Analysis of genetic diversity in legumes germplasm using retrotransposon based molecular markers

Хапилина, О.Н.; Daniyarov, Asset; Amenov, Asset; Novakovskaya, A.P.; Turzhanova, Ainur; Tagimanova, Damelya; Filippova, Nadezhda; Kalendar, Ruslan

doi:10.11134/btp.2.2017.4

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…Similarly, Guilengue et al [43] analysed L. mutabilis using ISSR. Khapilina et al [44] succeeded in using REMAP and other retrotransposon analysis methods to estimate the genetic diversity of L. angustifolius.…”

Section: Discussion Of the Genetic Diversity Within Populations Of Lupinmentioning

confidence: 99%

Genetic Diversity in Invasive Populations of Lupinus polyphyllus Lindl. and Heracleum sosnowskyi Manden.

Osipova

Stepanova

Терешонок

et al. 2021

Biology

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In our study, two aggressive-invasive species, Lupinus polyphyllus Lindl. and Heracleum sosnowskyi Manden. from Russia and Ukraine, were investigated. The success in naturalization of both species is associated with human activities, since they have been used in agriculture and floriculture and both have qualities such as environmental tolerance, high fertility and phenotypic plasticity. The purpose of this study was to determine the level of genetic diversity of both species. For Heracleum sosnowskyi Manden., genetic diversity was compared in invasive and native populations. For Lupinus polyphyllus Lindl., the genetic diversity was compared in variety, feral and invasive populations. A genetic diversity was formulated using RAPD, ISSR and REMAP. For Heracleum sosnowskyi Manden., the average genetic diversity within the invasive population was similar (0.432), but slightly less (0.502) than within the native Caucasian population. This may suggest the successful naturalization of invaders and almost complete reconstruction of their genetic diversity. For Lupinus polyphyllus Lindl., the genetic diversity for the invasive population was the highest, with an average of 0.294, while for variety, it was the lowest, with an average of 0.194. The feral population had an intermediate place with an average of 0.248, which could suggest an increase of diversity in the process of naturalization.

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Section: Discussion Of the Genetic Diversity Within Populations Of Lupinmentioning

confidence: 99%

Genetic Diversity in Invasive Populations of Lupinus polyphyllus Lindl. and Heracleum sosnowskyi Manden.

Osipova

Stepanova

Терешонок

et al. 2021

Biology

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iPBS polymorphism of rare relict and endangered Allium species growing on the territory of Kazakhstan Altai

Rayzer¹,

Hapilina²

2020

Agrarian Bulletin of The

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Abstract. The purpose of the study. The estimation of genetic polymorphism of Kazakhstan populations of rare relict and endemic Allium species. The novelty of the research is the use of the modern molecular genetic iPBS (Inter- Primer Binding Site Polymorphism) method of DNA amplification to assess the genetic diversity of different populations of Allium sp., collected in their natural habitats in the Kazakhstan Altai. Methods. Samples of medicinal relict and endangered species A. ledebourianum, A. altaicum, A. microdiction were collected in the places of their natural growth in the territory of the Kazakhstan Altai. DNA was isolated from 3–5 day sterile seedlings using lysis STAB buffer with RNaseA. PBS primers were used to assess the genetic diversity of different populations of Allium spp. The amplification results obtained using different PBS primers were evaluated in the GenAlex 6.5 macro program for Excel. Results. The polymorphism of 16 genotypes of the rare relict and endangered Allium sp. was analyzed using 7 PBS primers. Clearly distinguishable amplicons were obtained, the number of which varied depending on the primer used. The dendrogram, based on UPGMA analysis, grouped the studied genotypes into 2 main clusters, one of which included samples from the A. altaicum population, and the second cluster included samples from the A. ledebourianum population. A. microdiction represented by one sample did not enter any cluster, and formed a basal branch in the dendrogram. The results of the study have revealed a high degree of iPBS polymorphism and genetic diversity in rare relict and endangered Allium sp. Practical significance. The use of the molecular genetic iPBS method allows to identify a high level of polymorphism, which can serve as a basis for the identification of various genotypes of the Allium sp., which will significantly supplement traditional preservation methods of natural populations of this genus.

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Analysis of genetic diversity in legumes germplasm using retrotransposon based molecular markers

Cited by 2 publications

References 13 publications

Genetic Diversity in Invasive Populations of Lupinus polyphyllus Lindl. and Heracleum sosnowskyi Manden.

Genetic Diversity in Invasive Populations of Lupinus polyphyllus Lindl. and Heracleum sosnowskyi Manden.

iPBS polymorphism of rare relict and endangered Allium species growing on the territory of Kazakhstan Altai

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