Analysis of flavonoid glycosides with potential medicinal properties on Bauhinia uruguayensis and Bauhinia forficata subspecies pruinosa.

Abstract

Several Bauhinia species are widely used in Southern South America in the treatment of infections, pain and several diseases including diabetes. Flavonoid compounds based on quercetin and kaempferol glycoside derivatives are believed to be responsible for their therapeutic properties. To investigate this, we have studied two native species from Argentina: B. uruguayensis (BU) and B. forficata subsp. pruinosa (BF). We have analyzed the major polyphenol components in hydro-methanolic extracts of leaves, by high performance liquid chromatography tandem mass spectrometry. Chromatographic analysis yielded five main compounds in BF, corresponding to rutinosides and rhamnosides derivatives of kaempferol and quercetin, which are considered chemotaxonomic markers and responsible for antioxidant activity. The presence of kaempferitrin, an antidiabetic agent, has been confirmed. In extracts of BU, four major compounds were identified as rhamnosides and galloyl derivates from quercetin and kaempferol. One of these compounds, quercitrin-3-rhamnoside may confer anti-inflammatory and analgesic properties to BU extracts.

Seasonal Flavonoid Profile and Kaempferitrin Content in the Leaf Extracts of <i>Bauhinia forficata</i> Subspecies <i>forficata</i> from Two Locations in Southeastern Brazil

“…Excepting peak 7 (20%), all others showed [M-H]-ion as the base peak. 4 Based on the UV typical profiles [31] and comparing MS data with those reported in [18] and [27]; K = kaempferol; Q = quercetin, I = isorhamnetin. Most spectra showed fragments corresponding to K (m/z 283) or Q (m/z 301) aglycones.…”

“…All these nuances have to be duly considered when aiming standardization of medicinal extracts [37]. [27]. However, with rare exceptions [22], such data usually do not accompany the set of information pro- of glycosylated aglycones present in each one.…”

“…Efforts have been made to develop and apply analytical methods to establish the qualitative and/or quantitative polyphenolic compounds [17] [18] [25]. In such a context, dosing of kaempferitrin content remains relevant, regarding the background of this compound as hypoglycemic agent [14] [15] [26] [27]. Overall, the results have shown a wide variation on kaempferitrin content and polyphenolic profiles among commercial samples and those from different locations and seasons, including the two B. forficata subspecies: forficata and pruinosa [18] [25] [26] [27] [28] [29].…”

Novel Therapeutic Uses of Legume Crops in Southern South America

“…Likewise, it is emphasized that herbal medicines have a variable concentration of active ingredients, which depends on population diversity, the phenological stage at the time of harvest, and the edaphoclimatic conditions in which they grow [35][36][37]. In our recent studies, one population analyzed from plant materials collected in the Botanical Garden, INTA, presented five glycoside derivatives of kaempferol and quercetin [38], whereas another population/specimens belonging to the same garden did not show the same bioactivity (unpublished data).…”

Isolation, characterization, and cytotoxic studies of secondary metabolites from the leaves of Bauhinia foveolata Dalzell: An endemic tree from the Western Ghats, India

“…Rutinoside and rhamnoside derivatives of kaempferol and quercetin, kaempferitin, were isolated from the leaves of B. uruguayensis and B. forficata subsp. Pruinosa (Santos et al, 2018).…”