Analysis of a 26 kb region on the left arm of yeast chromosome XV

Phenotypic Switching in Candida glabrata Involves Phase-Specific Regulation of the Metallothionein Gene MT-II and the Newly Discovered Hemolysin Gene HLP

“…The fragment showed 76% identity at the deduced amino acid level to the S. cerevisiae gene YOL060c (25). It was also similar to hemolysin-like genes from a variety of organisms, ranging in complexity from bacteria to nematodes (Fig.…”

“…HLP, a gene for a hemolysin-like protein, also regulated by switching. Using gene-specific primers for the S. cerevisiae SLF1 gene (57), we performed a PCR with C. glabrata genomic DNA as a template and serendipitously cloned a partial DNA fragment of a C. glabrata homolog of the S. cerevisiae gene YOL060c (25), which encodes a putative protein with three transmembrane domains. The cloned DNA fragment was 1,586 nucleotides in length.…”

Phenotypic Switching in Candida glabrata Involves Phase-Specific Regulation of the Metallothionein Gene MT-II and the Newly Discovered Hemolysin Gene HLP

“…The fragment showed 76% identity at the deduced amino acid level to the S. cerevisiae gene YOL060c (25). It was also similar to hemolysin-like genes from a variety of organisms, ranging in complexity from bacteria to nematodes (Fig.…”

“…HLP, a gene for a hemolysin-like protein, also regulated by switching. Using gene-specific primers for the S. cerevisiae SLF1 gene (57), we performed a PCR with C. glabrata genomic DNA as a template and serendipitously cloned a partial DNA fragment of a C. glabrata homolog of the S. cerevisiae gene YOL060c (25), which encodes a putative protein with three transmembrane domains. The cloned DNA fragment was 1,586 nucleotides in length.…”

PRS1 is a key member of the gene family encoding phosphoribosylpyrophosphate synthetase in Saccharomyces cerevisiae

“…In the course of the EU Yeast Genome Sequencing Project Feldmann et al (1994) detected a PRS-like ORF on chromosome XV (Mannhaupt et al 1996). Interestingly, Prs5p possesses not one but two NHR regions; one is located between the divalent cation binding site and the PRPP binding site, as is the case for NHR of Prs1p.…”

Zinc finger transcriptional activators of yeasts

“… The first category contains a type of Zfs close to the motif described for transactivators but with some atypical parts; such is the Stp group (Stp1p–Stp4p) of which one member, Stp1p, is involved in RNA splicing [68–70]. The second category contains Zf motifs dispersed along the protein, where amino acids which are not directly related to the critical Cys and His of the finger are quite divergent. Within this second category, one can find a variety of proteins whose function concerns proteasomes (Ylr387cp), DNA repair (Rad18p) [71], control of nuclear protein localization (Sfp1p) [72], control of NaCl resistance (Psh1p) [73], pre‐mRNA splicing (Prp9p) or chaperoning (Ynl227cp) [74–76]. Two other proteins (Ybr267wp, Set5p) belonging to this class have not yet been functionally characterized [77]. Finally the last category contains only one finger with very few conserved amino acids and a great diversity of length between the two Cys and His of the classical motif.…”

“…The second category contains Zf motifs dispersed along the protein, where amino acids which are not directly related to the critical Cys and His of the finger are quite divergent. Within this second category, one can find a variety of proteins whose function concerns proteasomes (Ylr387cp), DNA repair (Rad18p) [71], control of nuclear protein localization (Sfp1p) [72], control of NaCl resistance (Psh1p) [73], pre‐mRNA splicing (Prp9p) or chaperoning (Ynl227cp) [74–76]. Two other proteins (Ybr267wp, Set5p) belonging to this class have not yet been functionally characterized [77].…”

Phenotypic Switching in Candida glabrata Involves Phase-Specific Regulation of the Metallothionein Gene MT-II and the Newly Discovered Hemolysin Gene HLP

“…The fragment showed 76% identity at the deduced amino acid level to the S. cerevisiae gene YOL060c (25). It was also similar to hemolysin-like genes from a variety of organisms, ranging in complexity from bacteria to nematodes (Fig.…”

“…HLP, a gene for a hemolysin-like protein, also regulated by switching. Using gene-specific primers for the S. cerevisiae SLF1 gene (57), we performed a PCR with C. glabrata genomic DNA as a template and serendipitously cloned a partial DNA fragment of a C. glabrata homolog of the S. cerevisiae gene YOL060c (25), which encodes a putative protein with three transmembrane domains. The cloned DNA fragment was 1,586 nucleotides in length.…”

PRS1 is a key member of the gene family encoding phosphoribosylpyrophosphate synthetase in Saccharomyces cerevisiae

