volume 39, issue 2-3, P380-388 2005
DOI: 10.1016/j.watres.2004.09.025
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Emilie Lyautey, Bénédicte Lacoste, Loïc Ten-Hage, Jean-Luc Rols, Frédéric Garabetian

Abstract: Reliability of bacterial diversity assessment using polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) analysis of 16S rDNA fragments was evaluated for a particular complex microbial assemblage: river epilithic biofilm. By comparing 3 routine protocols on replicates of one river biofilm sample, we found that common DNA extraction procedures gave comparable diversity (from 28.0 to 30.7 bands detected) and community composition (> 75% of homology) despite differences in the total amou…

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