic injury, and changes in triglyceride-related markers. OM-X® had an effect on energy charge maintenance by elevating total adenylate levels, inducing antioxidant capacity via enhancing glutathione levels, and promoting protein synthesis, including polyamine synthesis. Altogether, the results revealed that OM-X® prevents the adverse biological changes caused by VC deficiency in SMP30/GNL KO mice.Keywords: OM-X®, SMP30/GNL KO mice, Vitamin C, metabolomic analysis.

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“…Polyamines in liver were determined using a modification of the method described by Hall et al (1978). Freeze-clamped liver and brain were ground into powder form in liquid nitrogen using a mortar and pestle.…”

Section: Methodsmentioning

confidence: 99%

Influence of Avian Aflatoxicosis on the Synthesis of Polyamines

et al. 1987

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Effects were examined of inanition, dietary aflatoxin (2.5 mg/kg), and dietary supplements of threonine, lysine, and arginine on the activities of renal arginase and hepatic ornithine decarboxylase and on the accumulation of polyamines in liver and brain of 24 or 26-day-old broiler cockerels. Aflatoxicosis and inanition lowered the activity of renal arginase by 58 and 37%, respectively. Supplemental dietary threonine (.4%) did not suppress the activity of renal arginase, while fortification of diets of controls with lysine (.53%), but not diets containing aflatoxin, elevated the activity of renal arginase. Supplements of dietary lysine and/or arginine did not influence the hepatic content of putrescine but lowered the concentrations of spermidine and spermine. Aflatoxicosis, but not inanition, increased the activity of hepatic ornithine decarboxylase (ODC; 22-fold), increased hepatic concentrations of putrescine and spermidine, but decreased spermine concentrations. The elevation of hepatic ODC, putrescine, and the ratio of spermidine to spermine parallels the enlargement of the liver caused by aflatoxicosis. Cadaverine and putrescine were not detected in avian brain, while cerebral concentrations of spermidine and spermine were not altered by aflatoxin, inanition, or by supplements of dietary lysine, arginine, or both lysine and arginine.

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Analysis of spermidine and spermine in rat liver. Effect of hypophysectomy on polyamine concentrations

Cited by 9 publications

References 3 publications

Polyamine metabolism in liver of young rats

Polyamine metabolism in liver of young rats

Metabolomic analysis of SMP30/GNL knockout mice treated with fermented vegetable and fruit extract (OM-X®)

Influence of Avian Aflatoxicosis on the Synthesis of Polyamines

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