Analysis of bacterial fermentation products by isotachophoresis

Hoeven, J.S. van der; Franken, Herman; Camp, Pamela J.; Dellebarre, C. W.

doi:10.1128/aem.35.1.17-23.1978

Cited by 31 publications

(8 citation statements)

References 6 publications

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“…Dry weight determinations were done at least in duplicate in separate cultures. Formic, acetic, succinic, lactic, citric and glutamic acids were determined with isotachophoresis [12]. Spectra of dithionite-reduced cytochromes in whole bacterial cells were obtained with an Aminco Chance spectrophotometer (American Instruments Co., Washington, U.S.A.) [13].…”

Section: Analytical Proceduresmentioning

confidence: 99%

Effects of oxygen on the growth and metabolism ofActinomyces viscosus

Jong

Hoeven

Kieboom

et al. 1988

FEMS Microbiology Letters

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Actinomyces viscosus is a predominant microorganism in dental plaque. It is, just as the oral Streptococcus spp., a saccharolytic and aero‐tolerant organism. We have investigated the effects of oxygen on the growth and metabolism of A. viscosus. To this end A. viscosus Ut 2 was grown in a glucose limited chemostat culture on a chemically defined medium (D= 0.2 h−1) with exposure to variable amounts of oxygen. The Yglucose increased from 62.5 g · mol−1 under anaerobic conditions to 149 g · mol−1 under aerobic conditions, while, concomitantly, the carbon recovery from acidic fermentation products decreased from 75% to 7%. Addition of [14C]glucose to the chemostat showed that the glucose, which was not converted to acidic fermentation products, was instead converted to carbon dioxide or used for the production of biomass. Under aerobic and anaerobic conditions identical cytochrome spectra, containing only two cytochrome b‐type absorption bands, were found. It was concluded that electron transport phosphorylation probably occurs both under aerobic and anaerobic conditions. Anaerobically, fumarate served as the electron acceptor, while the high growth yields observed under aerobic conditions are likely to be explained by citric acid cycle activity coupled to electron transport phosphorylation.

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Section: Analytical Proceduresmentioning

confidence: 99%

Effects of oxygen on the growth and metabolism ofActinomyces viscosus

Jong

Hoeven

Kieboom

et al. 1988

FEMS Microbiology Letters

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“…Samples were taken each minute, through a 0.22 ttm Millipore filter, from immediately before to 20 min after the glucose pulse. Acidic fermentation products were analysed using isotachophoresis [10]. The leading electrolyte was 4-aminobutyric acid (5 mM) at pH 4.1, and the terminating electrolyte was octanoic acid (4 mM) at pH 5.0.…”

Section: Chemical Analysesmentioning

confidence: 99%

Competition between oral species in the chemostat under alternating conditions of glucose limitation and excess

Vanderhoeven¹,

DeJong²,

Camp³

et al. 1985

FEMS Microbiology Letters

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Oral Streptococcus species experience carbohydrate limitation interrupted by periods of substrate excess following food intake by the host. To investigate the competitiveness of various streptococcal species under fluctuating carbohydrate supply, 2-membered chemostat cultures were run.Under continuous limitation of glucose or sucrose, all 6 Streptococcus mutans test strains were outcompeted by Streptococcus sanguis P4A7 or Streptococcus milleri B448. This indicated that S. mutans had a lower affinity for glucose and sucrose than S. sanguis and S. milleri.Mixed cultures were then subjected to hourly pulses with glucose. Under these conditions S. mutans Ny344 competed successfully with S. milleri B448, but still lost the competition against S. sanguis P4A7. The streptococci responded to pulses by taking up glucose at the maximum rate almost instantaneously. S. sanguis P4A7 had the highest rate of glucose uptake while the qmax value of S. mutans Ny344 was higher than that Of S. milleri B448. This suggested a causal relationship between qmax and competitiveness.

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“…Ethanol was measured enzymatically (Boehringer Mannheim Biochemicals, Indianapolis, Ind.). Formate, acetate, and lactate were determined isotachophoretically (38). Volatile products were also analyzed by gas-liquid chromatography (18).…”

Section: Methodsmentioning

confidence: 99%

Estimation of growth parameters for some oral bacteria grown in continuous culture under glucose-limiting conditions

Rogers¹,

Jong²,

Zilm³

et al. 1986

Infect Immun

Self Cite

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The coexistence of bacteria in natural environments can often be explained in terms of competition for a growth-limiting substrate(s), and the outcome of such competition depends upon relevant growth parameters such as substrate affinity and yield. Dental plaque bacteria are frequently carbon and energy limited. Growth parameters for seven oral Sreptococcus species and one Actinomyces viscosus strain were estimated under glucose-limited conditions in continuous culture. In all strains, mixed-acid fermentation occurred at low growth rates, while amounts of lactate increased at higher growth rates. Two important growth parameters, ulmax and Ygi,cose, were very similar in the two serotype c Streptococcus mutans strains (T8 and Ingbritt), one of the serotype d/g Streptococcus mutans strains (OMZ65), and the two Streptococcus milledi strains (699B3 and B448). Two other serotype d/g S. mutans strains (KIR and B13) were divergent from this group and had lower ILmax values and a lower YglU,O,e. The maintenance energy coefficients were lower in the S. mutans serotype c

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Analysis of bacterial fermentation products by isotachophoresis

Cited by 31 publications

References 6 publications

Effects of oxygen on the growth and metabolism ofActinomyces viscosus

Effects of oxygen on the growth and metabolism ofActinomyces viscosus

Competition between oral species in the chemostat under alternating conditions of glucose limitation and excess

Estimation of growth parameters for some oral bacteria grown in continuous culture under glucose-limiting conditions

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