Analysis of major band of Enterobacter sakazakii by ERIC-PCR and development of a species-specific PCR for detection of Ent. sakazakii in dry food samples

Ye, Yingwang; Wu, Qingping; Zhou, Yanhong; Dong, Xiaohui; Zhang, Jumei

doi:10.1016/j.mimet.2008.07.012

Cited by 15 publications

(9 citation statements)

References 29 publications

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“…The molecular typing methods including Pulsed Field Gel Electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)‐PCR and random amplified polymorphic DNA (RAPD)‐PCR have been used for typing of Cronobacter isolates (Ye et al . ; Ye et al . ; Brengi et al .…”

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, all of C. sakazakii strains were grouped into three clusters, which indicated that there might be subtypes among C. sakazakii strains. The molecular typing methods including Pulsed Field Gel Electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR and random amplified polymorphic DNA (RAPD)-PCR have been used for typing of Cronobacter isolates (Ye et al 2008;Ye et al 2010;Brengi et al 2012), but these methods could not differentiate Cronobacter species. The differentiation of Cronobacter species was reported based on 16S rRNA sequencing; C. sakazakii and C. malonaticus were difficult to differentiate because of their close relationship (Kucerova et al 2010).…”

Section: Resultsmentioning

confidence: 99%

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Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism Typing

Ling

Han

et al. 2015

Journal of Food Safety

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Cronobacter species are foodborne pathogens associated with neonatal meningitis, sepsis and necrotizing enterocolitis through consumption of contaminated powdered milk. However, there are no specific targets used for differentiating Cronobacter species. The DNA-based assay and polyphasic analysis have facilitated the detection and typing of Cronobacter species in foodborne outbreaks. In this study, polymerase chain reaction (PCR) and dot hybridization for detecting Cronobacter spp. was developed targeting gluB gene, then one PCR-restriction fragment length polymorphism (RFLP) typing assay with TaqI digestion was applied for four species differentiation within Cronobacter. Results indicated that the gluB gene is a specific target for detecting Cronobacter and the PCR-RFLP typing assay could conveniently and rapidly differentiate Cronobacter malonaticus, C. dublinensis, C. sakazakii and C. muytjensii. PRACTICAL APPLICATIONSIn this study, polymerase chain reaction (PCR) and dot hybridization targeting gluB for detecting Cronobacter was developed and a PCR-restriction fragment length polymorphism (RFLP) typing assay with TaqI was successfully applied for differentiating four Cronobacter species. Results indicated that gluB is a specific marker for detection of Cronobacter and PCR-RFLP targeting gluB gene product digested with TaqI could conveniently differentiate four Cronobacter species.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism Typing

Ling

Han

et al. 2015

Journal of Food Safety

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“…; Ye et al . ; ). Therefore, to monitor Cronobacter isolates from different sources, a combination of several typing methods should be used to correctly trace the origins of Cronobacter isolates.…”

Section: Resultsmentioning

confidence: 99%

“…The reproductive and stable ERIC‐PCR fingerprinting technique was optimised and applied by Ye et al . (, ). They found that ERIC‐PCR could be used for typing of Enterobacter sakazakii ( Cronobacter sp.)…”

Section: Genotypic Methodsmentioning

confidence: 99%

The Cronobacter sp. in milk and dairy products: Detection and typing

et al. 2014

Int J of Dairy Tech

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Cronobacter is a group of important foodborne pathogens that have been implicated in meningitis, sepsis and necrotising enterocolitis, especially in neonates through consumption of contaminated powdered infant formula (PIF). The detection of PIF contamination is of great concern to the infant formula industry. In this article, phenotypic and genotypic methods for detecting and typing Cronobacter are discussed in relation to minimising and controlling the risk of Cronobacter sp. contamination. To reliably detect and type Cronobacter strains, the use of conventional microbiological methods in combination with molecular assays is recommended.

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“…A number of useful polymorphic techniques, such as RAPD, repetitive-sequence-based PCR (rep-PCR), enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), and amplified fragment length polymorphism (AFLP), have been used for the detection of organism-specific distinctive DNA sequences. [28][29][30][31][32][33][34] Although only a few reports describe specific genetic detection methods applicable at the strain level, Fujimoto et al, 35) Maruo et al, 36) and Tilsala-Timisjärvi & Alatossava 37) reported the use of RAPD for strain-specific detection of Lactococcus lactis ssp. cremoris FC, Lactobacillus casei Shirota, and Lactobacillus rhamnosus Lc 1/3, respectively.…”

Section: )mentioning

confidence: 99%

Development of Strain-Specific PCR Primers for Quantitative Detection of <i>Bacillus mesentericus</i> Strain TO-A in Human Feces

Sato

Seo

Benno³

2014

Biological & Pharmaceutical Bulletin

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Strain-specific polymerase chain reaction (PCR) primers for detection of Bacillus mesentericus strain TO-A (BM TO-A) were developed. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. A 991-bp RAPD marker found to be strain-specific was sequenced, and two primer pairs specific to BM TO-A were constructed based on this sequence. In addition, we explored a more specific DNA region using inverse PCR, and designed a strain-specific primer set for use in real-time quantitative PCR (qPCR). These primer pairs were tested against 25 Bacillus subtilis strains and were found to be strain-specific. After examination of the detection limit and linearity of detection of BM TO-A in feces, the qPCR method and strain-specific primers were used to quantify BM TO-A in the feces of healthy volunteers who had ingested 3 10 8 colony forming unit (CFU) of BM TO-A per day in tablets. During the administration period, BM TO-A was detected in the feces of all 24 subjects, and the average number of BM TO-A detected using the culture method and qPCR was about 10 4.8 and 10 5.8 cells per gram of feces, respectively. Using the qPCR method, BM TO-A was detected in the feces of half of the subjects 3 d after withdrawal, and was detected in the feces of only one subject 1 week after withdrawal. These results suggest that the qPCR method using BM TO-A strain-specific primers is useful for the quantitative detection of this strain in feces.Key words Bacillus subtilis; strain-specific primer; quantitative polymerase chain reaction (PCR); human feces Probiotics are live microbial food supplements that beneficially affect the host by improving its microbial balance.

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Analysis of major band of Enterobacter sakazakii by ERIC-PCR and development of a species-specific PCR for detection of Ent. sakazakii in dry food samples

Cited by 15 publications

References 29 publications

Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism Typing

Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism Typing

The Cronobacter sp. in milk and dairy products: Detection and typing

Development of Strain-Specific PCR Primers for Quantitative Detection of <i>Bacillus mesentericus</i> Strain TO-A in Human Feces

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