Analysis of indole compounds from the fruiting bodies and the culture mycelia of Sarcodon imbricatus

Sułkowska-Ziaja, Katarzyna; Muszyńska, Bożena; Ekiert, Halina

doi:10.1016/j.myc.2013.08.001

Cited by 12 publications

(5 citation statements)

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“…l -tryptophan and 5-hydroxy- l -tryptophan identified in mycelial cultures are observed in in vitro cultures of numerous edible species of Sarcodon imbricatus, I. badia and Cantharellus cibarius [ 30 – 32 ]. The concentrations of these two indole derivatives are higher in the mycelial cultures of the above-mentioned species than in the cultures of F. betulina , with the exception of I. badia cultures.…”

Section: Resultsmentioning

confidence: 99%

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

et al. 2018

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Fomitopsis betulina (Bull.) B.K. Cui, M.L. Han & Y.C. Dai has been used for medicinal purposes for over 5000 years. Numerous studies have confirmed the biological activity of compounds found in this species. The purpose of this study was a comparative analysis of selected groups of metabolites in the extracts from fruiting bodies and mycelial cultures. Phenolic acids (syringic, gallic, p-hydroxybenzoic, 3,4-dihydrophenylacetic), indole compounds (l-tryptophan, 5-hydroxy-l-tryptophan, 5-methyltryptamine), sterols (ergosterol, ergosterol peroxide, hexestrol, cholecalciferol), and triterpenes (betulinic acid, betulin) were determined quantitatively by high performance liquid chromatography with UV–Vis/DAD detection, while fatty acids were assessed with the gas chromatography method. Cytotoxic activity against selected human cancer cell lines was determined using the lactate dehydrogenase test. Anti-inflammatory activity was evaluated on lipopolysaccharide activated A549 cells. Those extracts with anti-inflammatory activity were evaluated for their inhibition of pro-inflammatory enzymes. The mycelium extract exhibited significant cytotoxic activity against prostate cancer cells, while the fruiting body extract indicated a moderate effect on the viability of melanoma and prostate cancer. Incubation of lung epithelial cells with biomass extract significantly decreased cyclooxygenase-2 levels compared to LPS activated A549 cells. This paper is the first report of a comparative quantitative analysis of the metabolites in mycelial cultures and fruiting bodies. In addition, a novel element of this study is its comparison of the cytotoxic and anti-inflammatory activity of the obtained extracts. The results of comparing the composition and activity of mycelium and fruiting bodies shows that the cultures could be proposed as a potential biotechnological source for selected biologically active compounds.

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Section: Resultsmentioning

confidence: 99%

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

et al. 2018

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“…Antioxidant active ingredients in natural medicines mainly include polysaccharides, polyphenols, tetraterpenes, sterols, and vitamins, which realize antioxidant activity by scavenging free radicals, terminating the progress of chain oxidation, and improving the antioxidant capacity of the body [ 17 ]. Sarcodon imbricatus (SI), belonging to the family of Basidiomycotina and Aphyllophorales , is an edible and medicinal fungus, widely distributed in Central Europe and in North America [ 18 ] and also produced in Tibet, Gansu, Anhui, northwestern Yunnan, and western Sichuan of China [ 19 ]. Although SI is described to show various pharmacological activities including anti-inflammation and anticancer in the folk, previous studies mainly focused on its chemical component analysis and polysaccharide isolation [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise‐Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2‐Mediated Oxidative Stress

Wang

Zhang

et al. 2018

Oxidative Medicine and Cellular Longevity

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Sarcodon imbricatus (SI), a precious edible fungus, contains 35.22% of total sugar, 18.33% of total protein, 24 types of fatty acid, 16 types of amino acid, and 8 types of minerals. Encouragingly, it is rich in potential antioxidants such as total polyphenols (0.41%), total sterols (3.16%), and vitamins (0.44%). In the present study, the antifatigue properties of SI and its potential mechanisms of action were explored by the experiments on acute excise-treated mice and chronic fatigue syndrome (CFS) mice. SI (0.25, 0.5, and 1 g/kg) significantly enhanced exercise tolerance in the weight-loaded forced swimming test (FST) and rota-rod test (RRT) and reduced the immobility in the tail suspension test on CFS mice. SI markedly increased the levels of glycogen in the liver and adenosine triphosphate (ATP) in the liver and muscle and decreased the lactic acid (LD) and blood urea nitrogen (BUN) content in both acute swimming-treated mice and CFS mice. SI improved the endogenous cellular antioxidant enzyme contents in the two mouse models by improving the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels in serum, liver, and muscle, respectively. In CFS mice, the enhanced expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2), SOD1, SOD2, heme oxygenase-1 (HO-1), and catalase (CAT) in the liver were observed after a 32-day SI administration. Our data indicated that SI possessed antifatigue activity, which may be related to its ability to normalize energy metabolism and Nrf2-mediated oxidative stress. Consequently, SI can be expected to serve as a novel natural antifatigue supplement in health foods.

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“…Sarcodon imbricatus , a well-known edible fungus, has been widely used in Asian medicine because of its pharmacological activities ( Khaliq et al, 2016 , Vizzini et al, 2012 ). However, most studies regarding S. imbricatus have focused on the separation and analysis of its components ( Sułkowska-Ziaja et al, 2014 , Ishaq and Jafri, 2017 ); its immunomodulatory function has not previously been systematically studied.…”

Section: Introductionmentioning

confidence: 99%

Investigation on the immunomodulatory activities of Sarcodon imbricatus extracts in a cyclophosphamide (CTX)-induced immunosuppressanted mouse model

Meng

Wang

et al. 2017

Saudi Pharmaceutical Journal

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, an edible fungus, is widely used in Asian medicine because of its significant pharmacological activities. In the present study, we investigated the immunomodulatory effects of polysaccharide-enriched extracts (SP) in cyclophosphamide (CTX)-induced immunosuppressed mice. polysaccharide (AP) was used as a positive control. Compared with CTX-induced immunosuppressed mice, thirty-day SP treatment strongly enhanced the organ indexes of spleen and thymus and suppressed hind paw swelling. Both AP and SP increased the serum levels of immunoglobulin (IgA, IgG, and IgM), and suppressed the overproduction of interleukin-2 (IL-2). Moreover, SP reduced methane dicarboxylic aldehyde levels, and increased the total antioxidant capacity, superoxide dismutase, and glutathione peroxidase in both serum and liver tissues of CTX-induced immunosuppressed mice. extracts significantly improved immune function in CTX-induced immunosuppressed mice via modulation of oxidative systems.

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Analysis of indole compounds from the fruiting bodies and the culture mycelia of Sarcodon imbricatus

Cited by 12 publications

References 10 publications

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina

Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise‐Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2‐Mediated Oxidative Stress

Investigation on the immunomodulatory activities of Sarcodon imbricatus extracts in a cyclophosphamide (CTX)-induced immunosuppressanted mouse model

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