Analysis of Pulp Tissue Viability and Cytotoxicity of Pulp Capping Agents

Abstract

The present research study assessed the cell viability and cytotoxic effect of mineral tri-oxide aggregate (MTA), Tetric N-Bond Universal bonding agent, Theracal PT (pulpotomy treatment), and platelet-rich fibrin (PRF) as pulp capping agents on human dental pulp stem cells (hDPSCs). The cells were isolated from the pulp tissue of an extracted healthy permanent third molar. After four passages in Dulbecco’s Modified Eagle’s Medium, the primary cells were employed for the investigation. The test materials and untreated cells (negative control) were subjected to an Methylthiazol-diphenyl-tetrazolium (MTT) cytotoxicity assay and assessed at 24-, 48-, and 72-h intervals. The Wilcoxon matched-paired t-test and Kruskal–Wallis analysis of variance (ANOVA) test were applied (p < 0.05). PRF imparted the highest cell viability at 48 h (p < 0.001), followed by MTA, Theracal PT, and Tetric N-Bond. Similarly, PRF had the highest potential to enhance cell proliferation and differentiation (p < 0.001), followed by Theracal PT, MTA, and the bonding agent at the end of 24 h and 72 h, respectively. Finally, PRF sustained the viability of human primary dental pulp stem cells more effectively than Theracal PT and MTA; however, the application of a Tetric N-Bond as a pulp capping agent was ineffective.

Comparative Analysis of the Physical and Biochemical Properties of Light-cure Resin-modified Pulp Capping Materials

“…7. The results of this study are consistent with those of previous research, indicating that TPT does not exhibit cytotoxicity and supports cell proliferation [61]. Additionally, the results aligned with previous cell toxicity experiments based on the dilution ratios of TPT and TLC [15].…”

Lignin-Decorated SiO2 Nanoparticles for Skin Cleansing Applications

“…Since the material is intended for human skin cleansing applications, cytotoxicity testing is required to guarantee its safety. 30 To calculate sample SiO 2 /lignin#2 for cytotoxicity assessment, the absorbance readings of HaCaT cells, L929 cells, and RAW264.7 cells exposed to samples of different concentrations at 25, 50, 100, 200, and 400% were compared with that of 0% concentration. 31 To obtain the relative percentage of cell viability, as shown in Figures S3−S4, the values of 25, 50, 100, 200, and 400% concentrations remained similar to 0% concentrations for the three cell types, indicating that the cytotoxicity levels of the sample SiO 2 /lignin#2 were relatively low.…”

“…Since the material is intended for human skin cleansing applications, cytotoxicity testing is required to guarantee its safety . To calculate sample SiO 2 /lignin#2 for cytotoxicity assessment, the absorbance readings of HaCaT cells, L929 cells, and RAW264.7 cells exposed to samples of different concentrations at 25, 50, 100, 200, and 400% were compared with that of 0% concentration .…”

Influence of Bioceramic Cements on the Quality of Obturation of the Immature Tooth: An In Vitro Microscopic and Tomographic Study

“…Recently, several studies showed that treatment of the open apex with calcium silicate materials could improve the efficiency of this treatment within one visit [ 7 , 8 , 9 ]. These materials showed their efficiency in different endodontic treatments such as apicoectomy [ 10 ], perforation [ 11 ], pulpotomy [ 12 ], open apex [ 5 ], pulp cupping [ 13 ], and resorption [ 14 ]. These calcium-silicate-based materials, called bioceramics, have excellent physicochemical, mechanical, and biological properties [ 15 ].…”