Analysis of abscisic acid in wheat leaves by a combination of high-performance liquid chromatography and radioimmunoassay

Immunoaffinity columns for isolation of abscisic acid in conifer seedlings

“…In the present study, we used cis,trans (±) ABA (Weiler 1979) to prepare the antigen, since previous 4.00 3.00- experience had shown (Walton et al 1979, Kannangara et al 1984) that polyclonal antibodies to naturally occurring cis,trans (-I-) ABA can be produced by using cis,trans (±) ABA as a hapten. Hydridomas were screened by competition ELISA where the cell culture fluids were preincubated with cis,trans (±) ABA prior to incubation in conjugate-coated microwell plates.…”

“…For example, Ross et al (1987) validated their enzymelinked immunoassay (ELISA) estimations of ABA in semi-purified extracts of pea cotyledons by comparing to ABA estimations of the same tissue made by gas chromatograph equipped with an electron capture detector. Kannangara et al (1984) compared radioimmunoassay estimations of ABA in wheat leaves to estima-tions of ABA in the same tissue made by high-performance hquid chromatograph (HPLC) equipped with an UV detector. Knox and Galfre (1986) used monoclonal antibodies to separate enantiomers of ABA.…”

Immunoaffinity columns for isolation of abscisic acid in conifer seedlings

“…In the present study, we used cis,trans (±) ABA (Weiler 1979) to prepare the antigen, since previous 4.00 3.00- experience had shown (Walton et al 1979, Kannangara et al 1984) that polyclonal antibodies to naturally occurring cis,trans (-I-) ABA can be produced by using cis,trans (±) ABA as a hapten. Hydridomas were screened by competition ELISA where the cell culture fluids were preincubated with cis,trans (±) ABA prior to incubation in conjugate-coated microwell plates.…”

“…For example, Ross et al (1987) validated their enzymelinked immunoassay (ELISA) estimations of ABA in semi-purified extracts of pea cotyledons by comparing to ABA estimations of the same tissue made by gas chromatograph equipped with an electron capture detector. Kannangara et al (1984) compared radioimmunoassay estimations of ABA in wheat leaves to estima-tions of ABA in the same tissue made by high-performance hquid chromatograph (HPLC) equipped with an UV detector. Knox and Galfre (1986) used monoclonal antibodies to separate enantiomers of ABA.…”

Experimental Studies of the Factors That Influence 1-Naphthaleneacetamide Determination by Micelle-stabilized Room-temperature Phosphorescence

“…[5][6][7][8] As a rule, the last procedures offer greater selectivity, the use of smaller amounts of sample and shorter analysis times.…”

“…The methods most frequently used for this type of analysis include biological assays, 3 immunological assays 4 and physical detection procedures. [5][6][7][8] As a rule, the last procedures offer greater selectivity, the use of smaller amounts of sample and shorter analysis times.…”

An indirect enzyme-linked immunosorbent assay for (+)-abscisic acid in Citrus, Ricinus, and Xanthium leaves

“…Immunoassays provide a technique to use the selectivity of Ab to identify and quantify the low physiological concentrations of hormones in plant material. Several RIA methods for ABA quantitation have been reported (Kannangara et al, 1984; Le Page-Degivry and Bulard, 1984; Leroux et al, 1985;Walton et al, 1979; Weiler, 1979Weiler, , 1980; Rosher et al, 1985). Direct ELISA assays for free and conjugated ABA using PAb have been reported (Daie and Wyse, 1982; Weiler, 1982;Harris and Dugger, 1986).…”

Immunoaffinity columns for isolation of abscisic acid in conifer seedlings

“…In the present study, we used cis,trans (±) ABA (Weiler 1979) to prepare the antigen, since previous 4.00 3.00- experience had shown (Walton et al 1979, Kannangara et al 1984) that polyclonal antibodies to naturally occurring cis,trans (-I-) ABA can be produced by using cis,trans (±) ABA as a hapten. Hydridomas were screened by competition ELISA where the cell culture fluids were preincubated with cis,trans (±) ABA prior to incubation in conjugate-coated microwell plates.…”

