Analysis and Selection of Microsatellites Markers for Individual Traceability System in Hanwoo

임현태,; 민희식,; 문원곤,; 이재봉,; 김재환,; 조인철,; 이학교,; 이용욱,; 이정규,; 전진태,

doi:10.5187/jast.2005.47.4.491

Cited by 10 publications

(1 citation statement)

References 15 publications

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“…As such, methods based on other DNA markers such as microsatellite (MS) markers - recommended by the International Society of Animal Genetics (ISAG) for analysis of polymorphism in cattle - are widely used in Hanwoo traceability research (Lee et al ., 2004; Lee et al, 2008; Lim et al ., 2005; Yoon et al ., 2005). Currently, single nucleotide polymorphism (SNP) markers, which were found in large numbers through decoding of the entire nucleotide sequence of the bovine chromosome, are distributed in a readily usable DNA chip form (Ball et al ., 2010; Sasazaki et al ., 2011; Van Eenennaam et al ., 2007).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Microsatellite and Single Nucleotide Polymorphism Methods for Discriminating among Hanwoo (Korean Native Cattle), Imported, and Crossbred Beef in Korea

Heo¹,

Ko²,

Seo³

et al. 2014

Korean Journal for Food Science of Animal Resources

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The identity of 45 Hanwo and 47 imported beef (non-Hanwoo) samples from USA and Australia were verified using the microsatellite (MS) marker and single nucleotide polymorphism (SNP) methods. Samples were collected from 19 supermarkets located in the city of Seoul and Gyeonggi province, South Korea, from 2009 to 2011. As a result, we obtained a 100% concordance rate between the MS and SNP methods for identifying Hanwoo and non-Hanwoo beef. The MS method presented a 95% higher individual discriminating value for Hanwoo (97.8%) than for non-Hanwoo (61.7%) beef. For further comparison of the MS and SNP methods, blood samples were collected and tested from 54 Hanwoo × Holstein crossbred cattle (first, second, and third generations). By using the SNP and MS methods, we correctly identified all of the first-generation crossbred cattle as non-Hanwoo; in addition, among the second and third generation crossbreds, the ratio identified as Hanwoo was 20% and 10%, respectively. The MS method used in our study provides more information, but requires sophisticated techniques during each experimental process. By contrast, the SNP method is simple and has a lower error rate. Our results suggest that the MS and SNP methods are useful for discriminating Hanwoo from non-Hanwoo breeds.

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