2010
DOI: 10.1016/j.jim.2009.09.012
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Abstract: Telomeres are the DNA–protein complexes that protect the ends of eukaryotic chromosomes. The cellular enzyme telomerase counteracts telomere shortening by adding telomeric DNA. A growing body of literature links shorter telomere length and lower telomerase activity with various age-related diseases and earlier mortality. Thus, leukocyte telomere length (LTL) and telomerase activity are emerging both as biomarkers and contributing factors for age-related diseases. However, no clinical study has directly examine… Show more

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Cited by 367 publications
(354 citation statements)
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“…These data reinforce the hypothesis that cellular senescence associated with a proinflammatory status could play a role in vascular dysfunction involved in age-related diseases, such as CHF. Interestingly, the increased expression of miR-146a did not reach significant levels in leukocytes obtained from the same setting of patients, which may be explained by the heterogeneity of leukocyte subpopulations (Lin et al 2010). We also observed a 2-fold over-expression of plasma levels of miR-146a in CHF patients.…”
Section: Discussionmentioning
confidence: 61%
“…These data reinforce the hypothesis that cellular senescence associated with a proinflammatory status could play a role in vascular dysfunction involved in age-related diseases, such as CHF. Interestingly, the increased expression of miR-146a did not reach significant levels in leukocytes obtained from the same setting of patients, which may be explained by the heterogeneity of leukocyte subpopulations (Lin et al 2010). We also observed a 2-fold over-expression of plasma levels of miR-146a in CHF patients.…”
Section: Discussionmentioning
confidence: 61%
“…Counts were standardized to 1 Â 10 6 cells by haemocytometer and stored at 2808C in 1X CHAPS lysis buffer. Telomerase assays were conducted as reported in Lin et al [23], briefly: Gel-TRAP reactions were performed by telomerase repeat amplification protocol (TRAPeze telomerase detection kit, Upstate/CHEMICON) using 2000-10 000 cells and run on 10 per cent polyacrylamide-8M urea sequencing gels. Gels were exposed to a phosphorimager plate overnight and scanned.…”
Section: Methodsmentioning
confidence: 99%
“…DNA was prepared from whole blood using Maxwell 16 blood DNA purification kit (Promega Biotech, Stockholm, Sweden) and sent to the Blackburn laboratory at the University of California, San Francisco, where telomere length assays were conducted, as previously described (Lin et al 2010). …”
Section: Telomere Length Measurement Assaymentioning
confidence: 99%