Analysis of Bioadhesivity of Osteoblast Cells on Titanium Alloy Surface Modified by Nd:YAG Laser

Abstract

The surface microtopography and physical-chemical results of Ti6Al4V alloy were investigated in relation to bone cell response. Nd:YAG-laser-treated surfaces with (1.06 lm wavelength, 200 ls pulse duration, and a fluence of 140 Jcm À2 exhibited an improved hydrophilic behavior due to a lower contact angle compared with the control sample. Cell spreading on the implanted specimens was analyzed by scanning electron microscope (SEM), and their condition in a specific area was studied for 10 cells from three separate regions on the same specimen using an Image J Program software. The in vitro the in vivo tests provided some useful clinical and pathological information such as the number of adhered cells on the implant. The light microscopy assessment consisted of a complete morphological description of tissue response to the implants with different surface topography.

Surface morphology characterization of laser-induced titanium implants: lesson to enhance osseointegration process

“…From micro-mechanical point of view, the previous finding of enhanced properties [16,20,22] after laser treatment at 140 Jcm -2 compared to untreated, as well as 70 and 250 Jcm -2 samples, were consistent with new finding of significant difference between samples' surface morphologies. The higher surface hardness (850, 377 HVN for 140 Jcm -2 and untreated samples, respectively), enhanced corrosion resistance, lower contact angle (35°for 140 Jcm -2 and 70°for untreated sample) and higher surface tension (60 mNm -1 ) for 140 Jcm -2 compared to untreated surface (39 mNm -1 ) have been reported before.…”

“…From osseointegration point of view, the observation of change in density of cell network from mono-layer to multi-layer for 140 Jcm -2 samples [20,22] were consistent with the optimum height profile obtained in the sample (Figs. 2, 3).…”

“…Understanding the impact of morphology of the implant on micromechanical properties and implant osseointegration is an important step towards developing more-accurate multi-scale computational models for implant function, which can lead to improved strategies for tissue engineering and repair. Based on our previous studies significant improvements in micromechanical and biomedical properties of Ti64 were reported, however, appropriate surface morphology characterization was not reported [20,22]. Therefore the aim of this study was to characterize the morphology of Ti64 surface in order to achieve more information on surface texture alteration, which may affect osseointegration process after laser treatment.…”

“…Among them, surface roughness seems to play an important role, while its optimization enhances hydrophilicity of the implant surface [20,21]. Improvement of hydrophilicity followed by achieving smoother surface increases the accumulation of water molecules that results in higher blood protein's interaction with implant surface [22,23]. It was explained that increasing surface roughness supports greater amount of fibrin network expansion on the surface or enhances its strength of attachment, both promoting a better cell migration towards implant [24,25].…”

Characterization of a novel nanobiomaterial fabricated from HA, TiO2 and Al2O3 powders: an in vitro study

“…Therefore, it is used as filler in small bone defects and as a coating on metal implants such as titanium (Bai et al 2010a; Sadat-Shojai et al 2010; Sato et al 2006). Recent studies have reported improved ossification process and implant fixation through various methods including plasma spray and laser (Roy et al 2011; Topić et al 2006; Khosroshahi et al 2009, 2007). …”