Analysis of chicken primordial germ cells

Motono, Makoto; Ohashi, Takuya; Nishijima, Ken‐ichi; Iijima, Shinji

doi:10.1007/s10616-008-9156-x

Cited by 42 publications

(34 citation statements)

References 28 publications

(25 reference statements)

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“…In our hands, the number of PGCs remained approximately constant between HH5 and HH19. In agreement, Fujimoto and colleagues described 312 PGCs at HH10 (Fujimoto et al, 1976), Motono and colleagues referred to about 300 PGCs at HH13–HH16 (Motono et al, 2008) and Nakamura described embryos containing on average 300 PGCs from HH14 to HH20 (Nakamura et al, 1991). Together, we concluded that from HH5, when the PGCs are present in the germinal crescent, until HH19, when the PGCs have reached the genital ridges, the number of PGCs remained constant and range from 200–400.…”

Section: Discussionmentioning

confidence: 66%

“…SSEA1 has been used as an appropriate marker to identify and isolate PGCs from chicken embryos (Lee et al, 2011; Minematsu et al, 2008; Motono et al, 2008; Motono et al, 2010; Mozdziak et al, 2005; Petitte et al, 1997). However, by performing double immunofluorescence analyses, using antibodies against CVH and SSEA1, we have now demonstrated that although SSEA1 marked PGCs, it is only expressed by a fraction, albeit increasing, of CVH-positive PGCs between HH8–HH19, but not in the entire population of PGCs.…”

Section: Discussionmentioning

confidence: 99%

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Chicken primordial germ cells use the anterior vitelline veins to enter the embryonic circulation

et al. 2012

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SummaryDuring gastrulation, chicken primordial germ cells (PGCs) are present in an extraembryonic region of the embryo from where they migrate towards the genital ridges. This is also observed in mammals, but in chicken the vehicle used by the migratory PGCs is the vascular system. We have analysed the migratory pathway of chicken PGCs, focusing on the period of transition from the extraembryonic region to the intraembryonic vascular system.Our findings show that at Hamburger and Hamilton developmental stage HH12–HH14 the majority of PGCs concentrate axially in the sinus terminalis and favour transport axially via the anterior vitelline veins into the embryonic circulation. Moreover, directly blocking the blood flow through the anterior vitelline veins resulted in an accumulation of PGCs in the anterior region and a decreased number of PGCs in the genital ridges. We further confirmed the key role for the anterior vitelline veins in the correct migration of PGCs using an ex ovo culture method that resulted in defective morphogenetic development of the anterior vitelline veins.We propose a novel model for the migratory pathway of chicken PGCs whereby the anterior vitelline veins play a central role at the extraembryonic and embryonic interface. The chicken model of PGC migration through the vasculature may be a powerful tool to study the process of homing (inflammation and metastasis) due to the striking similarities in regulatory signaling pathways (SDF1–CXCR4) and the transient role of the vasculature.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Chicken primordial germ cells use the anterior vitelline veins to enter the embryonic circulation

et al. 2012

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“…It has been known that PGCs express genes associated with both pluripotency and germline properties. 19 To analyze characteristic gene expression profile of cPGC lines established in this study, we checked the expression of the chicken homologues of Oct4, Nanog, Vasa and Dazl. Most cPGC clones expressed germ cell specific and pluripotency marker genes as expected ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Establishment of An Efficient and Stable Transgene Expression System in Chicken Primordial Germ Cells

Yang¹,

Kim²

2012

Bulletin of the Korean Chemical Society

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Chicken primordial germ cells (cPGCs) are founder germ cells in embryonic stage of development that eventually give rise to sperms or oocytes. Currently cPGCs are only known cells enabling germline transmission in chicken and their cultivation protocols were recently established. Although genome modifications of chickens are now theoretically possible using cPGCs, there are still several hurdles to overcome to practically use cPGCs as mediators for chicken transgenesis. First, efficiency of gene delivery into cPGCs remains low with current methods. Second, there aregene silencing mechanisms against the expression of foreign genes in cPGCs. In this study, we successfully increased the efficiency of gene delivery in cPGCs by taking advantage of the TTAAspecific piggybac transposon system. Moreover, a pipette-type electroporator significantly enhanced transfection efficiency up to 5-fold compared withcuvette-type methods. Taken together, the technological advances in our study will provide practical benefits for the application to fulfill genetic modifications of chicken genome.

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“…In mouse, Blimp1 is essential for the specification of PGCs. Blimp1 was shown to be expressed in SSEA1 ＋ cells isolated from chicken embryos (Motono et al, 2008), suggesting that this gene could also play a role in chicken germ cell development.…”

Section: Germ Cell-specific Genes and Determinants Identified In Chickenmentioning

confidence: 99%

Primordial Germ Cell Technologies for Avian Germplasm Cryopreservation and Investigating Germ Cell Development

Glover

McGrew

2012

J. Poult. Sci.

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The germ cell lineage is unique as it is the cell lineage that ensures the continuation and the variation of genetic information from one generation to the next. Primordial germ cells are the stem cell precursors of this lineage differentiating into mature spermatozoa and oocytes in the adult. The recent development of cell culture conditions for the extended propagation of chicken primordial germ cells isolated from embryonic blood heralds the emergence of a technology that will have a considerable impact on the management of avian resources, efforts in avian species preservation and transgenic technologies. This review will address what is known of the formation of avian germ cells, the current state of the field, and discuss the prospects that this technology offers for both developmental biology and the preservation of avian germplasm.

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Analysis of chicken primordial germ cells

Cited by 42 publications

References 28 publications

Chicken primordial germ cells use the anterior vitelline veins to enter the embryonic circulation

Chicken primordial germ cells use the anterior vitelline veins to enter the embryonic circulation

Establishment of An Efficient and Stable Transgene Expression System in Chicken Primordial Germ Cells

Primordial Germ Cell Technologies for Avian Germplasm Cryopreservation and Investigating Germ Cell Development

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