2020
DOI: 10.1101/2020.04.15.997254
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Abstract: 21Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses an acute threat to public health 22 and the world economy, especially because no approved specific drugs or vaccines are available. 23Pharmacological modulation of metabolism-dependent cellular pathways such as autophagy reduced 24 propagation of highly pathogenic Middle East respiratory syndrome (MERS)-CoV. 25Here we show that SARS-CoV-2 infection limits autophagy by interfering with multiple metabolic 26 pathways and that compound-driven in… Show more

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Cited by 109 publications
(153 citation statements)
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“…60 Interestingly, the anti-helminthic and FDA-approved drug niclosamide has recently been proposed as a potential anti-SARS-CoV-2 agent, 61,62 possibly through its autophagic induction mechanism. 63 It has yet to be elucidated whether panduratin A suppresses SARS-CoV-2 infection via the induction of autophagy, and which pathway is a direct target for this compound.…”
Section: Discussionmentioning
confidence: 99%
“…60 Interestingly, the anti-helminthic and FDA-approved drug niclosamide has recently been proposed as a potential anti-SARS-CoV-2 agent, 61,62 possibly through its autophagic induction mechanism. 63 It has yet to be elucidated whether panduratin A suppresses SARS-CoV-2 infection via the induction of autophagy, and which pathway is a direct target for this compound.…”
Section: Discussionmentioning
confidence: 99%
“…Since SAM is required for the capping of the viral (as well as cellular) RNA (Viswanathan et al, 2020), this might further limit the production of infectious RNA. Indeed, the characterization of cellular metabolomics in response to SARS-CoV-2 infection, and the observed reduction in spermidine, at least suggested that SAM levels were 451 reduced in infected cells (Gassen et al, 2020). However, based on the fact that the purine nucleoside inosine was capable of rescuing virus replication (Fig.…”
Section: Discussion 419mentioning
confidence: 99%
“…The RNA pellet was washed with ethanol, dried and resuspended in nuclease-free water. Quantitative RT-PCR was performed according to a previously established RT-PCR assay involving a TaqMan probe (Corman et al, 2020)…”
Section: Quantitative Rt-pcr For Virus Quantification 163mentioning
confidence: 99%
“…For example, in the context of SARS-CoV-2, endosomes might release viral RNA or particles; they might merge with lysosomes and digest their viral cargo; or they might fuse with autophagosomes (autophagy) and subsequently with lysosomes that digest the cargo 22,52 . SARS-CoV-2 might reprogram cellular metabolism to suppress autophagy and promote viral replication 54 ; conversely, the cell might modify autophagy machinery to decorate viral invaders with ubiquitin for eventual destruction, activate the immune system by displaying parts of the virus, or catabolize excess pro-cytokines. Autophagy might induce cytokine signaling, which could promote protective immune response or engender a destructive storm of cytokines, inflammation and tissue damage 22 .…”
Section: Discussionmentioning
confidence: 99%