“…For example, Ross et al (1987) validated their enzymelinked immunoassay (ELISA) estimations of ABA in semi-purified extracts of pea cotyledons by comparing to ABA estimations of the same tissue made by gas chromatograph equipped with an electron capture detector. Kannangara et al (1984) compared radioimmunoassay estimations of ABA in wheat leaves to estima-tions of ABA in the same tissue made by high-performance hquid chromatograph (HPLC) equipped with an UV detector. Knox and Galfre (1986) used monoclonal antibodies to separate enantiomers of ABA.…”

Experimental Studies of the Factors That Influence 1-Naphthaleneacetamide Determination by Micelle-stabilized Room-temperature Phosphorescence

“…[5][6][7][8] As a rule, the last procedures offer greater selectivity, the use of smaller amounts of sample and shorter analysis times.…”

“…The methods most frequently used for this type of analysis include biological assays, 3 immunological assays 4 and physical detection procedures. [5][6][7][8] As a rule, the last procedures offer greater selectivity, the use of smaller amounts of sample and shorter analysis times.…”

An indirect enzyme-linked immunosorbent assay for (+)-abscisic acid in Citrus, Ricinus, and Xanthium leaves

“…Immunoassays provide a technique to use the selectivity of Ab to identify and quantify the low physiological concentrations of hormones in plant material. Several RIA methods for ABA quantitation have been reported (Kannangara et al, 1984; Le Page-Degivry and Bulard, 1984; Leroux et al, 1985;Walton et al, 1979; Weiler, 1979Weiler, , 1980; Rosher et al, 1985). Direct ELISA assays for free and conjugated ABA using PAb have been reported (Daie and Wyse, 1982; Weiler, 1982;Harris and Dugger, 1986).…”

Immunoaffinity columns for isolation of abscisic acid in conifer seedlings

“…In the present study, we used cis,trans (±) ABA (Weiler 1979) to prepare the antigen, since previous 4.00 3.00- experience had shown (Walton et al 1979, Kannangara et al 1984) that polyclonal antibodies to naturally occurring cis,trans (-I-) ABA can be produced by using cis,trans (±) ABA as a hapten. Hydridomas were screened by competition ELISA where the cell culture fluids were preincubated with cis,trans (±) ABA prior to incubation in conjugate-coated microwell plates.…”

“…For example, Ross et al (1987) validated their enzymelinked immunoassay (ELISA) estimations of ABA in semi-purified extracts of pea cotyledons by comparing to ABA estimations of the same tissue made by gas chromatograph equipped with an electron capture detector. Kannangara et al (1984) compared radioimmunoassay estimations of ABA in wheat leaves to estima-tions of ABA in the same tissue made by high-performance hquid chromatograph (HPLC) equipped with an UV detector. Knox and Galfre (1986) used monoclonal antibodies to separate enantiomers of ABA.…”

Experimental Studies of the Factors That Influence 1-Naphthaleneacetamide Determination by Micelle-stabilized Room-temperature Phosphorescence

“…[5][6][7][8] As a rule, the last procedures offer greater selectivity, the use of smaller amounts of sample and shorter analysis times.…”

“…The methods most frequently used for this type of analysis include biological assays, 3 immunological assays 4 and physical detection procedures. [5][6][7][8] As a rule, the last procedures offer greater selectivity, the use of smaller amounts of sample and shorter analysis times.…”

An indirect enzyme-linked immunosorbent assay for (+)-abscisic acid in Citrus, Ricinus, and Xanthium leaves

“…Immunoassays provide a technique to use the selectivity of Ab to identify and quantify the low physiological concentrations of hormones in plant material. Several RIA methods for ABA quantitation have been reported (Kannangara et al, 1984; Le Page-Degivry and Bulard, 1984; Leroux et al, 1985;Walton et al, 1979; Weiler, 1979Weiler, , 1980; Rosher et al, 1985). Direct ELISA assays for free and conjugated ABA using PAb have been reported (Daie and Wyse, 1982; Weiler, 1982;Harris and Dugger, 1986).…”