“…In the course of the EU Yeast Genome Sequencing Project Feldmann et al (1994) detected a PRS-like ORF on chromosome XV (Mannhaupt et al 1996). Interestingly, Prs5p possesses not one but two NHR regions; one is located between the divalent cation binding site and the PRPP binding site, as is the case for NHR of Prs1p.…”

Zinc finger transcriptional activators of yeasts

“… The first category contains a type of Zfs close to the motif described for transactivators but with some atypical parts; such is the Stp group (Stp1p–Stp4p) of which one member, Stp1p, is involved in RNA splicing [68–70]. The second category contains Zf motifs dispersed along the protein, where amino acids which are not directly related to the critical Cys and His of the finger are quite divergent. Within this second category, one can find a variety of proteins whose function concerns proteasomes (Ylr387cp), DNA repair (Rad18p) [71], control of nuclear protein localization (Sfp1p) [72], control of NaCl resistance (Psh1p) [73], pre‐mRNA splicing (Prp9p) or chaperoning (Ynl227cp) [74–76]. Two other proteins (Ybr267wp, Set5p) belonging to this class have not yet been functionally characterized [77]. Finally the last category contains only one finger with very few conserved amino acids and a great diversity of length between the two Cys and His of the classical motif.…”

“…The second category contains Zf motifs dispersed along the protein, where amino acids which are not directly related to the critical Cys and His of the finger are quite divergent. Within this second category, one can find a variety of proteins whose function concerns proteasomes (Ylr387cp), DNA repair (Rad18p) [71], control of nuclear protein localization (Sfp1p) [72], control of NaCl resistance (Psh1p) [73], pre‐mRNA splicing (Prp9p) or chaperoning (Ynl227cp) [74–76]. Two other proteins (Ybr267wp, Set5p) belonging to this class have not yet been functionally characterized [77].…”

Phenotypic Switching in Candida glabrata Involves Phase-Specific Regulation of the Metallothionein Gene MT-II and the Newly Discovered Hemolysin Gene HLP

“…The fragment showed 76% identity at the deduced amino acid level to the S. cerevisiae gene YOL060c (25). It was also similar to hemolysin-like genes from a variety of organisms, ranging in complexity from bacteria to nematodes (Fig.…”

“…HLP, a gene for a hemolysin-like protein, also regulated by switching. Using gene-specific primers for the S. cerevisiae SLF1 gene (57), we performed a PCR with C. glabrata genomic DNA as a template and serendipitously cloned a partial DNA fragment of a C. glabrata homolog of the S. cerevisiae gene YOL060c (25), which encodes a putative protein with three transmembrane domains. The cloned DNA fragment was 1,586 nucleotides in length.…”

PRS1 is a key member of the gene family encoding phosphoribosylpyrophosphate synthetase in Saccharomyces cerevisiae

“…In the course of the EU Yeast Genome Sequencing Project Feldmann et al (1994) detected a PRS-like ORF on chromosome XV (Mannhaupt et al 1996). Interestingly, Prs5p possesses not one but two NHR regions; one is located between the divalent cation binding site and the PRPP binding site, as is the case for NHR of Prs1p.…”

Zinc finger transcriptional activators of yeasts

“… The first category contains a type of Zfs close to the motif described for transactivators but with some atypical parts; such is the Stp group (Stp1p–Stp4p) of which one member, Stp1p, is involved in RNA splicing [68–70]. The second category contains Zf motifs dispersed along the protein, where amino acids which are not directly related to the critical Cys and His of the finger are quite divergent. Within this second category, one can find a variety of proteins whose function concerns proteasomes (Ylr387cp), DNA repair (Rad18p) [71], control of nuclear protein localization (Sfp1p) [72], control of NaCl resistance (Psh1p) [73], pre‐mRNA splicing (Prp9p) or chaperoning (Ynl227cp) [74–76]. Two other proteins (Ybr267wp, Set5p) belonging to this class have not yet been functionally characterized [77]. Finally the last category contains only one finger with very few conserved amino acids and a great diversity of length between the two Cys and His of the classical motif.…”

“…The second category contains Zf motifs dispersed along the protein, where amino acids which are not directly related to the critical Cys and His of the finger are quite divergent. Within this second category, one can find a variety of proteins whose function concerns proteasomes (Ylr387cp), DNA repair (Rad18p) [71], control of nuclear protein localization (Sfp1p) [72], control of NaCl resistance (Psh1p) [73], pre‐mRNA splicing (Prp9p) or chaperoning (Ynl227cp) [74–76]. Two other proteins (Ybr267wp, Set5p) belonging to this class have not yet been functionally characterized [77].…”